FastQCFastQC Report
Thu 26 May 2016
SRR1547196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547196_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1286513
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62130.48293332442035175No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14300.11115317140207677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG207.856832E-444.01
CACCGTT207.856832E-444.024
TCGACTA207.856832E-444.038
GCGTATT207.856832E-444.043
GTACGAC207.856832E-444.033
AGTACGG1000.041.82
CGTTTTT51250.040.995121
CGGTCTA1700.040.1176531
TCACGAC1750.038.9714325
CGCATCG750.038.1333321
CGTTAGG1850.038.0540542
ACTATCG357.290886E-637.71428713
ACGGGTA1600.037.1255
AGGGCGA14900.037.0604026
GAGTACG601.9826984E-1036.6666641
TCTAACG301.3011818E-436.6666641
TAACGGG3650.036.1643833
TAGGGAC12550.035.9362565
ATAGGGA18750.035.9039964
GGCGATT8650.035.8612758
Produced by FastQC (version 0.11.5)