FastQCFastQC Report
Thu 26 May 2016
SRR1547192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547192_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2107029
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187160.8882649455702792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT157500.042.686981
AACGGGA9350.036.470594
GTTTTTT183950.036.441422
TAGGGAT36950.036.438435
GGGCGAT41300.036.32937
CGTTCGA850.036.2352914
AGGGCGA22200.036.1711736
CGTTAGG2950.035.796612
ACGGGAC7400.035.6756745
AGGGATT44000.035.66
TGGGCGA11650.035.3133056
TACGGGA8350.035.305394
ACGGGAT10300.035.242725
TACGTCC250.00235318835.226
TAAGGGA33200.035.186754
AGGGATC22900.034.9694336
GTAGGGA31400.034.891724
AAGGGAT35950.034.8817835
TAGCACG950.034.7368431
TATGGGA24600.034.699194
Produced by FastQC (version 0.11.5)