##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547193_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1644754
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.67372081174449	31.0	30.0	31.0	28.0	33.0
2	30.9945152892165	31.0	31.0	33.0	28.0	34.0
3	31.16681217981534	31.0	31.0	34.0	28.0	34.0
4	34.98466214400452	35.0	35.0	37.0	32.0	37.0
5	34.77757281636038	35.0	35.0	37.0	32.0	37.0
6	34.81622114918097	35.0	35.0	37.0	32.0	37.0
7	35.481332770736536	35.0	35.0	37.0	35.0	37.0
8	34.45641719065587	36.0	35.0	37.0	33.0	37.0
9	36.59285279135968	39.0	37.0	39.0	32.0	39.0
10	36.007985388696426	38.0	35.0	39.0	30.0	39.0
11	35.772907073033416	37.0	35.0	39.0	30.0	39.0
12	35.255846163012826	37.0	34.0	39.0	30.0	39.0
13	34.89978683742371	37.0	33.0	39.0	27.0	39.0
14	35.801135002559654	38.0	34.0	40.0	27.0	41.0
15	36.10008852387652	38.0	34.0	40.0	30.0	41.0
16	36.2465475080164	38.0	34.0	40.0	30.0	41.0
17	36.190978103716425	38.0	34.0	40.0	30.0	41.0
18	36.15011667398286	38.0	34.0	40.0	30.0	41.0
19	36.173120722004626	38.0	34.0	40.0	30.0	41.0
20	36.032576908157694	38.0	34.0	40.0	30.0	41.0
21	35.867816098942455	38.0	34.0	40.0	29.0	41.0
22	35.792214519618135	37.0	34.0	40.0	29.0	41.0
23	35.69852938494146	37.0	34.0	40.0	29.0	41.0
24	35.62167169072092	37.0	34.0	40.0	29.0	41.0
25	35.434780520369614	37.0	34.0	40.0	29.0	41.0
26	35.26867786915247	37.0	34.0	40.0	27.0	41.0
27	35.09416058571677	37.0	33.0	40.0	27.0	41.0
28	35.142217620385786	37.0	34.0	40.0	27.0	41.0
29	35.0642971532521	37.0	34.0	40.0	27.0	41.0
30	35.10121452813004	37.0	34.0	40.0	27.0	41.0
31	35.03992329552018	37.0	33.0	40.0	27.0	41.0
32	34.82207126415257	37.0	33.0	40.0	26.0	41.0
33	34.70527872253237	37.0	33.0	40.0	25.0	41.0
34	34.66057416489031	37.0	33.0	40.0	25.0	41.0
35	34.480434764104544	37.0	33.0	40.0	25.0	41.0
36	34.38036144006946	37.0	33.0	40.0	24.0	41.0
37	34.378366612879496	37.0	33.0	40.0	24.0	41.0
38	34.412853229115115	37.0	33.0	40.0	24.0	41.0
39	34.342088847329144	37.0	33.0	40.0	24.0	41.0
40	34.26124757866526	36.0	33.0	40.0	24.0	41.0
41	34.29744995300209	36.0	33.0	40.0	24.0	41.0
42	34.16733991830997	36.0	33.0	40.0	23.0	41.0
43	34.214418691184214	36.0	33.0	40.0	24.0	41.0
44	33.92170987272261	36.0	33.0	40.0	23.0	41.0
45	33.84773224445723	36.0	32.0	40.0	23.0	41.0
46	33.8147589244349	36.0	32.0	40.0	23.0	41.0
47	33.76949805259631	36.0	32.0	40.0	23.0	41.0
48	33.79116390657813	35.0	32.0	40.0	23.0	41.0
49	33.804269209863605	36.0	32.0	39.0	24.0	41.0
50	33.219297232291275	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	7.0
14	28.0
15	51.0
16	175.0
17	429.0
18	1034.0
19	1989.0
20	3256.0
21	5304.0
22	8122.0
23	11680.0
24	16227.0
25	21939.0
26	28496.0
27	35134.0
28	41438.0
29	49917.0
30	60886.0
31	74019.0
32	90716.0
33	112236.0
34	157726.0
35	180939.0
36	146726.0
37	171527.0
38	209071.0
39	215679.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.6692295625972	24.615778408199645	27.936335768145266	13.778656261057884
2	28.97071537749718	27.335394837161058	30.175637207752647	13.51825257758911
3	27.95840593790925	26.87131327845988	31.420078625739777	13.750202157891088
4	24.86560300202948	29.44580162139749	30.619107781467626	15.069487595105407
5	22.433932369217523	33.76389417505597	29.808713035505612	13.993460420220897
6	20.337631037833013	42.762443502189385	26.5581357455279	10.3417897144497
7	90.54934658921638	3.411209214265477	5.006766969406975	1.0326772271111668
8	88.86964251188931	5.462519015001636	4.299427148375988	1.3684113247330603
9	85.79763295909298	5.566181933590069	6.1083298779027135	2.5278552294142465
10	39.79920401470372	37.05234946989033	12.303116453889153	10.8453300615168
11	35.81623756500972	24.463901592578587	25.554520615240943	14.16534022717075
12	32.826185557232265	23.66031637557957	26.81811383343649	16.69538423375167
13	22.46475764764822	34.58255763475876	27.404280518545633	15.548404199047393
14	18.534504248051682	37.05611903056628	27.67818166120891	16.731195060173132
15	16.990990749984498	25.64164610634782	40.82276133695373	16.544601806713953
16	18.735081355631298	23.18906049172095	39.396408216669485	18.67944993597827
17	19.157089753239696	22.093455921067832	29.881733073760575	28.8677212519319
18	20.945867892706143	24.63888216718123	33.307898931998345	21.107351008114282
19	26.739317855436134	25.56771407760674	27.024710078224462	20.66825798873266
20	27.784702149987172	25.655751559199736	26.35530906141587	20.204237229397222
21	23.342396492119793	28.430695410985475	28.84625907582532	19.380649021069413
22	23.32312309317989	25.61531998098196	26.765218385241806	24.296338540596345
23	19.690725786348597	30.64810907892609	26.877150017571015	22.784015117154297
24	19.668838014681832	27.185402801877974	35.14981571712244	17.995943466317758
25	19.03707180526693	27.653314720620838	33.14629421785872	20.163319256253516
26	18.430415733903065	32.30556058839194	28.221484793470637	21.04253888423436
27	19.00715851732235	31.551466055106115	29.21707440747978	20.224301020091758
28	17.20348453324935	29.024279618715017	36.112999269191626	17.65923657884401
29	17.88054627014131	25.59738416808836	34.87457698841286	21.647492573357475
30	20.35526285389791	28.725693933560887	31.219744715623126	19.69929849691808
31	25.56880846619008	27.115361932544317	27.25483567755421	20.060993923711386
32	26.123237882382412	26.708796573834142	28.29304564694781	18.87491989683564
33	25.126371481692704	26.675964916333996	27.319587002068396	20.87807659990491
34	20.13243317845708	27.59452173394927	29.455286322453084	22.81775876514056
35	20.2064260065639	26.695967907662787	30.777003734297043	22.320602351476268
36	26.246721394202417	26.693900729227593	27.398200581971526	19.66117729459846
37	21.224693783994443	30.535812650402434	28.93058779610811	19.308905769495013
38	20.588246023417483	31.92106539944575	26.18020688808174	21.31048168905502
39	20.762861801825682	31.140401543331098	27.042159496192138	21.05457715865108
40	23.274969995513008	27.06392566912742	27.09402135516922	22.56708298019035
41	18.92161380972474	26.058304159771005	28.824857699084482	26.195224331419777
42	20.993838592275804	27.993791168770528	26.66866899244507	24.343701246508598
43	21.791039875872016	27.180538852618692	27.759713610667614	23.26870766084168
44	20.72516619506625	29.21214965885476	29.24285333855397	20.819830807525015
45	18.85832166998834	32.601835897647916	26.513569810439737	22.026272621924008
46	21.55629352474595	30.613392641087966	27.70092062399605	20.129393210170033
47	21.550517585000552	27.6498491567736	28.562569235277735	22.237064022948115
48	22.186904546211775	25.90478576127494	30.23637577412792	21.671933918385363
49	20.963317310673816	25.67338337526463	31.416309065063835	21.94699024899772
50	20.32303919005517	30.78770442266746	28.14402640151658	20.745229985760787
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1222.0
1	1485.0
2	1748.0
3	5724.0
4	9700.0
5	7409.0
6	5118.0
7	5319.0
8	5520.0
9	5990.0
10	6460.0
11	6761.0
12	7062.0
13	6988.5
14	6915.0
15	6846.5
16	6778.0
17	6641.0
18	6504.0
19	6472.0
20	6440.0
21	7246.0
22	8052.0
23	8908.5
24	9765.0
25	12771.0
26	15777.0
27	18684.5
28	21592.0
29	25669.0
30	29746.0
31	34273.0
32	38800.0
33	45308.5
34	51817.0
35	56810.5
36	61804.0
37	65328.0
38	68852.0
39	80964.0
40	93076.0
41	111720.5
42	130365.0
43	143268.0
44	156171.0
45	159574.5
46	162978.0
47	149937.0
48	136896.0
49	129648.0
50	122400.0
51	114437.0
52	106474.0
53	97748.0
54	89022.0
55	78119.0
56	67216.0
57	61139.5
58	55063.0
59	51126.0
60	47189.0
61	41188.5
62	35188.0
63	30852.0
64	26516.0
65	21485.0
66	16454.0
67	13252.5
68	10051.0
69	8855.5
70	7660.0
71	6765.5
72	5871.0
73	4460.5
74	3050.0
75	2377.5
76	1705.0
77	1345.0
78	985.0
79	771.0
80	557.0
81	328.5
82	100.0
83	80.5
84	61.0
85	38.0
86	15.0
87	11.0
88	7.0
89	7.0
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1644754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.797575633331142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.21501947456395	18.059103977671707
2	7.049746829018234	3.2143427306075916
3	2.746543542736041	1.8784360243728644
4	1.4723032962916802	1.3425978300964931
5	0.9538026028490179	1.0872193488859296
6	0.6689755363751854	0.9150612232416934
7	0.5009040330875992	0.7993578305548605
8	0.3887764089659234	0.7090527670284414
9	0.3417978954055016	0.7012947034638295
>10	4.323671670425021	24.15763560382043
>50	1.7370999362457957	28.441040390525114
>100	0.6003037586438356	18.114968835699706
>500	5.275076961248751E-4	0.08796943362586261
>1k	2.6375384806243753E-4	0.17491666604076167
>5k	2.6375384806243753E-4	0.3170026343646942
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5272	0.32053425618663944	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2909	0.17686535494061725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017935812893599894	0.0
2	0.0	0.0	0.0	0.07393202874107617	0.0
3	0.0	0.0	0.0	0.11928835558387454	0.0
4	0.0	0.0	0.0	0.21492575789449364	0.0
5	0.0	0.0	0.0	0.3547034997330908	0.0
6	0.0	0.0	0.0	0.619302339437995	0.0
7	0.0	0.0	0.0	0.7505681700728498	0.0
8	0.0	0.0	0.0	1.180419685861837	0.0
9	0.0	0.0	0.0	1.3910286887887187	0.0
10	0.0	0.0	0.0	1.718798069498539	0.0
11	0.0	0.0	0.0	1.9260023079439235	0.0
12	0.0	0.0	0.0	2.1378880975513663	0.0
13	0.0	0.0	0.0	2.224770391195279	0.0
14	0.0	0.0	0.0	2.262162001126004	0.0
15	0.0	0.0	0.0	2.305390350167867	0.0
16	0.0	0.0	0.0	2.389354274256211	0.0
17	0.0	0.0	0.0	2.4936251865020544	0.0
18	0.0	0.0	0.0	2.661005840387073	0.0
19	0.0	0.0	0.0	2.7335394837161058	0.0
20	0.0	0.0	0.0	2.814585038248881	0.0
21	0.0	0.0	0.0	2.908520058318752	0.0
22	0.0	0.0	0.0	3.0144933528053435	0.0
23	0.0	0.0	0.0	3.1416856259355503	0.0
24	0.0	0.0	0.0	3.2316686872322546	0.0
25	0.0	0.0	0.0	3.30912707918631	0.0
26	0.0	0.0	0.0	3.3829983085616453	0.0
27	0.0	0.0	0.0	3.463739866265715	0.0
28	0.0	0.0	0.0	3.553966125025384	0.0
29	0.0	0.0	0.0	3.654528275961025	0.0
30	0.0	0.0	0.0	3.7789845776328863	0.0
31	0.0	0.0	0.0	3.8894570251843135	0.0
32	0.0	0.0	0.0	3.990809567874588	0.0
33	0.0	0.0	0.0	4.089790935300963	0.0
34	0.0	0.0	0.0	4.202391360653325	0.0
35	0.0	0.0	0.0	4.324476487061287	0.0
36	0.0	0.0	0.0	4.434888135246973	0.0
37	0.0	0.0	0.0	4.556000471803078	0.0
38	0.0	0.0	0.0	4.678085598211039	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAAA	35	1.4465877E-7	44.0	17
TAACCGT	20	7.8574725E-4	44.0	39
TACGAAC	25	4.4436798E-5	44.0	30
ACTCGAC	20	7.8574725E-4	44.0	18
CGTCCAT	20	7.8574725E-4	44.0	28
AAGTCGA	20	7.8574725E-4	44.0	13
CGACGGT	195	0.0	41.74359	28
CGTTGAT	150	0.0	41.066666	25
CGCGTGA	65	0.0	40.615383	40
CGTTTTT	4510	0.0	40.439026	1
GTATGCG	120	0.0	40.333332	1
CGCATAT	45	2.3519533E-8	39.11111	24
AGGGCGC	310	0.0	39.032257	6
CGTAAGG	420	0.0	38.238094	2
GACCGAT	980	0.0	38.163265	9
CTAAGCG	35	7.292123E-6	37.714287	1
TTTAGCG	105	0.0	37.714283	1
CGGTCTA	210	0.0	37.714283	31
AGTAACG	65	1.0913936E-11	37.230766	1
CTCACGA	225	0.0	37.155552	24
>>END_MODULE