##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547200_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1190739
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.57074304276588	31.0	30.0	31.0	28.0	33.0
2	30.89790037951222	31.0	31.0	33.0	28.0	34.0
3	31.078093520074507	31.0	31.0	34.0	28.0	34.0
4	34.91102752156434	35.0	35.0	37.0	32.0	37.0
5	34.70484631812681	35.0	35.0	37.0	32.0	37.0
6	34.7366307813887	35.0	35.0	37.0	32.0	37.0
7	35.35833041497759	35.0	35.0	37.0	33.0	37.0
8	34.31871803980553	35.0	35.0	37.0	32.0	37.0
9	36.41493811826101	39.0	37.0	39.0	32.0	39.0
10	35.80116633451999	37.0	35.0	39.0	30.0	39.0
11	35.6057255200342	37.0	35.0	39.0	30.0	39.0
12	35.199959856862	37.0	34.0	39.0	30.0	39.0
13	35.01324219665267	37.0	34.0	39.0	28.0	39.0
14	35.89494759136973	38.0	34.0	40.0	27.0	41.0
15	36.079302013287545	38.0	34.0	40.0	30.0	41.0
16	36.16337753277586	38.0	34.0	40.0	30.0	41.0
17	36.07993439368325	38.0	34.0	40.0	30.0	41.0
18	36.011320700842084	38.0	34.0	40.0	30.0	41.0
19	36.00241110772386	38.0	34.0	40.0	29.0	41.0
20	35.88476651894328	38.0	34.0	40.0	29.0	41.0
21	35.69767346160661	38.0	34.0	40.0	29.0	41.0
22	35.652057251841086	37.0	34.0	40.0	29.0	41.0
23	35.53145063695739	37.0	34.0	40.0	29.0	41.0
24	35.43711258302617	37.0	34.0	40.0	28.0	41.0
25	35.24056909196726	37.0	33.0	40.0	27.0	41.0
26	35.06302724610515	37.0	33.0	40.0	27.0	41.0
27	34.854246816472795	37.0	33.0	40.0	26.0	41.0
28	34.85065576923238	37.0	33.0	40.0	26.0	41.0
29	34.66437565243097	36.0	33.0	40.0	25.0	41.0
30	34.7139121167611	37.0	33.0	40.0	25.0	41.0
31	34.68297502643316	37.0	33.0	40.0	25.0	41.0
32	34.48958671883595	37.0	33.0	40.0	25.0	41.0
33	34.34540566824468	37.0	33.0	40.0	24.0	41.0
34	34.227475542499235	37.0	33.0	40.0	24.0	41.0
35	34.04822047484797	36.0	32.0	40.0	23.0	41.0
36	34.01061861583437	36.0	33.0	40.0	23.0	41.0
37	34.03856680599191	36.0	33.0	40.0	23.0	41.0
38	34.04863702289083	36.0	33.0	40.0	23.0	41.0
39	33.94413973171283	36.0	32.0	40.0	23.0	41.0
40	33.84110287812862	36.0	32.0	40.0	23.0	41.0
41	33.85111010893235	36.0	32.0	40.0	23.0	41.0
42	33.69231376481328	36.0	32.0	40.0	23.0	41.0
43	33.73174054095818	36.0	32.0	40.0	23.0	41.0
44	33.42852631852992	35.0	32.0	39.0	22.0	41.0
45	33.34295844849291	35.0	31.0	39.0	22.0	41.0
46	33.27975736076504	35.0	31.0	39.0	22.0	41.0
47	33.19555167001333	35.0	31.0	39.0	22.0	41.0
48	33.20685809400717	35.0	31.0	39.0	22.0	41.0
49	33.18950500487512	35.0	31.0	39.0	22.0	41.0
50	32.61199221659827	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	7.0
13	13.0
14	28.0
15	61.0
16	176.0
17	448.0
18	902.0
19	1871.0
20	3123.0
21	4841.0
22	7048.0
23	9923.0
24	13824.0
25	18532.0
26	23999.0
27	28747.0
28	33777.0
29	39345.0
30	46769.0
31	55820.0
32	66989.0
33	81472.0
34	108789.0
35	120513.0
36	110338.0
37	128350.0
38	147528.0
39	137506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.64453587226084	23.56796913513373	27.23804292964285	14.549452062962581
2	31.734326330119362	25.40674320736954	28.779858558424642	14.079071904086454
3	29.577850393747074	26.42048341408151	30.096687855189085	13.904978336982326
4	26.5431803275109	28.554872226407298	29.336319714059922	15.565627732021877
5	22.562291148606032	32.928710657835175	29.43810524388636	15.070892949672428
6	21.07640717235263	41.91741431161657	26.464321736333485	10.541856779697314
7	89.02664647752361	3.5035385588277532	6.0059341299814655	1.4638808336671596
8	86.75083288613206	5.86207388856836	5.717037906711715	1.6700553185878684
9	83.77360613870883	5.066769459974016	7.995706867751876	3.16391753356529
10	40.97740982700659	29.72330628290499	15.385655462700054	13.91362842738837
11	35.36845605964027	25.285306015843943	22.957171974714864	16.389065949800923
12	32.695746087093816	22.39600785730542	26.941084486188828	17.96716156941194
13	24.624707849495145	28.58426573749579	27.492758698589697	19.298267714419364
14	20.857215561092733	31.223970996162887	28.45325465950137	19.46555878324301
15	20.418664375652433	23.792115652548542	36.62456676064192	19.164653211157106
16	22.869159404369892	21.950822136505145	34.823500364059626	20.356518095065333
17	23.017386681716147	21.731378580864487	28.570156852173312	26.68107788524605
18	24.551559997614927	23.382957978196732	30.69555964825205	21.369922375936287
19	28.246996193120406	25.041591818190216	26.009142221763128	20.702269766926253
20	30.235341246066515	24.31557209430446	25.153455123246992	20.29563153638203
21	26.466421272839806	26.48011025086102	27.05496334629167	19.998505130007498
22	25.46057532339161	24.03692160918556	26.457519238053006	24.04498382936983
23	23.585605241786823	27.320848649452145	26.039711473295156	23.053834635465876
24	23.59400338781211	24.772599201000386	31.32987161754171	20.303525793645793
25	23.347685764890542	25.615185191717078	29.180534105290917	21.856594938101466
26	22.18143522635943	27.95264117493422	26.468016920584613	23.39790667812174
27	21.920672792274377	27.374176876712696	27.70808716267797	22.997063168334957
28	21.005106912597977	26.969134293913278	30.440423972003938	21.585334821484807
29	22.265836593913527	25.312767953346622	30.474352481946088	21.947042970793767
30	23.294945407851763	26.080022574216517	29.34127462021484	21.28375739771688
31	26.93688541317619	26.144352372770186	25.894339565597495	21.024422648456127
32	27.74159576531885	25.464942359324755	25.770886819025833	21.022575056330563
33	26.675955016170633	26.110087936987032	25.492320315367177	21.72163673147516
34	22.764014616133345	26.428713597186288	28.408744485567368	22.398527301113006
35	22.675498157026855	26.209018097164872	29.090170054058866	22.02531369174941
36	26.896154404953563	27.342515866197374	24.89823546553863	20.86309426331043
37	23.138907854701994	29.5445097540267	26.173325976557415	21.14325641471389
38	23.810003703582396	28.86627548102481	25.365340347464894	21.958380467927903
39	22.83565080172901	28.229108142086552	26.11571469482397	22.819526361360467
40	25.304621751702093	24.93678295579468	27.063361492316957	22.69523380018627
41	21.17584122129199	25.374074419331187	27.52055656193339	25.929527797443434
42	23.35625187383633	26.676122979091137	24.99548599651141	24.972139150561123
43	22.3301663924672	25.92373307668599	27.066720750727068	24.67937978011974
44	22.711610184935573	26.17080653274983	28.035698839124272	23.08188444319032
45	21.758000703764637	28.89541704773254	25.602923898520164	23.743658349982656
46	22.561451334003504	28.298560809715646	26.673015665061783	22.466972191219067
47	22.49132681469239	26.982487346093475	27.33319392410931	23.19299191510482
48	22.587317623761376	26.140405244138304	28.43377096072271	22.838506171377606
49	22.65819797621477	25.934818629439366	28.893905381447993	22.51307801289787
50	21.675027021034836	28.545046395557716	27.380055578930396	22.399871004477053
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	658.0
1	1039.5
2	1421.0
3	5511.5
4	9602.0
5	6988.0
6	4374.0
7	4457.0
8	4540.0
9	4733.0
10	4926.0
11	4952.0
12	4978.0
13	4946.5
14	4915.0
15	4786.5
16	4658.0
17	4492.5
18	4327.0
19	4176.5
20	4026.0
21	4399.5
22	4773.0
23	5217.0
24	5661.0
25	7144.0
26	8627.0
27	9772.5
28	10918.0
29	12723.0
30	14528.0
31	17763.0
32	20998.0
33	23700.5
34	26403.0
35	31332.0
36	36261.0
37	38090.5
38	39920.0
39	45669.5
40	51419.0
41	58673.0
42	65927.0
43	74000.0
44	82073.0
45	82717.5
46	83362.0
47	86178.0
48	88994.0
49	89758.0
50	90522.0
51	85342.5
52	80163.0
53	77154.5
54	74146.0
55	69860.0
56	65574.0
57	63151.0
58	60728.0
59	59133.5
60	57539.0
61	54155.5
62	50772.0
63	44243.0
64	37714.0
65	32292.5
66	26871.0
67	23141.0
68	19411.0
69	16967.5
70	14524.0
71	12477.0
72	10430.0
73	8272.0
74	6114.0
75	4837.0
76	3560.0
77	2941.5
78	2323.0
79	1711.5
80	1100.0
81	791.5
82	483.0
83	377.5
84	272.0
85	190.5
86	109.0
87	94.0
88	79.0
89	45.5
90	12.0
91	7.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1190739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.772994147984893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.05172458910727	20.62905371516312
2	6.627451054181506	3.548734165793155
3	2.503862657234205	2.011077008084678
4	1.389000938761038	1.4875085601997917
5	0.9943188881085347	1.331044688628032
6	0.8014997210974882	1.2875128405532743
7	0.6694439668677412	1.2546113585147676
8	0.5642737665605617	1.208583859998985
9	0.5295935285386917	1.2760923996339355
>10	8.122574154117833	52.17717436320406
>50	0.708353749372552	11.541542688747597
>100	0.036660265198430006	1.5353900005230707
>500	3.106802135393845E-4	0.04791075574455151
>1k	6.21360427078769E-4	0.22666112743733136
>5k	3.106802135393845E-4	0.43710246777364264
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5255	0.4413225736286458	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1407	0.11816191457573826	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	1318	0.11068756461323599	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014780737004498886	0.0
2	0.0	0.0	0.0	0.06449776147417696	0.0
3	0.0	0.0	0.0	0.10212145566744685	0.0
4	0.0	0.0	0.0	0.168046901965922	0.0
5	0.0	0.0	0.0	0.2880564086672226	0.0
6	0.0	0.0	0.0	0.5189214429022649	0.0
7	0.0	0.0	0.0	0.6396028012855882	0.0
8	0.0	0.0	0.0	0.9672984591921487	0.0
9	0.0	0.0	0.0	1.1313142510659346	0.0
10	0.0	0.0	0.0	1.3646147476483093	0.0
11	0.0	0.0	0.0	1.5148575800406303	0.0
12	0.0	0.0	0.0	1.655610507424381	0.0
13	0.0	0.0	0.0	1.7206961391203277	0.0
14	0.0	0.0	0.0	1.7533649271586804	0.0
15	0.0	0.0	0.0	1.7898128809084106	0.0
16	0.0	0.0	0.0	1.8580898080939652	0.0
17	0.0	0.0	0.0	1.9296420122293803	0.0
18	0.0	0.0	0.0	2.0447805942360167	0.0
19	0.0	0.0	0.0	2.0930699338813965	0.0
20	0.0	0.0	0.0	2.1505132526943354	0.0
21	0.0	0.0	0.0	2.2154309214697765	0.0
22	0.0	0.0	0.0	2.281776275069516	0.0
23	0.0	0.0	0.0	2.3636581988160295	0.0
24	0.0	0.0	0.0	2.4299195709555157	0.0
25	0.0	0.0	0.0	2.4878667785299715	0.0
26	0.0	0.0	0.0	2.5467377821672086	0.0
27	0.0	0.0	0.0	2.607120452088997	0.0
28	0.0	0.0	0.0	2.667419140550532	0.0
29	0.0	0.0	0.0	2.7381315300834186	0.0
30	0.0	0.0	0.0	2.8375655790227747	0.0
31	0.0	0.0	0.0	2.9144086151541186	0.0
32	0.0	0.0	0.0	3.0132547938717047	0.0
33	0.0	0.0	0.0	3.0978241243463094	0.0
34	0.0	0.0	0.0	3.1794541037120645	0.0
35	0.0	0.0	0.0	3.279308059952685	0.0
36	0.0	0.0	0.0	3.3684123892809423	0.0
37	0.0	0.0	0.0	3.460456069718049	0.0
38	0.0	0.0	0.0	3.5692960422057225	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGC	20	7.856594E-4	44.000004	12
TATTCGC	20	7.856594E-4	44.000004	12
CGCGTAT	20	7.856594E-4	44.000004	34
CGACGTC	30	2.527795E-6	44.0	18
TAATGCG	60	0.0	44.0	1
ATCGAAT	30	2.527795E-6	44.0	15
CGGTCTA	145	0.0	40.965515	31
CGTTTTT	3695	0.0	40.725307	1
TATAGCG	130	0.0	40.615387	1
TACGGGA	225	0.0	40.08889	4
TCGTACA	55	7.8216544E-11	40.0	34
TACGAAT	195	0.0	39.48718	12
GGCGATA	490	0.0	38.163265	8
GCGATAT	135	0.0	37.481483	9
ATAGCGG	235	0.0	37.44681	2
ATTAGCG	100	0.0	37.399998	1
TAAGGGA	1230	0.0	37.382114	4
GGGCGAT	2525	0.0	37.37822	7
CACGACG	165	0.0	37.333332	26
ATAGGGA	1415	0.0	36.848057	4
>>END_MODULE