##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547194_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	710626
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.54201788282444	31.0	30.0	31.0	27.0	33.0
2	30.864671430541524	31.0	31.0	33.0	28.0	34.0
3	31.035636185560335	31.0	31.0	34.0	28.0	34.0
4	34.86327688545029	35.0	35.0	37.0	32.0	37.0
5	34.65243602119821	35.0	35.0	37.0	32.0	37.0
6	34.70225406894766	35.0	35.0	37.0	31.0	37.0
7	35.353111200547126	35.0	35.0	37.0	33.0	37.0
8	34.31613535108482	35.0	35.0	37.0	32.0	37.0
9	36.41538727825889	39.0	37.0	39.0	32.0	39.0
10	35.739870198951344	37.0	35.0	39.0	30.0	39.0
11	35.52297551736075	37.0	35.0	39.0	30.0	39.0
12	34.96791842685182	37.0	34.0	39.0	30.0	39.0
13	34.64684517594346	37.0	33.0	39.0	27.0	39.0
14	35.54429193415383	38.0	33.0	40.0	27.0	41.0
15	35.8498957257404	38.0	33.0	40.0	29.0	41.0
16	35.99365629740539	38.0	34.0	40.0	30.0	41.0
17	35.9593386675973	38.0	34.0	40.0	30.0	41.0
18	35.897069625935444	38.0	34.0	40.0	29.0	41.0
19	35.85504189264114	37.0	34.0	40.0	29.0	41.0
20	35.694179498076345	37.0	34.0	40.0	29.0	41.0
21	35.51319118636245	37.0	34.0	40.0	29.0	41.0
22	35.44535240759556	37.0	34.0	40.0	28.0	41.0
23	35.3573848972596	37.0	34.0	40.0	28.0	41.0
24	35.26762178698781	37.0	34.0	40.0	27.0	41.0
25	35.063301933787955	36.0	33.0	40.0	27.0	41.0
26	34.82533287552102	36.0	33.0	40.0	27.0	41.0
27	34.64004975894493	36.0	33.0	40.0	26.0	41.0
28	34.636351611114705	36.0	33.0	40.0	25.0	41.0
29	34.534131878090584	36.0	33.0	40.0	25.0	41.0
30	34.574978962210785	36.0	33.0	40.0	25.0	41.0
31	34.53315668157371	36.0	33.0	40.0	25.0	41.0
32	34.30410933458669	36.0	33.0	40.0	24.0	41.0
33	34.159870311528145	36.0	33.0	40.0	24.0	41.0
34	34.05051743111004	36.0	33.0	40.0	24.0	41.0
35	33.84620039233014	36.0	32.0	40.0	23.0	41.0
36	33.75867474592824	36.0	32.0	40.0	23.0	41.0
37	33.74133088291168	36.0	32.0	40.0	23.0	41.0
38	33.79989192627345	36.0	32.0	40.0	23.0	41.0
39	33.72688446524614	36.0	32.0	40.0	23.0	41.0
40	33.61382077210797	36.0	32.0	40.0	23.0	41.0
41	33.6625257167624	36.0	32.0	40.0	23.0	41.0
42	33.511702358202484	35.0	32.0	40.0	23.0	41.0
43	33.56900113421124	35.0	32.0	40.0	23.0	41.0
44	33.19547976009884	35.0	31.0	39.0	21.0	41.0
45	33.14222530557565	35.0	31.0	39.0	22.0	41.0
46	33.102464587560824	35.0	31.0	39.0	22.0	41.0
47	33.06589964341299	35.0	31.0	39.0	22.0	41.0
48	33.090259855395104	35.0	31.0	39.0	22.0	41.0
49	33.09987813561564	35.0	31.0	39.0	22.0	40.0
50	32.51329954153099	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	5.0
14	9.0
15	30.0
16	126.0
17	286.0
18	664.0
19	1161.0
20	2055.0
21	3134.0
22	4607.0
23	6332.0
24	8836.0
25	11744.0
26	14941.0
27	18082.0
28	20711.0
29	24191.0
30	28311.0
31	34002.0
32	40980.0
33	50038.0
34	69097.0
35	75980.0
36	62242.0
37	71199.0
38	83135.0
39	78726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.73208973496607	24.457590912800825	28.035563010641322	13.774756341591779
2	30.765972536890008	26.1469464950621	29.538322549414183	13.54875841863371
3	29.375649075603764	25.868037476816212	30.43415242335631	14.322161024223712
4	24.918452181597633	29.166115509424085	30.916684725861426	14.99874758311686
5	21.729714364518042	33.62232735644347	30.685480125973434	13.962478153065044
6	20.954482385952666	42.1587445435433	27.165344358354464	9.721428712149569
7	88.89246945650736	3.6598717187381267	6.083509469115963	1.3641493556385496
8	87.13120544421398	5.857792988154106	5.592956069718811	1.4180454979131074
9	83.93613518221962	5.512041495807921	7.620604931426659	2.9312183905458005
10	43.034169872760074	28.929000627615654	15.132573252315565	12.904256247308709
11	38.57528432677667	24.862867387345805	21.473742868963424	15.088105416914102
12	35.54851637851697	21.851719469875857	26.032540323602007	16.56722382800517
13	23.012104820257072	33.61247688657606	26.280490722264595	17.094927570902275
14	18.883913619822522	35.98573651963199	27.168018057318477	17.962331803227013
15	18.228857373639578	24.86174161936096	39.78829933044949	17.121101676549973
16	19.33520586074813	20.915361948479227	39.83670735379792	19.912724836974725
17	19.990121385932966	21.785158437771766	28.644603490443636	29.580116685851632
18	22.646652388176058	24.393281416666433	30.89754667011902	22.06251952503849
19	28.43324055128858	25.959506125585047	25.30233906443052	20.304914258695856
20	29.96231491670724	24.475884642554593	25.230993518390825	20.33080692234734
21	24.237503271763206	27.23823783537332	27.20165037586579	21.32260851699769
22	24.096219389664885	25.471344983155696	25.23634091631885	25.196094710860567
23	21.901956866199658	29.28446186883114	25.4260328217656	23.3875484432036
24	21.843135488991397	25.809356820606055	33.74151804183917	18.60598964856338
25	21.489503620751282	26.032821765598218	30.878408614376617	21.59926599927388
26	20.19740341614295	31.836437169481556	26.6792940308967	21.28686538347879
27	21.112934229819906	30.68266570601132	27.125238873894286	21.07916119027449
28	18.922893336297854	29.084919493516985	33.23182658669961	18.760360583485546
29	20.325881687413634	25.219313675548037	32.47207391792589	21.982730719112446
30	20.91367329650196	28.995702380717848	29.053257268943156	21.037367053837038
31	26.17621646266813	27.416249897977274	25.442497178543988	20.96503646081061
32	27.268070686971768	27.100190536231434	26.134422326230677	19.49731645056612
33	26.947367532288435	27.265396988007755	24.705259869467202	21.081975610236608
34	21.864384359705387	27.688967192306503	27.059662888776938	23.386985559211173
35	22.124999648197505	26.600912434951717	28.70412847264243	22.569959444208347
36	27.42033080692235	27.68826358731597	24.60422219282717	20.28718341293451
37	22.275711837168917	31.905953342545867	26.870956030316933	18.94737878996828
38	22.617241699571927	30.90345695203947	24.911979015684764	21.567322332703842
39	22.22209713689057	31.078373152685096	25.427017868752337	21.27251184167199
40	24.575655830211673	27.218114732644178	25.395637086174727	22.81059235096943
41	20.091159062573	26.01227649987476	27.367138269638318	26.529426167913922
42	22.064911782006288	27.570620832899444	24.705541311463413	25.658926073630855
43	21.885070346426954	27.755528224410593	26.277676302302478	24.08172512685998
44	21.47515007894448	29.420116911005227	27.37501864553225	21.729714364518042
45	20.037825804290865	32.262258909749995	24.522885455921962	23.17702983003718
46	22.024665576548003	31.815188298767566	25.657800305646006	20.502345819038425
47	22.39419891757408	28.611956218883066	26.511413880156372	22.48243098338648
48	23.55275489497992	25.04369386991188	28.944620658405405	22.458930576702794
49	21.305018392234455	26.327069372637645	29.307962275514825	23.059949959613075
50	20.65573170697385	30.425287000475638	27.205590563812756	21.71339072873776
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	522.0
1	726.5
2	931.0
3	3147.5
4	5364.0
5	4056.5
6	2749.0
7	2832.5
8	2916.0
9	3103.0
10	3290.0
11	3290.5
12	3291.0
13	3239.0
14	3187.0
15	3245.0
16	3303.0
17	3196.5
18	3090.0
19	3079.0
20	3068.0
21	3122.5
22	3177.0
23	3409.5
24	3642.0
25	4664.0
26	5686.0
27	6727.0
28	7768.0
29	9410.5
30	11053.0
31	12580.0
32	14107.0
33	15481.5
34	16856.0
35	19035.0
36	21214.0
37	22973.0
38	24732.0
39	28501.0
40	32270.0
41	40578.5
42	48887.0
43	53252.0
44	57617.0
45	58481.5
46	59346.0
47	57822.0
48	56298.0
49	55867.5
50	55437.0
51	51497.5
52	47558.0
53	42900.5
54	38243.0
55	36419.5
56	34596.0
57	33221.5
58	31847.0
59	30235.5
60	28624.0
61	26235.0
62	23846.0
63	20613.5
64	17381.0
65	14464.5
66	11548.0
67	10008.5
68	8469.0
69	7926.0
70	7383.0
71	6085.5
72	4788.0
73	3794.0
74	2800.0
75	2308.5
76	1817.0
77	1446.5
78	1076.0
79	807.5
80	539.0
81	371.5
82	204.0
83	132.5
84	61.0
85	41.5
86	22.0
87	14.0
88	6.0
89	8.0
90	10.0
91	8.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	710626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.007223976990367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.88925429387095	23.072330750199534
2	6.806534398238797	4.084904043900818
3	2.8341489564772138	2.551348275634958
4	1.6918740577507154	2.03073775167141
5	1.1806733293090208	1.7714364518117345
6	0.935532238767306	1.6843635255831466
7	0.7350080286887719	1.5438885379225094
8	0.6288674338082582	1.5096452750495652
9	0.5890755841699699	1.5908870694208253
>10	7.182917593232256	49.039423247953
>50	0.49346516193052314	9.081712115728179
>100	0.031249684166735674	1.340654353656994
>500	4.6641319651844295E-4	0.11084646080972327
>1k	9.328263930368859E-4	0.5878221406576234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2954	0.4156898284048149	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1246	0.17533836363994562	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	792	0.11145103049986912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012524168831424688	0.0
2	0.0	0.0	0.0	0.05980642419500553	0.0
3	0.0	0.0	0.0	0.09484595272337347	0.0
4	0.0	0.0	0.0	0.15338588793542596	0.0
5	0.0	0.0	0.0	0.251468423615235	0.0
6	0.0	0.0	0.0	0.44791493697106494	0.0
7	0.0	0.0	0.0	0.5495154976035214	0.0
8	0.0	0.0	0.0	0.8370084967338657	0.0
9	0.0	0.0	0.0	0.9836397767602086	0.0
10	0.0	0.0	0.0	1.203727417797829	0.0
11	0.0	0.0	0.0	1.3580983527199961	0.0
12	0.0	0.0	0.0	1.5027595387728565	0.0
13	0.0	0.0	0.0	1.5731200378258043	0.0
14	0.0	0.0	0.0	1.6061894723806898	0.0
15	0.0	0.0	0.0	1.6388367439412574	0.0
16	0.0	0.0	0.0	1.7180626658748765	0.0
17	0.0	0.0	0.0	1.8057318476948494	0.0
18	0.0	0.0	0.0	1.9449049148215798	0.0
19	0.0	0.0	0.0	1.9975345681131846	0.0
20	0.0	0.0	0.0	2.06043685426652	0.0
21	0.0	0.0	0.0	2.143602964147104	0.0
22	0.0	0.0	0.0	2.2376045908818423	0.0
23	0.0	0.0	0.0	2.349899947370347	0.0
24	0.0	0.0	0.0	2.4326438942566133	0.0
25	2.8144199621179074E-4	0.0	0.0	2.505115208281149	0.0
26	2.8144199621179074E-4	0.0	0.0	2.574209218351144	0.0
27	2.8144199621179074E-4	0.0	0.0	2.6462583693813624	0.0
28	2.8144199621179074E-4	0.0	0.0	2.724921407322558	0.0
29	2.8144199621179074E-4	0.0	0.0	2.807946796205036	0.0
30	2.8144199621179074E-4	0.0	0.0	2.9193978267049054	0.0
31	2.8144199621179074E-4	0.0	0.0	3.01522882641502	0.0
32	2.8144199621179074E-4	0.0	0.0	3.1110598261251345	0.0
33	2.8144199621179074E-4	0.0	0.0	3.211816060768956	0.0
34	2.8144199621179074E-4	0.0	0.0	3.3093357124563414	0.0
35	2.8144199621179074E-4	0.0	0.0	3.4314815388122586	0.0
36	2.8144199621179074E-4	0.0	0.0	3.543917616298869	0.0
37	2.8144199621179074E-4	0.0	0.0	3.658183066760856	0.0
38	2.8144199621179074E-4	0.0	0.0	3.7730114012152667	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.854451E-4	44.0	1
CTATGCG	35	1.4450416E-7	44.0	1
GTATACG	20	7.854451E-4	44.0	1
CGGTCTA	30	2.526347E-6	44.0	31
CGTTTTT	2450	0.0	41.485714	1
ACGTAGG	140	0.0	40.857143	2
TAAGGGA	790	0.0	39.822784	4
TGCGTAG	50	1.3460522E-9	39.600002	1
TATACGG	95	0.0	39.36842	2
AATCCGG	45	2.348861E-8	39.11111	2
AATGCGG	90	0.0	39.11111	2
CAACGCG	40	4.1215753E-7	38.5	1
GCCGATT	80	0.0	38.5	9
GCGATAT	120	0.0	38.5	9
GCTACGA	115	0.0	38.26087	10
TATGGGC	335	0.0	38.089554	4
TTCGAAT	35	7.286284E-6	37.714287	16
TGCGTTG	35	7.286284E-6	37.714287	1
TGGGCGA	440	0.0	37.5	6
GGGCGAT	1580	0.0	37.17721	7
>>END_MODULE