##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547198_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	198661
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60744182300502	31.0	30.0	31.0	27.0	34.0
2	30.943733294406048	31.0	31.0	33.0	28.0	34.0
3	31.032336492819425	31.0	31.0	34.0	28.0	34.0
4	34.863299792108165	35.0	35.0	37.0	32.0	37.0
5	34.68279632137158	35.0	35.0	37.0	32.0	37.0
6	34.72822546951843	35.0	35.0	37.0	31.0	37.0
7	35.45945605831039	35.0	35.0	37.0	35.0	37.0
8	34.463664232033466	37.0	35.0	37.0	33.0	37.0
9	36.57206497500768	39.0	37.0	39.0	32.0	39.0
10	35.94222821791897	38.0	35.0	39.0	30.0	39.0
11	35.67229098816577	37.0	35.0	39.0	30.0	39.0
12	35.56266202223889	37.0	35.0	39.0	30.0	39.0
13	35.56082975521114	37.0	35.0	39.0	30.0	39.0
14	36.52192428307519	38.0	35.0	40.0	30.0	41.0
15	36.611685232632475	38.0	35.0	40.0	30.0	41.0
16	36.594636088613264	38.0	35.0	40.0	30.0	41.0
17	36.50691882151001	38.0	35.0	40.0	30.0	41.0
18	36.35008884481604	38.0	35.0	40.0	30.0	41.0
19	36.19909292714725	38.0	35.0	40.0	30.0	41.0
20	35.96665676705543	38.0	34.0	40.0	30.0	41.0
21	35.85757647449675	37.0	34.0	40.0	30.0	41.0
22	35.78542340972813	37.0	34.0	40.0	29.0	41.0
23	35.64606540790593	37.0	34.0	40.0	29.0	41.0
24	35.512048162447584	37.0	34.0	40.0	28.0	41.0
25	35.34401820186146	37.0	34.0	40.0	27.0	41.0
26	35.20655287147452	37.0	34.0	40.0	27.0	41.0
27	34.98012694992978	37.0	33.0	40.0	27.0	41.0
28	34.99634553334575	37.0	33.0	40.0	27.0	41.0
29	34.82238083972194	37.0	33.0	40.0	26.0	41.0
30	34.756182642793505	37.0	33.0	40.0	25.0	41.0
31	34.60081747298161	37.0	33.0	40.0	25.0	41.0
32	34.204176964779194	37.0	33.0	40.0	23.0	41.0
33	33.812212764458046	37.0	33.0	40.0	21.0	41.0
34	33.49791353109065	37.0	32.0	40.0	18.0	41.0
35	33.15717226833651	37.0	31.0	40.0	17.0	41.0
36	33.06829221638872	37.0	31.0	40.0	16.0	41.0
37	33.058773488505544	37.0	31.0	40.0	15.0	41.0
38	33.05576333553138	37.0	31.0	40.0	15.0	41.0
39	32.95135935085397	37.0	31.0	40.0	15.0	41.0
40	32.90764669462048	37.0	31.0	40.0	15.0	41.0
41	32.920970900176684	37.0	31.0	40.0	15.0	41.0
42	32.76967296047035	37.0	31.0	40.0	15.0	41.0
43	32.824917824837286	37.0	31.0	40.0	15.0	41.0
44	32.43190158108537	36.0	30.0	40.0	12.0	41.0
45	32.39623781215236	36.0	30.0	40.0	12.0	41.0
46	32.35351176124151	36.0	30.0	40.0	12.0	41.0
47	32.32535827364203	36.0	30.0	40.0	12.0	41.0
48	32.31199883218145	36.0	30.0	40.0	12.0	41.0
49	32.325015981999485	36.0	30.0	40.0	12.0	41.0
50	31.786057656006967	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	18.0
16	35.0
17	103.0
18	219.0
19	440.0
20	686.0
21	1063.0
22	1483.0
23	2217.0
24	2965.0
25	4247.0
26	5553.0
27	6480.0
28	6265.0
29	6627.0
30	7342.0
31	8601.0
32	9875.0
33	12024.0
34	15203.0
35	17326.0
36	17806.0
37	20864.0
38	25385.0
39	25828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.616784371366293	22.46641263257509	26.232124070653022	21.68467892540559
2	36.72990672552741	23.8290353919491	26.989696014819213	12.45136186770428
3	25.776574164028172	24.630400531558784	37.14317354689648	12.449851757516575
4	23.21744076592789	26.70378181928008	36.396675744106794	13.682101670685237
5	20.38598416397783	30.76245463377311	35.725683450702455	13.125877751546605
6	19.05809393892108	38.89943169519936	32.958658216761215	9.083816149118347
7	83.3847609747258	2.4066122691419047	13.167154096677255	1.0414726594550516
8	81.72313639818586	4.735705548648199	12.510256165024842	1.0309018881411047
9	78.69788232214677	4.997457981184027	14.260977242639472	2.043682454029729
10	32.376259054369	37.18696674233997	20.16651481669779	10.270259386593242
11	22.706016782357885	25.315487186715057	36.73796064652851	15.240535384398548
12	22.966762474768576	23.222474466553575	36.692657340897306	17.11810571778054
13	20.56568727631493	26.13749049889007	37.03041865288104	16.266403571913965
14	18.089106568475945	28.762565375186878	36.0971705568783	17.051157499458878
15	18.17065251861211	25.820367359471664	40.218261259129875	15.790718862786354
16	20.727772436462114	24.25891342538294	39.18937285123904	15.823941286915902
17	20.44588520142353	23.958904868091878	36.13391657144583	19.461293359038763
18	20.681965760768346	24.618823020119702	37.12152863420601	17.577682584905947
19	22.60836299021952	25.164979538006953	34.99227326953957	17.234384202233958
20	23.76359728381514	25.598884531941344	34.22211707380915	16.41540111043436
21	23.057369086030977	24.87000468134158	35.339095242649535	16.7335309899779
22	21.52460724550868	24.02534971635097	35.04965745667242	19.400385581467926
23	19.178399383875046	27.627969254156575	34.85082628195771	18.34280508001067
24	18.82805382032709	25.952250315864717	37.74470077166631	17.47499509214189
25	19.18846678512642	27.540886233332156	35.37886147759248	17.891785503948938
26	19.155244360996875	28.580345412536936	34.28151474119228	17.982895485273907
27	19.119001716491912	26.950433149938842	35.98793925330085	17.942625880268398
28	18.106724520665857	26.29202510809872	37.70241768641051	17.898832684824903
29	18.95943340665757	25.36129386240883	37.186463372277395	18.492809358656203
30	19.805598481835894	26.057958029004187	35.985925773050575	18.150517716109352
31	20.62407820357292	26.90160625386966	34.20449912161924	18.26981642093818
32	21.54826563844942	26.584986484513816	34.02580274940728	17.840945127629478
33	21.152113399207696	27.488032376762423	33.21839716904677	18.141457054983114
34	19.905265754224533	28.268255973744218	33.10866249540675	18.7178157766245
35	19.691333477632753	28.28738403612184	33.06134570952527	18.95993677672014
36	21.854818006553877	28.795787799316425	30.804234348966332	18.54515984516337
37	20.663844438515863	29.497989036600035	31.551738891881143	18.286427633002955
38	20.66082421814045	29.47030368315875	31.034777837622883	18.834094261077915
39	20.485148066303903	29.292614051071926	30.523857224115453	19.698380658508714
40	21.407322020930128	28.144426938352268	31.088638434317755	19.35961260639985
41	19.23830042132074	27.989388959081047	31.171191124579057	21.60111949501915
42	20.563673796064652	28.790250728628163	30.18760602231943	20.458469452987753
43	20.581291748254564	29.13757607180071	29.978204076290766	20.302928103653965
44	20.522397450934	29.637925913994188	30.63912896844373	19.200547666628076
45	20.27775960052552	29.55738670398317	29.939444581472962	20.22540911401835
46	20.50528286880666	29.909242377718826	29.9485052425992	19.63696951087531
47	20.49672557774299	28.535042106905735	30.697016525639153	20.271215789712123
48	20.56568727631493	28.209865046486225	31.092162024755737	20.13228565244311
49	20.48716154655418	27.69944780304136	31.360961638167534	20.452429012236927
50	19.96617353179537	29.027338028098114	30.99199138230453	20.014497057801982
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	156.0
2	224.0
3	2432.5
4	4641.0
5	3464.0
6	2287.0
7	2307.0
8	2327.0
9	2336.0
10	2345.0
11	2337.0
12	2329.0
13	2283.0
14	2237.0
15	2130.5
16	2024.0
17	1944.5
18	1865.0
19	1772.5
20	1680.0
21	1701.5
22	1723.0
23	1783.5
24	1844.0
25	2084.0
26	2324.0
27	2649.0
28	2974.0
29	3435.0
30	3896.0
31	4672.0
32	5448.0
33	6082.0
34	6716.0
35	7550.5
36	8385.0
37	9153.0
38	9921.0
39	10734.0
40	11547.0
41	12432.5
42	13318.0
43	14114.5
44	14911.0
45	15305.0
46	15699.0
47	15192.5
48	14686.0
49	13619.0
50	12552.0
51	12131.0
52	11710.0
53	10746.5
54	9783.0
55	8742.0
56	7701.0
57	7063.5
58	6426.0
59	5954.5
60	5483.0
61	4752.5
62	4022.0
63	3187.0
64	2352.0
65	1819.5
66	1287.0
67	1031.0
68	775.0
69	614.0
70	453.0
71	359.0
72	265.0
73	210.5
74	156.0
75	144.5
76	133.0
77	103.5
78	74.0
79	52.5
80	31.0
81	20.5
82	10.0
83	6.0
84	2.0
85	4.5
86	7.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	198661.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.67060658967414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.13310290068408	38.61144270218454
2	15.610663441459527	17.69331532989523
3	7.587687386487863	12.899965404152594
4	3.9466029734439627	8.94625537894724
5	2.029781615063491	5.7514477685108245
6	1.0638838729792874	3.617456665362484
7	0.555647471873757	2.2042215496773294
8	0.3416985334297061	1.5491410528210792
9	0.1842188467167948	0.9395814409821867
>10	0.5080865040133107	4.563752608637476
>50	0.03169350059341044	1.2505275953667163
>100	0.005942531361264457	0.5107630663302118
>500	0.0	0.0
>1k	9.904218935440762E-4	1.4621294371320899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2605	1.3112790129919814	No Hit
AAGATTGGGGTGCTGTTTTTTAAACTTCCTTAAACCTGTTTAGTGCTGTC	229	0.11527174432827783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01258425156422247	0.0
2	0.0	0.0	0.0	0.04631004575633869	0.0
3	0.0	0.0	0.0	0.12282229526681131	0.0
4	0.0	0.0	0.0	0.17416604164883898	0.0
5	0.0	0.0	0.0	0.27786027453803214	0.0
6	0.0	0.0	0.0	0.547163258012393	0.0
7	0.0	0.0	0.0	0.7052214576590272	0.0
8	0.0	0.0	0.0	1.025364817452847	0.0
9	0.0	0.0	0.0	1.2327532832312331	0.0
10	0.0	0.0	0.0	1.4502091502609973	0.0
11	0.0	0.0	0.0	1.59064939771772	0.0
12	0.0	0.0	0.0	1.7295795349867362	0.0
13	0.0	0.0	0.0	1.8171659258737245	0.0
14	0.0	0.0	0.0	1.8725366327563036	0.0
15	0.0	0.0	0.0	1.914819718012091	0.0
16	0.0	0.0	0.0	1.973714015332652	0.0
17	0.0	0.0	0.0	2.0321049425906446	0.0
18	0.0	0.0	0.0	2.142342986293233	0.0
19	0.0	0.0	0.0	2.1911698823624164	0.0
20	0.0	0.0	0.0	2.251574289870684	0.0
21	0.0	0.0	0.0	2.307448366815832	0.0
22	0.0	0.0	0.0	2.371879734824651	0.0
23	0.0	0.0	0.0	2.451412204710537	0.0
24	0.0	0.0	0.0	2.5128233523439425	0.0
25	0.0	0.0	0.0	2.584805271291295	0.0
26	0.0	0.0	0.0	2.6522568596755276	0.0
27	0.0	0.0	0.0	2.709641046808382	0.0
28	0.0	0.0	0.0	2.7760858950674767	0.0
29	0.0	0.0	0.0	2.8666925063298785	0.0
30	0.0	0.0	0.0	2.972400219469347	0.0
31	0.0	0.0	0.0	3.046898988729544	0.0
32	0.0	0.0	0.0	3.1576404024947022	0.0
33	0.0	0.0	0.0	3.247743643694535	0.0
34	0.0	0.0	0.0	3.3443906957077636	0.0
35	0.0	0.0	0.0	3.457648959785766	0.0
36	0.0	0.0	0.0	3.5537926417364254	0.0
37	0.0	0.0	0.0	3.6433925128736893	0.0
38	0.0	0.0	0.0	3.7465833757003137	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	30	2.5171157E-6	44.0	13
CATGCGC	25	4.4295128E-5	44.0	36
ACGTTAT	25	4.4295128E-5	44.0	13
TTAGCGG	50	2.7284841E-11	44.0	2
CGCGCAT	25	4.4295128E-5	44.0	32
CGACCAA	20	7.8407465E-4	44.0	29
TGACGTT	25	4.4295128E-5	44.0	11
ACGGGCG	25	4.4295128E-5	44.0	5
CGTTATG	25	4.4295128E-5	44.0	14
TGCGAAG	25	4.4295128E-5	44.0	1
CAAGGCG	20	7.8407465E-4	44.0	1
GCGGGTG	25	4.4295128E-5	44.0	5
AGCGACT	30	2.5171157E-6	44.0	19
ATGACGT	25	4.4295128E-5	44.0	10
GGGCCGA	50	2.7284841E-11	44.0	7
ATGCGCT	25	4.4295128E-5	44.0	37
TTTGCGG	20	7.8407465E-4	44.0	2
ATACATC	30	2.5171157E-6	44.0	23
CGCTTAA	25	4.4295128E-5	44.0	40
CGTTTTT	2170	0.0	43.39171	1
>>END_MODULE