##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547197_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4380287
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.56881386995875	31.0	30.0	31.0	28.0	33.0
2	30.895522827613807	31.0	31.0	33.0	28.0	34.0
3	31.07136609998386	31.0	31.0	34.0	28.0	34.0
4	34.904950292069906	35.0	35.0	37.0	32.0	37.0
5	34.70610966815645	35.0	35.0	37.0	32.0	37.0
6	34.7336941620492	35.0	35.0	37.0	32.0	37.0
7	35.36451036199226	35.0	35.0	37.0	33.0	37.0
8	34.32100453691733	35.0	35.0	37.0	32.0	37.0
9	36.425523943979016	39.0	37.0	39.0	32.0	39.0
10	35.81123497158976	37.0	35.0	39.0	30.0	39.0
11	35.62059632165655	37.0	35.0	39.0	30.0	39.0
12	35.25242501233367	37.0	34.0	39.0	30.0	39.0
13	35.08654592724175	37.0	34.0	39.0	29.0	39.0
14	35.969141291426794	38.0	34.0	40.0	28.0	41.0
15	36.144766541553096	38.0	34.0	40.0	30.0	41.0
16	36.22031204804617	38.0	34.0	40.0	30.0	41.0
17	36.13922968974408	38.0	34.0	40.0	30.0	41.0
18	36.06117544352687	38.0	34.0	40.0	30.0	41.0
19	36.02564010075139	38.0	34.0	40.0	29.0	41.0
20	35.89397909315075	38.0	34.0	40.0	29.0	41.0
21	35.71269576628198	38.0	34.0	40.0	29.0	41.0
22	35.675625135978535	37.0	34.0	40.0	29.0	41.0
23	35.551307254524644	37.0	34.0	40.0	29.0	41.0
24	35.453061865580956	37.0	34.0	40.0	28.0	41.0
25	35.24371393929211	37.0	33.0	40.0	27.0	41.0
26	35.05266869499647	37.0	33.0	40.0	27.0	41.0
27	34.836542217439174	37.0	33.0	40.0	26.0	41.0
28	34.84170352307965	37.0	33.0	40.0	26.0	41.0
29	34.645412960383645	36.0	33.0	40.0	25.0	41.0
30	34.659080329667894	37.0	33.0	40.0	25.0	41.0
31	34.640581085212	37.0	33.0	40.0	25.0	41.0
32	34.41342405189432	37.0	33.0	40.0	24.0	41.0
33	34.26978049611818	37.0	33.0	40.0	24.0	41.0
34	34.13895116918138	37.0	33.0	40.0	23.0	41.0
35	33.94571337448893	36.0	32.0	40.0	23.0	41.0
36	33.882946026139386	36.0	32.0	40.0	23.0	41.0
37	33.89664626998185	36.0	32.0	40.0	23.0	41.0
38	33.891800240486525	36.0	32.0	40.0	23.0	41.0
39	33.78299093187273	36.0	32.0	40.0	23.0	41.0
40	33.66953055815749	36.0	32.0	40.0	22.0	41.0
41	33.668827636180005	36.0	32.0	40.0	22.0	41.0
42	33.51493475199228	36.0	32.0	40.0	22.0	41.0
43	33.549823561789445	36.0	32.0	40.0	22.0	41.0
44	33.244047022489625	35.0	31.0	39.0	21.0	41.0
45	33.15648175564752	35.0	31.0	39.0	21.0	41.0
46	33.107571718474155	35.0	31.0	39.0	21.0	41.0
47	33.028120303532624	35.0	31.0	39.0	21.0	41.0
48	33.0155033220426	35.0	31.0	39.0	21.0	41.0
49	33.00876129806106	35.0	31.0	39.0	21.0	41.0
50	32.44264565312729	35.0	30.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	7.0
12	8.0
13	18.0
14	69.0
15	242.0
16	679.0
17	1805.0
18	3813.0
19	7442.0
20	12089.0
21	18829.0
22	27525.0
23	38441.0
24	52063.0
25	70605.0
26	91004.0
27	110509.0
28	128086.0
29	146451.0
30	172467.0
31	205718.0
32	244265.0
33	296478.0
34	391540.0
35	433305.0
36	409521.0
37	473047.0
38	541430.0
39	502826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.61560555278684	23.56256564923714	27.808269184188163	15.013559613787864
2	31.75664060368647	25.605057385509216	28.948902206636234	13.689399804168085
3	29.00465197828361	26.06217355164171	31.154396960747093	13.778777509327586
4	25.62192842615107	28.988625631151564	30.298996389962575	15.090449552734785
5	22.56626106919478	32.59610614555621	30.112798544935526	14.724834240313477
6	20.90232900264298	41.633390688783635	27.289673028274176	10.174607280299213
7	88.13748962111386	3.4055074473430618	6.937833068929045	1.519169862614025
8	86.0369651577625	5.674125919146394	6.533818446142912	1.7550904769481999
9	82.89655449517349	5.001635737566968	8.92587631814993	3.1759334491096127
10	39.77116111341562	29.103115846062146	16.073786945923864	15.051936094598368
11	34.11575542881094	25.088538719038272	23.598385219963898	17.197320632186887
12	31.540764338044514	22.0188311861757	27.624925033451003	18.81547944232878
13	24.932909647244575	26.46328882102931	28.6440363382582	19.959765193467916
14	21.286664549605998	29.441632477506612	29.4522253907107	19.81947758217669
15	20.338918431600487	23.389038206857222	36.41966839159169	19.8523749699506
16	23.25571360963334	21.10304644421701	35.04500960781793	20.59623033833171
17	23.196059984197383	21.771678431116502	29.290318191479237	25.741943393206885
18	24.591470832847254	23.32269552200575	31.001050844385308	21.084782800761683
19	28.060764054958042	24.2986133100411	26.631359999926946	21.00926263507391
20	29.062570557591314	24.43677777278064	25.88061010614145	20.620041563486595
21	26.274145963495087	25.98560322645525	27.864201592270092	19.876049217779563
22	25.76422503822238	22.76467272578258	27.421171261152523	24.049930974842514
23	23.65525363977292	26.613256163351856	27.008321600844877	22.72316859603035
24	23.76558887579741	24.146614137384148	31.48042582597898	20.60737116083946
25	24.26936865095826	24.137527974765124	29.52628446492205	22.06681890935457
26	21.947671465362887	27.110757811074937	27.572531206288538	23.36903951727364
27	21.72688684554231	26.803882941916818	28.674742088817467	22.7944881237234
28	21.6367785946446	26.296633074499454	31.123759698850783	20.942828632005163
29	22.67876511287959	24.987974532262385	30.225005804414184	22.10825455044384
30	23.29429555643272	25.40303865933899	29.898292052552723	21.404373731675573
31	26.843378070888964	25.23889416378425	26.58382886783446	21.333898897492336
32	26.916113030949802	25.352151582761586	26.906775743233265	20.82495964305535
33	26.387677337124256	25.318272524151958	25.94291195987843	22.35113817884536
34	22.45215895670763	26.994601038699063	28.012821990887808	22.540418013705494
35	22.866172924285554	25.888349325055643	28.39010320556621	22.855374545092594
36	26.234491027642708	26.942435507079786	25.411348617111162	21.41172484816634
37	23.145903453358194	29.08366506578222	26.306107339541906	21.464324141317682
38	23.62406846857295	29.148227045396798	25.036418846527635	22.191285639502617
39	23.31641739456798	27.66163495679621	25.682015813119097	23.339931835516715
40	24.45634270083216	25.38342807217883	27.394734637250938	22.76549458973807
41	21.772363317746073	25.243756858854226	27.572576865397174	25.411302958002523
42	23.634775529548634	26.190703942458565	25.575401794448627	24.599118733544174
43	22.548248550836966	25.701375275181743	27.396127240064406	24.354248933916885
44	22.575347231813804	26.13582169387531	28.169889324603613	23.11894174970727
45	22.135079276768852	27.994352881443614	26.62460245184847	23.24596538993906
46	23.451865140343546	27.157124635897144	26.818356879355164	22.572653344404145
47	22.76035794001626	26.81819707247493	27.311406763986014	23.110038223522796
48	22.751500072940427	25.90953515146382	28.562329363349935	22.77663541224582
49	22.861447206541488	25.17182093319456	28.87968299794055	23.087048862323407
50	22.10750117515131	27.554518687930724	27.521164709070433	22.816815427847537
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2391.0
1	4008.0
2	5625.0
3	25966.0
4	46307.0
5	33104.5
6	19902.0
7	20025.5
8	20149.0
9	20749.0
10	21349.0
11	21555.0
12	21761.0
13	21509.5
14	21258.0
15	20469.5
16	19681.0
17	19156.0
18	18631.0
19	18077.5
20	17524.0
21	18864.0
22	20204.0
23	21696.5
24	23189.0
25	24576.0
26	25963.0
27	32786.0
28	39609.0
29	46638.5
30	53668.0
31	58783.5
32	63899.0
33	75914.5
34	87930.0
35	102511.0
36	117092.0
37	124612.0
38	132132.0
39	154260.5
40	176389.0
41	209873.5
42	243358.0
43	266769.5
44	290181.0
45	310347.0
46	330513.0
47	328893.5
48	327274.0
49	325873.5
50	324473.0
51	301068.0
52	277663.0
53	268288.5
54	258914.0
55	255919.0
56	252924.0
57	244362.5
58	235801.0
59	230458.0
60	225115.0
61	207503.5
62	189892.0
63	170103.5
64	150315.0
65	127131.5
66	103948.0
67	87600.0
68	71252.0
69	63911.5
70	56571.0
71	46928.5
72	37286.0
73	30390.0
74	23494.0
75	18519.0
76	13544.0
77	10149.0
78	6754.0
79	4991.0
80	3228.0
81	2560.0
82	1892.0
83	1334.5
84	777.0
85	533.0
86	289.0
87	197.5
88	106.0
89	71.0
90	36.0
91	28.5
92	21.0
93	12.5
94	4.0
95	5.0
96	6.0
97	3.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4380287.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.809656266182184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.19761183336055	17.10504048153302
2	6.159112288303106	2.5633801924881285
3	1.934376533164216	1.207611322335495
4	1.0771693067277228	0.8966209205394269
5	0.6580838730918392	0.6847249596679517
6	0.4899895778644789	0.611790881362291
7	0.3727511320642559	0.5429776051761211
8	0.3272180477819978	0.5447436078747642
9	0.2711932812216329	0.5079095067528233
>10	3.784117786311519	19.465011990162186
>50	1.766154001782792	26.866278222492223
>100	0.9554705909450253	27.104376293019783
>500	0.005063810535672921	0.7023672094206043
>1k	0.0015754077222089591	0.5817700523718775
>5k	0.0	0.0
>10k+	1.1252912301492567E-4	0.6153967548032901
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26280	0.5999606875074625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01538711961111224	0.0
2	0.0	0.0	0.0	0.06310088813815168	0.0
3	0.0	0.0	0.0	0.10599762070384885	0.0
4	0.0	0.0	0.0	0.1754222953884072	0.0
5	0.0	0.0	0.0	0.2957340466503679	0.0
6	0.0	0.0	0.0	0.5553973974764668	0.0
7	0.0	0.0	0.0	0.6941097695196684	0.0
8	0.0	0.0	0.0	1.0703636542537054	0.0
9	0.0	0.0	0.0	1.2540274187513285	0.0
10	0.0	0.0	0.0	1.5196721128090465	0.0
11	0.0	0.0	0.0	1.689912099367005	0.0
12	0.0	0.0	0.0	1.8454270233891068	0.0
13	0.0	0.0	0.0	1.924965190637052	0.0
14	0.0	0.0	0.0	1.9657616042053865	0.0
15	0.0	0.0	0.0	2.0053023922861675	2.2829554319157627E-5
16	0.0	0.0	0.0	2.0792244891716	2.2829554319157627E-5
17	0.0	0.0	0.0	2.158054940235651	2.2829554319157627E-5
18	0.0	0.0	0.0	2.2791428963444633	2.2829554319157627E-5
19	0.0	0.0	0.0	2.3319019963760366	2.2829554319157627E-5
20	2.2829554319157627E-5	0.0	0.0	2.393907065906869	2.2829554319157627E-5
21	2.2829554319157627E-5	0.0	0.0	2.4716188688092813	2.2829554319157627E-5
22	2.2829554319157627E-5	0.0	0.0	2.554284684998951	2.2829554319157627E-5
23	4.5659108638315254E-5	0.0	0.0	2.647885857707497	2.2829554319157627E-5
24	4.5659108638315254E-5	0.0	0.0	2.7229722618632066	2.2829554319157627E-5
25	4.5659108638315254E-5	0.0	0.0	2.7900226628985725	2.2829554319157627E-5
26	4.5659108638315254E-5	0.0	0.0	2.857073063933939	2.2829554319157627E-5
27	4.5659108638315254E-5	0.0	0.0	2.927251113911029	2.2829554319157627E-5
28	4.5659108638315254E-5	0.0	0.0	3.003296359348143	2.2829554319157627E-5
29	4.5659108638315254E-5	0.0	0.0	3.0856425617773446	2.2829554319157627E-5
30	4.5659108638315254E-5	0.0	0.0	3.1992424240694732	2.2829554319157627E-5
31	4.5659108638315254E-5	0.0	0.0	3.2935741425162326	2.2829554319157627E-5
32	4.5659108638315254E-5	0.0	0.0	3.3905769188183332	2.2829554319157627E-5
33	4.5659108638315254E-5	0.0	0.0	3.4852282510255606	2.2829554319157627E-5
34	4.5659108638315254E-5	0.0	0.0	3.5870480632890036	2.2829554319157627E-5
35	4.5659108638315254E-5	0.0	0.0	3.700647925581132	2.2829554319157627E-5
36	4.5659108638315254E-5	0.0	0.0	3.803015647148235	2.2829554319157627E-5
37	4.5659108638315254E-5	0.0	0.0	3.912072428130851	2.2829554319157627E-5
38	4.5659108638315254E-5	0.0	0.0	4.03811439752692	2.2829554319157627E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18010	0.0	41.129375	1
CGGTCTA	605	0.0	41.090904	31
GGGCGAT	8670	0.0	37.326412	7
TAGGGTA	1845	0.0	36.845528	5
GCGCGAC	2330	0.0	36.635193	9
TCACGAC	700	0.0	36.45714	25
TACGAAA	445	0.0	36.08989	20
AGGGCGA	4570	0.0	35.91247	6
TAGCATA	2745	0.0	35.584698	30
GCGATAC	285	0.0	35.508774	9
ATAGGGA	5275	0.0	35.450233	4
ATAGGGC	2855	0.0	35.446583	4
TGGGCGA	2630	0.0	35.384033	6
TAGGGAC	3725	0.0	35.377182	5
GGCGATA	1985	0.0	35.244335	8
ACCCGCT	2315	0.0	35.161983	34
TTAGGGA	4655	0.0	35.11493	4
TAACGGG	1055	0.0	35.033176	3
CGACGGT	735	0.0	34.72109	28
CACGACG	705	0.0	34.6383	26
>>END_MODULE