FastQCFastQC Report
Thu 26 May 2016
SRR1547193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547193_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1644754
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52720.32053425618663944No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29090.17686535494061725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAA351.4465877E-744.017
TAACCGT207.8574725E-444.039
TACGAAC254.4436798E-544.030
ACTCGAC207.8574725E-444.018
CGTCCAT207.8574725E-444.028
AAGTCGA207.8574725E-444.013
CGACGGT1950.041.7435928
CGTTGAT1500.041.06666625
CGCGTGA650.040.61538340
CGTTTTT45100.040.4390261
GTATGCG1200.040.3333321
CGCATAT452.3519533E-839.1111124
AGGGCGC3100.039.0322576
CGTAAGG4200.038.2380942
GACCGAT9800.038.1632659
CTAAGCG357.292123E-637.7142871
TTTAGCG1050.037.7142831
CGGTCTA2100.037.71428331
AGTAACG651.0913936E-1137.2307661
CTCACGA2250.037.15555224
Produced by FastQC (version 0.11.5)