FastQCFastQC Report
Thu 26 May 2016
SRR1547194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547194_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences710626
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29540.4156898284048149No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12460.17533836363994562No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA7920.11145103049986912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.854451E-444.01
CTATGCG351.4450416E-744.01
GTATACG207.854451E-444.01
CGGTCTA302.526347E-644.031
CGTTTTT24500.041.4857141
ACGTAGG1400.040.8571432
TAAGGGA7900.039.8227844
TGCGTAG501.3460522E-939.6000021
TATACGG950.039.368422
AATCCGG452.348861E-839.111112
AATGCGG900.039.111112
CAACGCG404.1215753E-738.51
GCCGATT800.038.59
GCGATAT1200.038.59
GCTACGA1150.038.2608710
TATGGGC3350.038.0895544
TTCGAAT357.286284E-637.71428716
TGCGTTG357.286284E-637.7142871
TGGGCGA4400.037.56
GGGCGAT15800.037.177217
Produced by FastQC (version 0.11.5)