FastQCFastQC Report
Thu 26 May 2016
SRR1547198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547198_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences198661
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26051.3112790129919814No Hit
AAGATTGGGGTGCTGTTTTTTAAACTTCCTTAAACCTGTTTAGTGCTGTC2290.11527174432827783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA302.5171157E-644.013
CATGCGC254.4295128E-544.036
ACGTTAT254.4295128E-544.013
TTAGCGG502.7284841E-1144.02
CGCGCAT254.4295128E-544.032
CGACCAA207.8407465E-444.029
TGACGTT254.4295128E-544.011
ACGGGCG254.4295128E-544.05
CGTTATG254.4295128E-544.014
TGCGAAG254.4295128E-544.01
CAAGGCG207.8407465E-444.01
GCGGGTG254.4295128E-544.05
AGCGACT302.5171157E-644.019
ATGACGT254.4295128E-544.010
GGGCCGA502.7284841E-1144.07
ATGCGCT254.4295128E-544.037
TTTGCGG207.8407465E-444.02
ATACATC302.5171157E-644.023
CGCTTAA254.4295128E-544.040
CGTTTTT21700.043.391711
Produced by FastQC (version 0.11.5)