FastQCFastQC Report
Thu 26 May 2016
SRR1547199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547199_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2079752
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226631.0896972331316428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT155200.042.199741
TATAGCG850.038.823531
TGATTCG1150.038.2608715
CGTTAGG2700.037.481482
CATATGC14850.037.18518433
GTATACG601.9826984E-1036.6666681
GTTTTTT181300.036.5979042
CGAATAT1750.036.45714614
TACGGGA5150.036.3106774
TATACTA10500.036.24761644
GTTGATC15650.035.84664516
AGGGCGA23150.035.6371466
TCGTTAG1050.035.6190451
GCCAGTA15200.035.60526325
TAGCATA15450.035.59870530
ATAGGGA22300.035.5156974
GGGCGAT47450.035.4689147
ATAGGGC9700.035.3814434
CACGACG2000.035.226
TAGGGAT19700.035.177665
Produced by FastQC (version 0.11.5)