FastQCFastQC Report
Thu 26 May 2016
SRR1547195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547195_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3192580
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122620.3840780810504357No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA66930.20964235821811827No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA44410.13910379692912944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82900.041.3196641
ATCGTAC800.041.2527
CGTACGT404.1276144E-738.532
TATACGG3400.038.176472
TAATGCG1450.037.9310341
TATACTA26450.037.92816544
TCACGAC6400.037.812525
TAAGGGA31750.037.348034
AGGGCGA35950.037.3296246
TAGCATA19550.037.24808530
GGGCGAT65850.037.184517
GTTGATC20550.037.1484216
ATAGGGC15400.037.04
CGACGGT6500.036.89230728
CTCACGA6650.036.72180624
TATAGCG1500.036.6666681
CTATCGA301.3016137E-436.66666430
CGTAATC301.3016137E-436.66666434
CGGTCTA6450.036.49612431
GCTACGA6600.036.33333210
Produced by FastQC (version 0.11.5)