##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547199_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2079752
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.707036223549732	31.0	30.0	31.0	28.0	34.0
2	31.04600404278972	31.0	31.0	33.0	28.0	34.0
3	31.17518579138282	31.0	31.0	34.0	28.0	34.0
4	35.032382707168935	35.0	35.0	37.0	32.0	37.0
5	34.86056510583954	35.0	35.0	37.0	32.0	37.0
6	34.881394993249195	35.0	35.0	37.0	32.0	37.0
7	35.43893887348107	35.0	35.0	37.0	35.0	37.0
8	34.39608616796618	36.0	35.0	37.0	32.0	37.0
9	36.51865799383773	39.0	37.0	39.0	32.0	39.0
10	36.032398574445416	38.0	35.0	39.0	31.0	39.0
11	35.83451440364043	37.0	35.0	39.0	30.0	39.0
12	35.48120016232705	37.0	35.0	39.0	30.0	39.0
13	35.33525679984921	37.0	35.0	39.0	30.0	39.0
14	36.248478183937316	38.0	35.0	40.0	30.0	41.0
15	36.39560630305921	38.0	35.0	40.0	30.0	41.0
16	36.4723890156134	38.0	35.0	40.0	30.0	41.0
17	36.3702917463236	38.0	34.0	40.0	30.0	41.0
18	36.257951909650764	38.0	35.0	40.0	30.0	41.0
19	36.14989527597521	38.0	34.0	40.0	30.0	41.0
20	35.957977201127825	38.0	34.0	40.0	30.0	41.0
21	35.78940469825248	37.0	34.0	40.0	29.0	41.0
22	35.74386128730733	37.0	34.0	40.0	29.0	41.0
23	35.61640811019775	37.0	34.0	40.0	29.0	41.0
24	35.47881141597652	37.0	34.0	40.0	29.0	41.0
25	35.2777182087095	37.0	34.0	40.0	27.0	41.0
26	35.076757709573066	37.0	33.0	40.0	27.0	41.0
27	34.891210105820306	36.0	33.0	40.0	27.0	41.0
28	34.85298920255877	36.0	33.0	40.0	26.0	41.0
29	34.64586691105478	36.0	33.0	40.0	25.0	41.0
30	34.614803591966734	36.0	33.0	40.0	25.0	41.0
31	34.522162257807665	36.0	33.0	40.0	25.0	41.0
32	34.220322423058136	36.0	33.0	40.0	24.0	41.0
33	33.95263281391243	36.0	33.0	40.0	23.0	41.0
34	33.684277259980995	36.0	32.0	40.0	22.0	41.0
35	33.429973381441634	36.0	32.0	40.0	21.0	41.0
36	33.326984659709424	36.0	32.0	40.0	20.0	41.0
37	33.321731629540444	36.0	32.0	40.0	20.0	41.0
38	33.28843006281518	36.0	32.0	40.0	20.0	41.0
39	33.16924902584539	36.0	31.0	40.0	18.0	41.0
40	33.04183623816686	35.0	31.0	40.0	18.0	41.0
41	33.010098800241565	35.0	31.0	40.0	18.0	41.0
42	32.83655022329585	35.0	31.0	39.0	18.0	41.0
43	32.83308827206321	35.0	31.0	39.0	18.0	41.0
44	32.51607210859756	35.0	31.0	39.0	18.0	41.0
45	32.42438569598683	35.0	30.0	39.0	18.0	41.0
46	32.3544441837296	35.0	30.0	39.0	18.0	41.0
47	32.266300982040164	35.0	30.0	39.0	18.0	40.0
48	32.231150637191355	35.0	30.0	39.0	18.0	40.0
49	32.21299029884332	35.0	30.0	39.0	18.0	40.0
50	31.686026987833166	35.0	29.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	4.0
13	13.0
14	48.0
15	119.0
16	292.0
17	889.0
18	1931.0
19	3757.0
20	6296.0
21	9658.0
22	14175.0
23	20275.0
24	28139.0
25	38414.0
26	50519.0
27	59652.0
28	64807.0
29	70179.0
30	80051.0
31	94430.0
32	113359.0
33	137765.0
34	185119.0
35	205882.0
36	195564.0
37	222659.0
38	250595.0
39	225153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.602662240497903	23.57410883605353	26.34400640076317	18.479222522685397
2	35.6346333601314	24.742709707695916	26.274046136270098	13.348610795902587
3	28.516717377841204	24.900180406125347	32.93647511818717	13.646627097846281
4	25.425387257711495	27.27113617392843	32.219418469125166	15.084058099234909
5	22.38843862152795	30.699044886121037	31.991410514330553	14.921105978020458
6	20.969471360046775	39.66924902584539	29.400380429974344	9.960899184133492
7	84.19249025845389	3.7517454004131263	10.409750777977374	1.6460135631556068
8	82.08348880058777	5.931380279956457	10.110099665729376	1.8750312537264058
9	79.11130750204832	5.190138054921933	12.341615731106401	3.3569387119233447
10	40.423810146594406	27.868082348279987	18.47109655381988	13.237010951305733
11	33.265648981224686	24.619233447064843	25.990262300505062	16.124855271205412
12	29.493276121383705	21.144011401359393	30.994608972608273	18.36810350464863
13	24.343671745477348	24.94407987106155	31.783741523027746	18.92850686043336
14	21.1081898226327	27.26137539475861	32.68562790178829	18.944806880820405
15	20.299631879185593	22.472679434855696	37.95745838926949	19.27023029668922
16	23.696334947628372	20.36252399324535	36.06314599048348	19.8779950686428
17	22.960958806626945	20.75353215191042	31.71522373821494	24.570285303247694
18	24.831999199904605	21.640609072620197	32.52065630902146	21.00673541845374
19	27.814662517454003	23.436303943931776	28.534123299316455	20.21491023929776
20	29.36479926452769	22.896912708822974	28.17239747816086	19.565890548488476
21	26.731360277571557	24.10022925810385	29.8164877350761	19.35192272924849
22	25.6030526716647	21.941318003300395	29.4290376929557	23.02659163207921
23	24.19995268666649	24.431711088629797	29.131165638980033	22.237170585723682
24	23.76518931103324	23.18343725598052	32.85132073439526	20.200052698590987
25	24.207693994283932	23.1303540037466	30.902146025102994	21.759805976866474
26	21.881887840473286	25.38201670199139	29.77826202354896	22.95783343398636
27	22.1912516492351	24.46137808738734	30.606389607991723	22.740980655385833
28	21.933143951778867	25.140064776954173	32.441368009262646	20.485423262004314
29	23.28344918047921	23.686574168458545	31.70560720701314	21.3243694440491
30	23.397597405844543	24.200096934634512	31.51846950982617	20.88383614969477
31	26.48729271566995	24.136147002142565	28.701979851443827	20.674580430743667
32	27.227813700864335	23.796154541503025	28.144990364235735	20.831041393396905
33	26.423438948490013	24.211540606764654	27.506068031188335	21.858952413557002
34	23.346245129226947	25.4676519123434	29.21333889809939	21.97276406033027
35	23.6536856317484	25.399663036746688	29.38013763179456	21.56651369971035
36	26.174947782235574	26.568360073701097	26.244282972200534	21.0124091718628
37	24.504796725763455	27.655797422000315	26.677844281433554	21.16156157080267
38	24.473542999357615	27.968671264650784	25.82812758444276	21.72965815154884
39	24.062532455792805	26.847527974489267	25.64084563928776	23.449093930430166
40	25.398893714250548	24.442673934199846	27.48818128315299	22.670251068396617
41	22.39315072181683	24.593124564851962	28.0003096523047	25.013415061026507
42	23.6959502863803	25.83764795033254	25.96564398062846	24.500757782658702
43	23.21195027099385	24.81050625266859	27.629303878539364	24.348239597798198
44	23.217527859090893	25.122562690166905	28.2946235897357	23.365285861006505
45	22.603127680608072	26.85656751381896	27.232381553185185	23.307923252387784
46	23.45976828006416	26.594084294665905	27.46572668279679	22.480420742473143
47	22.80896953098254	26.00300420434744	28.428197208128665	22.759829056541356
48	23.135162269347497	25.055102723786298	29.56833314741373	22.241401859452473
49	23.70715354523039	24.859310148517707	29.065580896183775	22.367955410068124
50	22.292129061541953	26.42685281706665	28.733714404409756	22.54730371698164
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1532.0
1	2569.0
2	3606.0
3	21169.5
4	38733.0
5	27559.0
6	16385.0
7	16465.5
8	16546.0
9	16860.5
10	17175.0
11	17102.0
12	17029.0
13	16618.5
14	16208.0
15	15346.5
16	14485.0
17	13938.5
18	13392.0
19	13048.0
20	12704.0
21	12356.5
22	12009.0
23	12608.0
24	13207.0
25	13557.5
26	13908.0
27	16058.5
28	18209.0
29	20516.5
30	22824.0
31	26614.5
32	30405.0
33	35971.5
34	41538.0
35	46229.0
36	50920.0
37	55579.0
38	60238.0
39	67249.5
40	74261.0
41	85635.0
42	97009.0
43	106293.5
44	115578.0
45	120241.5
46	124905.0
47	127946.5
48	130988.0
49	135719.0
50	140450.0
51	133474.0
52	126498.0
53	121964.0
54	117430.0
55	114454.0
56	111478.0
57	112920.5
58	114363.0
59	112437.0
60	110511.0
61	104798.0
62	99085.0
63	92187.5
64	85290.0
65	72832.0
66	60374.0
67	52584.0
68	44794.0
69	39750.5
70	34707.0
71	29735.0
72	24763.0
73	20003.5
74	15244.0
75	12511.5
76	9779.0
77	7672.0
78	5565.0
79	4274.5
80	2984.0
81	2217.5
82	1451.0
83	1014.0
84	577.0
85	467.5
86	358.0
87	244.5
88	131.0
89	97.0
90	63.0
91	56.5
92	50.0
93	27.5
94	5.0
95	6.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2079752.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.239447797048783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.31107643661115	20.54839402089155
2	5.246470541199685	2.753289797691268
3	2.1750000489955275	1.7121240073259005
4	1.3669277412576233	1.4346971643626882
5	1.004115200094145	1.3173714187546754
6	0.8029196653271173	1.2640901186144866
7	0.7062241372836575	1.2971651968289242
8	0.5989558723384053	1.2573017075967519
9	0.5633509783678169	1.3303816729468823
>10	8.495644995039724	52.0788878198965
>50	0.6772582064697871	11.1578933271765
>100	0.04888433509390012	2.0956351141419303
>500	0.00242552617530254	0.44272070708899874
>1k	5.597368096852016E-4	0.20052902977292256
>5k	0.0	0.0
>10k+	1.865789365617339E-4	1.1095188969100938
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22663	1.0896972331316428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00903953932968931	0.0
2	0.0	0.0	0.0	0.0490443091291654	0.0
3	0.0	0.0	0.0	0.10265647057918444	0.0
4	0.0	0.0	0.0	0.15540314422104173	0.0
5	0.0	0.0	0.0	0.2530109359192827	0.0
6	0.0	0.0	0.0	0.500636614365559	0.0
7	0.0	0.0	0.0	0.637960679927222	0.0
8	0.0	0.0	0.0	0.9211675238201478	0.0
9	0.0	0.0	0.0	1.1070069892948775	0.0
10	0.0	0.0	0.0	1.3612199916143848	0.0
11	0.0	0.0	0.0	1.518209863483723	0.0
12	0.0	0.0	0.0	1.6528413003088829	0.0
13	0.0	0.0	0.0	1.7311679469475207	0.0
14	0.0	0.0	0.0	1.7779042885882548	0.0
15	0.0	0.0	0.0	1.8121391396666526	0.0
16	0.0	0.0	0.0	1.865030061276537	0.0
17	0.0	0.0	0.0	1.9202289503748524	0.0
18	0.0	0.0	0.0	2.008460624151341	0.0
19	0.0	0.0	0.0	2.052360089087545	0.0
20	0.0	0.0	0.0	2.103087291177025	0.0
21	0.0	0.0	0.0	2.1520354349941724	0.0
22	0.0	0.0	0.0	2.20949420892491	0.0
23	0.0	0.0	0.0	2.2730113975127804	0.0
24	0.0	0.0	0.0	2.329123857075267	0.0
25	0.0	0.0	0.0	2.3764852732441177	0.0
26	0.0	0.0	0.0	2.422211879108663	0.0
27	0.0	0.0	0.0	2.4752470486865743	0.0
28	0.0	0.0	0.0	2.527368647800315	0.0
29	0.0	0.0	0.0	2.5878566290596186	0.0
30	0.0	0.0	0.0	2.6717127811392896	0.0
31	0.0	0.0	0.0	2.733354746142809	0.0
32	0.0	0.0	0.0	2.8135085337097885	0.0
33	0.0	0.0	0.0	2.87933368978609	0.0
34	0.0	0.0	0.0	2.9468417388227057	0.0
35	0.0	0.0	0.0	3.0254087987413882	0.0
36	0.0	0.0	0.0	3.101331312579577	0.0
37	0.0	0.0	0.0	3.174416949713235	0.0
38	0.0	0.0	0.0	3.2694282779869908	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15520	0.0	42.19974	1
TATAGCG	85	0.0	38.82353	1
TGATTCG	115	0.0	38.26087	15
CGTTAGG	270	0.0	37.48148	2
CATATGC	1485	0.0	37.185184	33
GTATACG	60	1.9826984E-10	36.666668	1
GTTTTTT	18130	0.0	36.597904	2
CGAATAT	175	0.0	36.457146	14
TACGGGA	515	0.0	36.310677	4
TATACTA	1050	0.0	36.247616	44
GTTGATC	1565	0.0	35.846645	16
AGGGCGA	2315	0.0	35.637146	6
TCGTTAG	105	0.0	35.619045	1
GCCAGTA	1520	0.0	35.605263	25
TAGCATA	1545	0.0	35.598705	30
ATAGGGA	2230	0.0	35.515697	4
GGGCGAT	4745	0.0	35.468914	7
ATAGGGC	970	0.0	35.381443	4
CACGACG	200	0.0	35.2	26
TAGGGAT	1970	0.0	35.17766	5
>>END_MODULE