##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547195_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3192580
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.522067105601113	31.0	30.0	31.0	27.0	33.0
2	30.848192371060396	31.0	31.0	33.0	28.0	34.0
3	31.039793521227345	31.0	31.0	34.0	28.0	34.0
4	34.87450244003283	35.0	35.0	37.0	32.0	37.0
5	34.64693977911282	35.0	35.0	37.0	31.0	37.0
6	34.67696001353138	35.0	35.0	37.0	31.0	37.0
7	35.32315775955497	35.0	35.0	37.0	33.0	37.0
8	34.29184765925991	35.0	35.0	37.0	32.0	37.0
9	36.390019983837526	39.0	37.0	39.0	32.0	39.0
10	35.797614155322655	37.0	35.0	39.0	30.0	39.0
11	35.54013399820835	37.0	35.0	39.0	30.0	39.0
12	34.94009233911132	37.0	33.0	39.0	29.0	39.0
13	34.61301110700437	37.0	33.0	39.0	27.0	39.0
14	35.4459242994694	38.0	33.0	40.0	27.0	41.0
15	35.74359076358306	38.0	33.0	40.0	29.0	41.0
16	35.89695168171197	38.0	34.0	40.0	29.0	41.0
17	35.81937931077687	38.0	34.0	40.0	29.0	41.0
18	35.77207368335328	37.0	34.0	40.0	29.0	41.0
19	35.750848530029	37.0	34.0	40.0	29.0	41.0
20	35.61022746493432	37.0	34.0	40.0	29.0	41.0
21	35.41696308314905	37.0	34.0	40.0	28.0	41.0
22	35.36601839264795	37.0	33.0	40.0	28.0	41.0
23	35.28807829404432	37.0	34.0	40.0	27.0	41.0
24	35.19503066485413	37.0	33.0	40.0	27.0	41.0
25	34.96870744037737	36.0	33.0	40.0	27.0	41.0
26	34.77092570898772	36.0	33.0	40.0	26.0	41.0
27	34.54299062200477	36.0	33.0	40.0	25.0	41.0
28	34.572667247179396	36.0	33.0	40.0	25.0	41.0
29	34.42644475627862	36.0	33.0	40.0	25.0	41.0
30	34.43437815183958	36.0	33.0	39.0	25.0	41.0
31	34.391312042298075	36.0	33.0	40.0	25.0	41.0
32	34.17867618039329	36.0	33.0	40.0	24.0	41.0
33	34.05136691954469	36.0	32.0	40.0	24.0	41.0
34	33.961844965513784	36.0	32.0	40.0	23.0	41.0
35	33.77142373879433	36.0	32.0	40.0	23.0	41.0
36	33.68095709426232	36.0	32.0	40.0	23.0	41.0
37	33.66967436994531	36.0	32.0	40.0	23.0	41.0
38	33.6997121450363	35.0	32.0	40.0	23.0	41.0
39	33.61479242493532	35.0	32.0	40.0	23.0	41.0
40	33.48177242230422	35.0	32.0	39.0	23.0	41.0
41	33.515929123154315	35.0	32.0	39.0	23.0	41.0
42	33.35262483633926	35.0	32.0	39.0	22.0	41.0
43	33.378995984438916	35.0	32.0	39.0	23.0	41.0
44	32.960234355912775	35.0	31.0	39.0	21.0	41.0
45	32.903152309417464	35.0	31.0	39.0	21.0	41.0
46	32.8610001942003	35.0	31.0	39.0	21.0	40.0
47	32.809916431224906	35.0	31.0	39.0	21.0	40.0
48	32.838002493281294	35.0	31.0	39.0	21.0	40.0
49	32.84203089664159	35.0	31.0	39.0	21.0	40.0
50	32.251451803870225	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	9.0
13	24.0
14	80.0
15	190.0
16	631.0
17	1429.0
18	3157.0
19	5755.0
20	9530.0
21	14528.0
22	21490.0
23	29601.0
24	40137.0
25	53199.0
26	68037.0
27	82411.0
28	96190.0
29	111965.0
30	133004.0
31	158282.0
32	188165.0
33	227677.0
34	311401.0
35	341202.0
36	282499.0
37	321036.0
38	362320.0
39	328626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.434463662617695	23.902361099800164	28.88867311077561	13.774502126806532
2	29.407407175387927	26.30377312393112	30.65107217360254	13.637747527078414
3	28.358662899598443	26.08229081181991	31.38439757186977	14.174648716711877
4	25.779808180217877	29.105738932148924	29.791109384886205	15.323343502746994
5	22.384497804283683	33.3458519441956	29.31215505954432	14.957495191976394
6	21.008901891260358	42.5789486872686	26.085767623677402	10.326381797793633
7	88.78580959600073	3.9054933627348416	5.833181940624824	1.4755151006396081
8	86.63626283444738	6.0998941295128075	5.399645427835794	1.864197608204023
9	83.79270057445703	5.597134605867355	7.442914508015461	3.1672503116601622
10	44.6004172174229	29.544725582444293	13.302250844144861	12.552606355987947
11	38.76018768519505	23.44671081069229	21.854299657330433	15.938801846782228
12	35.348088379930964	20.77589285154953	25.921668368529527	17.954350399989977
13	24.18683321952778	30.765086544424886	26.86319528406493	18.184884951982408
14	20.35667078037199	33.484423256425835	28.09182542019307	18.067080543009105
15	19.21164074197044	22.980786699158674	39.28405866102024	18.52351389785064
16	21.518395780215375	19.709545258067145	37.19747038445395	21.57458857726353
17	21.370114452887634	20.473316252059462	28.239730875968654	29.91683841908425
18	24.018442764159396	21.85868482543899	32.26760175156143	21.855270658840187
19	28.698043588570997	24.56029292922965	25.17239975192477	21.569263730274574
20	29.99724360861748	24.00074547857845	24.65491859248633	21.34709232031774
21	26.19204530505109	26.02036597360129	26.732204048136616	21.05538467321101
22	25.304518602509567	23.074504006164293	25.29267864861648	26.32829874270966
23	22.483352022502174	27.003144791986415	25.630493206121695	24.88300997938971
24	22.99149903839528	23.220216877885598	33.33658044590895	20.451703637810173
25	22.5447443760219	24.565837034624035	29.961567133791476	22.927851455562585
26	20.97190986600179	28.699641042667682	25.889030188750162	24.43941890258036
27	21.252717238095833	27.804315005418818	27.504901991492776	23.438065764992576
28	20.02634233128066	26.287485356670782	32.031804997838734	21.654367314209825
29	20.943719499589676	24.01036152578792	31.247924875805776	23.79799409881663
30	22.64622969510553	26.554103577670723	29.596971728194752	21.202694999029
31	28.306667334882757	25.48117823202551	24.90725369450413	21.3049007385876
32	28.826998853591768	24.16033427510039	25.102706901628153	21.909959969679697
33	27.68187484730218	24.927738694096938	24.67640591621823	22.71398054238265
34	21.978525205319833	25.59779238108364	27.649299312781512	24.774383100815015
35	22.129061761960546	25.049427109109246	29.174742684599913	23.64676844433029
36	28.485832774746445	24.94061229475847	25.393161643561008	21.18039328693408
37	22.601594948286337	29.532384466481652	26.370866195991955	21.49515438924005
38	23.173671450676256	29.311810510621505	24.64129324872047	22.87322478998177
39	22.7707058241297	28.326557204517975	24.923165590212303	23.97957138114002
40	25.055002537132975	23.76316333498299	26.852921461639173	24.328912666244857
41	21.176916475076585	23.82941069605147	27.002455694140785	27.991217134731155
42	22.76337632886255	24.325122628093894	25.037900381509626	27.87360066153393
43	22.279692286489297	24.621967186413496	26.828615101266063	26.269725425831147
44	21.6457849137688	25.885240150599202	28.81089902210751	23.658075913524485
45	21.076527448020098	29.687713385412422	25.426113049633837	23.80964611693364
46	23.07531839452731	28.202550914934005	27.005431343928738	21.716699346609953
47	22.172756829899328	26.301423926730106	27.13382280162126	24.391996441749306
48	22.40351064029719	24.893690995996966	29.355505578560287	23.347292785145555
49	22.508535416497004	23.35919538429734	30.50795908011702	23.624310119088637
50	21.69333266511724	28.021975956749714	27.270765337125457	23.013926041007586
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1940.0
1	3155.5
2	4371.0
3	13178.0
4	21985.0
5	16193.0
6	10401.0
7	10803.0
8	11205.0
9	11880.5
10	12556.0
11	12464.0
12	12372.0
13	12390.5
14	12409.0
15	12139.5
16	11870.0
17	11219.0
18	10568.0
19	10662.5
20	10757.0
21	11589.5
22	12422.0
23	12334.5
24	12247.0
25	15427.0
26	18607.0
27	21346.5
28	24086.0
29	28443.5
30	32801.0
31	39088.0
32	45375.0
33	53176.5
34	60978.0
35	72697.0
36	84416.0
37	90992.5
38	97569.0
39	114383.0
40	131197.0
41	163102.0
42	195007.0
43	217493.5
44	239980.0
45	242530.0
46	245080.0
47	246915.5
48	248751.0
49	249903.0
50	251055.0
51	230219.0
52	209383.0
53	196966.0
54	184549.0
55	180802.5
56	177056.0
57	174775.5
58	172495.0
59	163329.5
60	154164.0
61	143001.5
62	131839.0
63	119597.0
64	107355.0
65	91967.0
66	76579.0
67	65317.5
68	54056.0
69	47031.0
70	40006.0
71	33173.0
72	26340.0
73	21818.5
74	17297.0
75	13880.5
76	10464.0
77	8089.5
78	5715.0
79	4177.5
80	2640.0
81	2018.5
82	1397.0
83	1099.0
84	801.0
85	535.0
86	269.0
87	182.0
88	95.0
89	74.0
90	53.0
91	32.0
92	11.0
93	10.0
94	9.0
95	5.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3192580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.574875321188603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.00755750824288	17.0787014644849
2	6.673600246993922	2.746169860507068
3	2.0438242278710734	1.261542860006157
4	1.0081618640834626	0.8297121862837733
5	0.6069225259109765	0.6243677650119601
6	0.4561331228543109	0.563092927955511
7	0.3314037236140334	0.47730132060354696
8	0.2734236364040421	0.45005257831033385
9	0.2242226504792721	0.4152017770017726
>10	2.7955574995972508	14.149697769482794
>50	1.3103261983417775	19.774955875375298
>100	1.2604223442482076	39.50255058576227
>500	0.006255149154670211	0.8698759595420057
>1k	0.0018765447464010635	0.646905091084168
>5k	1.563787288667553E-4	0.21534545780495057
>10k+	1.563787288667553E-4	0.39452652078355066
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12262	0.3840780810504357	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6693	0.20964235821811827	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	4441	0.13910379692912944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026217040763269833	0.0
2	3.132262934679789E-5	0.0	0.0	0.10483684042373254	0.0
3	3.132262934679789E-5	0.0	0.0	0.15792869716655494	0.0
4	3.132262934679789E-5	0.0	0.0	0.2664616078532096	0.0
5	3.132262934679789E-5	3.132262934679789E-5	0.0	0.4387987145192916	0.0
6	3.132262934679789E-5	3.132262934679789E-5	0.0	0.72405389998058	0.0
7	3.132262934679789E-5	3.132262934679789E-5	0.0	0.8765951048994857	0.0
8	3.132262934679789E-5	3.132262934679789E-5	0.0	1.3240075424891466	0.0
9	3.132262934679789E-5	3.132262934679789E-5	0.0	1.5129456427090315	0.0
10	3.132262934679789E-5	3.132262934679789E-5	0.0	1.8062507439124469	0.0
11	3.132262934679789E-5	3.132262934679789E-5	0.0	2.0160183926479522	0.0
12	3.132262934679789E-5	3.132262934679789E-5	0.0	2.2083080142079448	0.0
13	6.264525869359578E-5	3.132262934679789E-5	0.0	2.292565887150831	0.0
14	6.264525869359578E-5	3.132262934679789E-5	0.0	2.3291507182278908	0.0
15	6.264525869359578E-5	3.132262934679789E-5	0.0	2.3720940430623507	0.0
16	6.264525869359578E-5	3.132262934679789E-5	0.0	2.4654668011451553	0.0
17	6.264525869359578E-5	3.132262934679789E-5	0.0	2.5654173113907874	0.0
18	6.264525869359578E-5	3.132262934679789E-5	0.0	2.7335571857243983	0.0
19	6.264525869359578E-5	3.132262934679789E-5	0.0	2.7946363129506544	0.0
20	6.264525869359578E-5	3.132262934679789E-5	0.0	2.8685890408384442	0.0
21	6.264525869359578E-5	3.132262934679789E-5	0.0	2.960990797411498	0.0
22	6.264525869359578E-5	3.132262934679789E-5	0.0	3.062945955935325	0.0
23	6.264525869359578E-5	3.132262934679789E-5	0.0	3.177179585163097	0.0
24	6.264525869359578E-5	3.132262934679789E-5	0.0	3.2655720451797605	0.0
25	6.264525869359578E-5	3.132262934679789E-5	0.0	3.34030783880122	0.0
26	6.264525869359578E-5	3.132262934679789E-5	0.0	3.4145424703531315	0.0
27	6.264525869359578E-5	3.132262934679789E-5	0.0	3.4916587838049478	0.0
28	6.264525869359578E-5	3.132262934679789E-5	0.0	3.5703725513534508	0.0
29	6.264525869359578E-5	3.132262934679789E-5	0.0	3.653690745415933	0.0
30	6.264525869359578E-5	3.132262934679789E-5	0.0	3.7797956511661415	0.0
31	6.264525869359578E-5	3.132262934679789E-5	0.0	3.878712514643329	0.0
32	6.264525869359578E-5	3.132262934679789E-5	0.0	3.969548139749043	0.0
33	6.264525869359578E-5	3.132262934679789E-5	0.0	4.071284039867443	0.0
34	6.264525869359578E-5	3.132262934679789E-5	0.0	4.1736463925727785	0.0
35	6.264525869359578E-5	3.132262934679789E-5	0.0	4.301505365566407	0.0
36	6.264525869359578E-5	3.132262934679789E-5	0.0	4.40408697667717	0.0
37	6.264525869359578E-5	3.132262934679789E-5	0.0	4.508297364513966	0.0
38	6.264525869359578E-5	3.132262934679789E-5	0.0	4.6348407870750306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8290	0.0	41.319664	1
ATCGTAC	80	0.0	41.25	27
CGTACGT	40	4.1276144E-7	38.5	32
TATACGG	340	0.0	38.17647	2
TAATGCG	145	0.0	37.931034	1
TATACTA	2645	0.0	37.928165	44
TCACGAC	640	0.0	37.8125	25
TAAGGGA	3175	0.0	37.34803	4
AGGGCGA	3595	0.0	37.329624	6
TAGCATA	1955	0.0	37.248085	30
GGGCGAT	6585	0.0	37.18451	7
GTTGATC	2055	0.0	37.14842	16
ATAGGGC	1540	0.0	37.0	4
CGACGGT	650	0.0	36.892307	28
CTCACGA	665	0.0	36.721806	24
TATAGCG	150	0.0	36.666668	1
CTATCGA	30	1.3016137E-4	36.666664	30
CGTAATC	30	1.3016137E-4	36.666664	34
CGGTCTA	645	0.0	36.496124	31
GCTACGA	660	0.0	36.333332	10
>>END_MODULE