FastQCFastQC Report
Thu 26 May 2016
SRR1547197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547197_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4380287
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262800.5999606875074625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT180100.041.1293751
CGGTCTA6050.041.09090431
GGGCGAT86700.037.3264127
TAGGGTA18450.036.8455285
GCGCGAC23300.036.6351939
TCACGAC7000.036.4571425
TACGAAA4450.036.0898920
AGGGCGA45700.035.912476
TAGCATA27450.035.58469830
GCGATAC2850.035.5087749
ATAGGGA52750.035.4502334
ATAGGGC28550.035.4465834
TGGGCGA26300.035.3840336
TAGGGAC37250.035.3771825
GGCGATA19850.035.2443358
ACCCGCT23150.035.16198334
TTAGGGA46550.035.114934
TAACGGG10550.035.0331763
CGACGGT7350.034.7210928
CACGACG7050.034.638326
Produced by FastQC (version 0.11.5)