FastQCFastQC Report
Thu 26 May 2016
SRR1547200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547200_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1190739
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52550.4413225736286458No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14070.11816191457573826No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA13180.11068756461323599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC207.856594E-444.00000412
TATTCGC207.856594E-444.00000412
CGCGTAT207.856594E-444.00000434
CGACGTC302.527795E-644.018
TAATGCG600.044.01
ATCGAAT302.527795E-644.015
CGGTCTA1450.040.96551531
CGTTTTT36950.040.7253071
TATAGCG1300.040.6153871
TACGGGA2250.040.088894
TCGTACA557.8216544E-1140.034
TACGAAT1950.039.4871812
GGCGATA4900.038.1632658
GCGATAT1350.037.4814839
ATAGCGG2350.037.446812
ATTAGCG1000.037.3999981
TAAGGGA12300.037.3821144
GGGCGAT25250.037.378227
CACGACG1650.037.33333226
ATAGGGA14150.036.8480574
Produced by FastQC (version 0.11.5)