##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547196_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1286513
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.718245365573452	31.0	30.0	31.0	28.0	33.0
2	31.042545236620228	31.0	31.0	33.0	28.0	34.0
3	31.208641498375844	31.0	31.0	34.0	28.0	34.0
4	35.05484670578533	35.0	35.0	37.0	32.0	37.0
5	34.87467985166104	35.0	35.0	37.0	32.0	37.0
6	34.911383717070876	35.0	35.0	37.0	32.0	37.0
7	35.45641746332917	35.0	35.0	37.0	35.0	37.0
8	34.40720536830953	36.0	35.0	37.0	33.0	37.0
9	36.538586862316976	39.0	37.0	39.0	32.0	39.0
10	36.02645678667841	38.0	35.0	39.0	31.0	39.0
11	35.852820764345175	37.0	35.0	39.0	30.0	39.0
12	35.47507565022662	37.0	35.0	39.0	30.0	39.0
13	35.306990290809345	37.0	35.0	39.0	30.0	39.0
14	36.22751033219252	38.0	35.0	40.0	30.0	41.0
15	36.39203568094531	38.0	35.0	40.0	30.0	41.0
16	36.45995104596689	38.0	35.0	40.0	31.0	41.0
17	36.374764965453124	38.0	34.0	40.0	30.0	41.0
18	36.306114279451506	38.0	35.0	40.0	30.0	41.0
19	36.28877827118731	38.0	35.0	40.0	30.0	41.0
20	36.14891260329278	38.0	34.0	40.0	30.0	41.0
21	35.98637946138127	38.0	34.0	40.0	30.0	41.0
22	35.930073773059426	38.0	34.0	40.0	30.0	41.0
23	35.813677747523734	38.0	34.0	40.0	29.0	41.0
24	35.74079313617507	38.0	34.0	40.0	29.0	41.0
25	35.52875408176987	37.0	34.0	40.0	29.0	41.0
26	35.36029328891352	37.0	34.0	40.0	27.0	41.0
27	35.16497928897726	37.0	33.0	40.0	27.0	41.0
28	35.16483238024023	37.0	33.0	40.0	27.0	41.0
29	35.02118594992822	37.0	33.0	40.0	27.0	41.0
30	35.010480267202894	37.0	33.0	40.0	27.0	41.0
31	34.97173367078296	37.0	33.0	40.0	26.0	41.0
32	34.75091817960642	37.0	33.0	40.0	25.0	41.0
33	34.60531452072385	37.0	33.0	40.0	25.0	41.0
34	34.46106957333505	37.0	33.0	40.0	24.0	41.0
35	34.24434420794815	37.0	33.0	40.0	24.0	41.0
36	34.1990722208015	37.0	33.0	40.0	23.0	41.0
37	34.19154567423726	37.0	33.0	40.0	23.0	41.0
38	34.198733320222956	37.0	33.0	40.0	23.0	41.0
39	34.095317342304355	37.0	33.0	40.0	23.0	41.0
40	34.004076134481345	36.0	33.0	40.0	23.0	41.0
41	34.0045277428211	36.0	33.0	40.0	23.0	41.0
42	33.84754060005612	36.0	32.0	40.0	23.0	41.0
43	33.86859674173522	36.0	33.0	40.0	23.0	41.0
44	33.566909934062075	36.0	32.0	40.0	23.0	41.0
45	33.48603239920622	35.0	32.0	39.0	23.0	41.0
46	33.422284112169876	35.0	32.0	39.0	23.0	41.0
47	33.332570288835015	35.0	32.0	39.0	22.0	41.0
48	33.33108721015645	35.0	32.0	39.0	22.0	41.0
49	33.332367414864834	35.0	32.0	39.0	22.0	41.0
50	32.75439268783137	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	4.0
14	19.0
15	61.0
16	136.0
17	363.0
18	888.0
19	1631.0
20	2933.0
21	4528.0
22	6836.0
23	9797.0
24	13538.0
25	18265.0
26	23908.0
27	29189.0
28	33224.0
29	39066.0
30	47062.0
31	56494.0
32	69218.0
33	86076.0
34	117618.0
35	132423.0
36	123933.0
37	144961.0
38	167817.0
39	156520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.83611358765904	23.573411228646737	26.926117341993432	15.664357841700783
2	31.99252553219439	25.975407943798466	28.76760670121483	13.264459822792308
3	29.701992906406698	26.12970098242303	30.71791734712358	13.450388764046691
4	25.879101105080167	28.75050621330682	30.19402058121449	15.176372100398519
5	23.038165957125965	32.431386235506366	30.01811874423344	14.512329063134224
6	21.127108703915155	41.565145474627926	26.975708756926668	10.332037064530246
7	88.7076150804539	3.3882284905010676	6.454345972407586	1.4498104566374377
8	86.61428217204178	5.669433577429843	6.234371514318161	1.4819127362102054
9	83.412915376681	5.469901975339542	8.080058266026072	3.037124381953389
10	38.59634531481609	33.07724057199577	14.935488409367025	13.390925703821106
11	34.33933430909754	25.750536527808116	23.867384161683557	16.04274500141079
12	31.195798254662023	22.47812497813858	28.61549008832402	17.71058667887538
13	24.487898684272917	28.44479612720587	28.99006850299997	18.07723668552125
14	21.614161691331528	30.99230244855668	29.31715419898594	18.076381661125847
15	20.679853215630157	23.97154167894145	36.40064266742738	18.947962438001014
16	23.29754926689431	22.611197865859108	34.77151027622729	19.31974259101929
17	23.018655855012735	21.958192416244533	29.196440300253474	25.826711428489258
18	24.706940388476447	22.98655357543997	31.614138372484383	20.6923676635992
19	28.909152103398878	24.818948584273926	26.106459864766236	20.165439447560964
20	29.763399203894558	24.923961125927217	25.697447285802788	19.61519238437544
21	27.046287134292463	25.706774824661704	27.729140708255574	19.517797332790263
22	25.382254201861933	23.25005654820433	27.213172350376563	24.154516899557173
23	23.155615217257814	27.441930240891466	27.179826398955935	22.222628142894788
24	23.039798276426275	25.12893379235188	31.97876741237749	19.852500518844348
25	23.08021761148158	25.65337466469441	30.06973112591944	21.196676597904567
26	21.672847456652207	28.5906943808574	27.116865511658254	22.619592650832132
27	21.86771528931305	27.313443393109903	28.884434125422754	21.934407192154296
28	20.769397588675744	26.86517742144852	31.78382185022615	20.58160313964958
29	22.36432900405981	25.22632884393706	31.16338505712729	21.245957094875838
30	22.89615417799898	26.14415866765435	30.327637575368456	20.632049578978215
31	26.860513652019062	25.791966346239796	26.52347858125025	20.824041420490893
32	27.085074150047454	25.872416368897944	26.696271238611658	20.346238242442944
33	26.859891816095132	25.52496554640334	26.041944387658734	21.573198249842793
34	22.819046523431943	26.912514681157518	28.0367940316188	22.23164476379174
35	22.79378443902238	26.611235176014546	29.208332912298594	21.38664747266448
36	26.949125271178758	26.93567806932382	25.60409416772314	20.511102491774277
37	23.752111327285462	29.501062173487558	26.500937029007872	20.24588947021911
38	23.62696684759501	29.75686992669332	25.276930742246677	21.339232483464997
39	23.38250760000093	28.644405458786657	25.774710399350802	22.198376541861606
40	24.881443094628658	26.108791749480964	26.952934016212815	22.056831139677563
41	21.679454463343937	25.714625503201287	27.53124142546558	25.074678607989192
42	23.362297932473282	26.63743001431	25.255632861852156	24.744639191364566
43	22.98258937142493	25.850574382069986	26.835717944552446	24.331118301952642
44	22.03164678475849	27.108315267704253	28.66088411077074	22.199153836766516
45	22.068101915798753	29.326870385297312	26.070237922197443	22.534789776706493
46	23.210725426015905	28.61642284220991	26.70660926084696	21.466242470927227
47	22.21423335792176	27.41589086157699	27.78557231835201	22.584303462149236
48	22.926468679290455	26.225930091650838	29.161462029532544	21.68613919952616
49	22.68480769335405	25.912136138538827	29.515986235661824	21.8870699324453
50	22.06545911312206	28.315298796047923	27.766139945729268	21.85310214510075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	776.0
1	1096.5
2	1417.0
3	6634.5
4	11852.0
5	8782.5
6	5713.0
7	5764.0
8	5815.0
9	6047.5
10	6280.0
11	6363.5
12	6447.0
13	6496.5
14	6546.0
15	6255.5
16	5965.0
17	5774.5
18	5584.0
19	5894.0
20	6204.0
21	6044.5
22	5885.0
23	6646.0
24	7407.0
25	8848.0
26	10289.0
27	12127.5
28	13966.0
29	15713.0
30	17460.0
31	21335.5
32	25211.0
33	28686.5
34	32162.0
35	35800.0
36	39438.0
37	42252.5
38	45067.0
39	51191.0
40	57315.0
41	66385.0
42	75455.0
43	81383.0
44	87311.0
45	87882.0
46	88453.0
47	89340.0
48	90227.0
49	90341.0
50	90455.0
51	89365.0
52	88275.0
53	83633.0
54	78991.0
55	74276.0
56	69561.0
57	66155.5
58	62750.0
59	59308.0
60	55866.0
61	52239.0
62	48612.0
63	44623.0
64	40634.0
65	34882.0
66	29130.0
67	24333.5
68	19537.0
69	17298.0
70	15059.0
71	13481.5
72	11904.0
73	9945.5
74	7987.0
75	6334.0
76	4681.0
77	3564.0
78	2447.0
79	1896.5
80	1346.0
81	1005.5
82	665.0
83	452.5
84	240.0
85	156.5
86	73.0
87	56.0
88	39.0
89	23.5
90	8.0
91	6.5
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1286513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.62524359762427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.42238764153846	20.347646872680322
2	6.557057278101464	3.4916649462605323
3	2.664812613369383	2.1285385491894466
4	1.5097784684468893	1.6079287800338606
5	1.0438350331782458	1.3896181017052502
6	0.8420909822521725	1.3452526520296075
7	0.720575660203036	1.3429851738397336
8	0.6216958859371879	1.3242243525374766
9	0.5753307175644522	1.3786488453914556
>10	8.366329451420851	53.021540605989784
>50	0.6403554564099223	10.450603551646134
>100	0.034597559592352875	1.4666537033683762
>500	5.766259928173865E-4	0.11798632581798113
>1k	2.883129964086932E-4	0.10977257379291673
>5k	2.883129964086932E-4	0.47693496571705707
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6213	0.48293332442035175	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1430	0.11115317140207677	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011659423573644417	0.0
2	0.0	0.0	0.0	0.04827001359488789	0.0
3	0.0	0.0	0.0	0.08006137520569166	0.0
4	0.0	0.0	0.0	0.11993660382755557	0.0
5	0.0	0.0	0.0	0.1810319833534523	0.0
6	0.0	0.0	0.0	0.33027260509610085	0.0
7	0.0	0.0	0.0	0.4007732529714041	0.0
8	0.0	0.0	0.0	0.6009266909856332	0.0
9	0.0	0.0	0.0	0.7117689444257462	0.0
10	0.0	0.0	0.0	0.8756227103806957	0.0
11	0.0	0.0	0.0	0.9893409549689742	0.0
12	0.0	0.0	0.0	1.0938093901888282	0.0
13	0.0	0.0	0.0	1.1522619670380323	0.0
14	0.0	0.0	0.0	1.1835092222153993	0.0
15	0.0	0.0	0.0	1.2137459940163837	0.0
16	0.0	0.0	0.0	1.2638815153830547	0.0
17	0.0	0.0	0.0	1.314017036749726	0.0
18	0.0	0.0	0.0	1.3943116004268903	0.0
19	0.0	0.0	0.0	1.4309221904481337	0.0
20	0.0	0.0	0.0	1.4731293037847266	0.0
21	0.0	0.0	0.0	1.52427530852778	0.0
22	0.0	0.0	0.0	1.5756545017423065	0.0
23	0.0	0.0	0.0	1.6390817659829322	0.0
24	0.0	0.0	0.0	1.6924041964597327	0.0
25	0.0	0.0	0.0	1.737875948396946	0.0
26	0.0	0.0	0.0	1.7824149464482675	0.0
27	0.0	0.0	0.0	1.833871869153285	0.0
28	0.0	0.0	0.0	1.8851733328773204	0.0
29	0.0	0.0	0.0	1.9327437810577894	0.0
30	0.0	0.0	0.0	2.0107064600202254	0.0
31	0.0	0.0	0.0	2.067293529097646	0.0
32	0.0	0.0	0.0	2.1319644651861274	0.0
33	0.0	0.0	0.0	2.1905725010163133	0.0
34	0.0	0.0	0.0	2.2501132907323904	0.0
35	0.0	0.0	0.0	2.320225291155239	0.0
36	0.0	0.0	0.0	2.384274391319792	0.0
37	0.0	0.0	0.0	2.4502667287466195	0.0
38	0.0	0.0	0.0	2.5255866050323625	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	20	7.856832E-4	44.0	1
CACCGTT	20	7.856832E-4	44.0	24
TCGACTA	20	7.856832E-4	44.0	38
GCGTATT	20	7.856832E-4	44.0	43
GTACGAC	20	7.856832E-4	44.0	33
AGTACGG	100	0.0	41.8	2
CGTTTTT	5125	0.0	40.99512	1
CGGTCTA	170	0.0	40.11765	31
TCACGAC	175	0.0	38.97143	25
CGCATCG	75	0.0	38.13333	21
CGTTAGG	185	0.0	38.054054	2
ACTATCG	35	7.290886E-6	37.714287	13
ACGGGTA	160	0.0	37.125	5
AGGGCGA	1490	0.0	37.060402	6
GAGTACG	60	1.9826984E-10	36.666664	1
TCTAACG	30	1.3011818E-4	36.666664	1
TAACGGG	365	0.0	36.164383	3
TAGGGAC	1255	0.0	35.936256	5
ATAGGGA	1875	0.0	35.903996	4
GGCGATT	865	0.0	35.861275	8
>>END_MODULE