##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547192_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2107029
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.595707985034853	31.0	30.0	31.0	28.0	33.0
2	30.929202208417635	31.0	31.0	33.0	28.0	34.0
3	31.048966103456575	31.0	31.0	34.0	28.0	34.0
4	34.887830684817345	35.0	35.0	37.0	32.0	37.0
5	34.70177866559976	35.0	35.0	37.0	32.0	37.0
6	34.743105102018056	35.0	35.0	37.0	32.0	37.0
7	35.42871597875492	35.0	35.0	37.0	35.0	37.0
8	34.40438646074639	36.0	35.0	37.0	33.0	37.0
9	36.50954732943875	39.0	37.0	39.0	32.0	39.0
10	35.90349919246484	38.0	35.0	39.0	30.0	39.0
11	35.65875837494406	37.0	35.0	39.0	30.0	39.0
12	35.477573872974695	37.0	35.0	39.0	30.0	39.0
13	35.41916746281138	37.0	35.0	39.0	30.0	39.0
14	36.34342147165511	38.0	35.0	40.0	30.0	41.0
15	36.46422094807428	38.0	35.0	40.0	30.0	41.0
16	36.49502166320445	38.0	35.0	40.0	30.0	41.0
17	36.40652786459038	38.0	34.0	40.0	30.0	41.0
18	36.27235125857309	38.0	35.0	40.0	30.0	41.0
19	36.22122666560356	38.0	34.0	40.0	30.0	41.0
20	36.044021700697996	38.0	34.0	40.0	30.0	41.0
21	35.88398071407655	38.0	34.0	40.0	29.0	41.0
22	35.837039262392686	38.0	34.0	40.0	29.0	41.0
23	35.70249199227918	37.0	34.0	40.0	29.0	41.0
24	35.6094016741108	37.0	34.0	40.0	29.0	41.0
25	35.4118918154425	37.0	34.0	40.0	27.0	41.0
26	35.24545889021935	37.0	34.0	40.0	27.0	41.0
27	35.060201354608786	37.0	33.0	40.0	27.0	41.0
28	35.04524427523304	37.0	33.0	40.0	27.0	41.0
29	34.886578210361606	37.0	33.0	40.0	26.0	41.0
30	34.86036025133019	37.0	33.0	40.0	26.0	41.0
31	34.77974769212953	37.0	33.0	40.0	25.0	41.0
32	34.45601223333898	37.0	33.0	40.0	24.0	41.0
33	34.18339804530455	37.0	33.0	40.0	23.0	41.0
34	33.98321807625808	37.0	33.0	40.0	23.0	41.0
35	33.720720502660384	37.0	32.0	40.0	21.0	41.0
36	33.646479948780964	37.0	32.0	40.0	20.0	41.0
37	33.63290111336863	37.0	32.0	40.0	20.0	41.0
38	33.635757267697784	37.0	32.0	40.0	20.0	41.0
39	33.51246518201695	37.0	32.0	40.0	19.0	41.0
40	33.45136018536052	37.0	32.0	40.0	18.0	41.0
41	33.466569752955465	37.0	32.0	40.0	18.0	41.0
42	33.31750251183064	37.0	32.0	40.0	18.0	41.0
43	33.365478121088984	37.0	32.0	40.0	18.0	41.0
44	33.080240945900606	36.0	31.0	40.0	18.0	41.0
45	33.01081190624334	36.0	31.0	40.0	18.0	41.0
46	32.9466144035037	36.0	31.0	40.0	18.0	41.0
47	32.8881121237534	36.0	31.0	40.0	18.0	41.0
48	32.88186114192069	36.0	31.0	40.0	18.0	41.0
49	32.87495710785186	36.0	31.0	39.0	18.0	41.0
50	32.32149960916532	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	0.0
12	1.0
13	13.0
14	38.0
15	101.0
16	311.0
17	911.0
18	1999.0
19	3778.0
20	6216.0
21	9721.0
22	14045.0
23	19495.0
24	27067.0
25	37371.0
26	49955.0
27	57830.0
28	62726.0
29	68113.0
30	78523.0
31	92221.0
32	110429.0
33	133200.0
34	172474.0
35	194720.0
36	195722.0
37	228111.0
38	274191.0
39	267743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.84631061081741	23.32212798210181	26.85397305874765	17.977588348333125
2	34.305365517038446	24.640382263367044	28.00160795129066	13.052644268303853
3	27.59406728621201	25.067951129291526	34.37418279482627	12.963798789670195
4	23.8899891743303	28.17203749924657	33.89920119751555	14.038772128907576
5	21.427327293549354	31.906917275462277	32.73941649592862	13.926338935059746
6	19.952976442184706	40.7356044933411	29.613166216506748	9.698252847967446
7	86.07109821459505	2.909404664102867	9.810116519516344	1.2093806017857371
8	84.32290205782644	5.246439417777354	9.21506063751377	1.2155978868824302
9	80.97349395760571	5.329589673421676	11.095813109359197	2.6011032596134176
10	34.99662320736924	36.33917710672231	17.57284783455757	11.091351851350883
11	26.64837550883258	25.981844578313822	31.592303665492977	15.77747624736062
12	25.690913603941855	23.206894636950892	33.542964999532515	17.559226759574738
13	22.440460003160847	26.92492604515647	33.41529708418821	17.219316867494467
14	19.87053808941405	29.094236481794983	33.26033955868667	17.774885870104303
15	18.998741830321272	25.340515009522885	38.15780418779238	17.502938972363456
16	21.217648167158593	24.331985938494437	36.7562572703081	17.694108624038872
17	21.6299823115866	23.564080038765482	33.262522727499245	21.543414922148674
18	22.037000914557893	24.18248633502434	34.54147997013804	19.23903278027972
19	24.71128778958429	25.084657116726916	31.48884044785335	18.71521464583544
20	25.742075690462734	24.965342195100305	31.00811616736172	18.284465947075244
21	23.963410090701174	25.460351993256857	32.47439878615814	18.10183912988383
22	23.206562415609845	23.982346707140717	32.07554333613823	20.735547541111206
23	20.581206998100168	26.926539691670122	32.26543156264105	20.226821747588666
24	20.178602192945615	25.78958334223212	35.107395294511846	18.924419170310422
25	20.546893279589412	26.886768051127913	33.305284360110846	19.261054309171826
26	20.17974123754348	28.53297225619581	31.450255312100595	19.83703119416012
27	20.159760496889223	27.153067186071006	32.676294441130146	20.01087787590963
28	19.086258423590753	27.286904926320428	34.7443248289416	18.882511821147215
29	20.16559810045329	25.46903720831559	34.71281126173394	19.652553429497175
30	21.05001877050577	26.52668757762708	33.19536655641664	19.227927095450514
31	23.15625461253737	26.251893068391556	30.962032321339667	19.629819997731403
32	23.5013851256912	26.68278414772649	31.142950571634277	18.672880154948032
33	23.13456530498631	26.87077396656619	30.227538396481492	19.767122331966007
34	21.26558296065218	27.72885423029299	30.766733632996985	20.238829176057852
35	21.371988710169628	27.761649222673253	30.661182166927937	20.205179900229187
36	23.54319755447125	27.918979757753693	29.12418386268058	19.41363882509448
37	22.018254138884657	29.040416624545745	29.41017897712846	19.531150259441137
38	21.85874043499164	29.8395513303329	28.70586973411377	19.59583850056169
39	21.904776820822114	28.875397538429702	28.889255914370427	20.330569726377757
40	22.847573526515298	27.67413262940377	29.25536383220165	20.222930011879285
41	20.74840925302879	27.189943754926958	29.632340133904183	22.429306858140063
42	21.720014295009705	27.961978691323186	28.292206704321583	22.025800309345527
43	21.320684243074016	27.664877892046096	29.220860272924575	21.79357759195531
44	20.91769975638684	28.582568156394622	29.779371807412236	20.720360279806304
45	20.878972240059344	29.42531877824178	28.34450783544033	21.351201146258546
46	21.403217516227826	29.197082716944095	28.827747506085583	20.571952260742496
47	21.434588702860758	28.01774441642711	29.52209010886893	21.025576771843195
48	21.851953627596014	27.33730764977606	30.067122948948494	20.743615773679434
49	21.646118776723053	26.80504160123093	30.364271208417158	21.18456841362886
50	20.61177136147628	28.849958875743997	29.74894982461086	20.789319938168862
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1017.0
1	1590.5
2	2164.0
3	17968.0
4	33772.0
5	25370.5
6	16969.0
7	17126.0
8	17283.0
9	17656.0
10	18029.0
11	17784.5
12	17540.0
13	17002.0
14	16464.0
15	15905.5
16	15347.0
17	14597.0
18	13847.0
19	13319.0
20	12791.0
21	14030.5
22	15270.0
23	16234.0
24	17198.0
25	19979.5
26	22761.0
27	27114.0
28	31467.0
29	35915.0
30	40363.0
31	47108.5
32	53854.0
33	62352.5
34	70851.0
35	76022.5
36	81194.0
37	85773.0
38	90352.0
39	100664.0
40	110976.0
41	122419.0
42	133862.0
43	142820.5
44	151779.0
45	152873.5
46	153968.0
47	151481.5
48	148995.0
49	148904.0
50	148813.0
51	143957.5
52	139102.0
53	128111.5
54	117121.0
55	106694.5
56	96268.0
57	88776.5
58	81285.0
59	74016.5
60	66748.0
61	59756.5
62	52765.0
63	46282.5
64	39800.0
65	33549.0
66	27298.0
67	22188.5
68	17079.0
69	14670.5
70	12262.0
71	10254.5
72	8247.0
73	6847.5
74	5448.0
75	4414.5
76	3381.0
77	2521.5
78	1662.0
79	1313.5
80	965.0
81	688.0
82	411.0
83	265.5
84	120.0
85	107.0
86	94.0
87	61.5
88	29.0
89	18.5
90	8.0
91	7.5
92	7.0
93	5.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2107029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.83531472221957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67117340602738	20.843303831948358
2	5.706011835303119	3.0624524681813785
3	2.368250454174948	1.9065823883647255
4	1.4713537851855152	1.579369675727294
5	1.1336923105740222	1.5211494976207092
6	0.8650321760587696	1.3928046413630122
7	0.7641194442939618	1.4353770041097196
8	0.6413513702494653	1.3768692694536941
9	0.5671297394060401	1.3697194540762312
>10	8.01475187672959	50.2108228658477
>50	0.7582918310951788	12.7129620344211
>100	0.03811914839274726	1.5519011091097217
>500	3.613112546970641E-4	0.07010136591079968
>1k	1.8065562734853206E-4	0.05924195653042684
>5k	0.0	0.0
>10k+	1.8065562734853206E-4	0.9073424373350809
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18716	0.8882649455702792	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012624410959697279	0.0
2	0.0	0.0	0.0	0.05054510403036693	0.0
3	0.0	0.0	0.0	0.09007944361468209	0.0
4	0.0	0.0	0.0	0.13151219086210963	0.0
5	0.0	2.8476114946685596E-4	0.0	0.21613371244534366	0.0
6	0.0	2.8476114946685596E-4	0.0	0.41470715400689784	0.0
7	0.0	2.8476114946685596E-4	0.0	0.5192619560528118	0.0
8	0.0	2.8476114946685596E-4	0.0	0.7849915686969662	0.0
9	0.0	2.8476114946685596E-4	0.0	0.9440781308657831	0.0
10	0.0	2.8476114946685596E-4	0.0	1.1520012301681657	0.0
11	0.0	2.8476114946685596E-4	0.0	1.2746858253968028	0.0
12	0.0	2.8476114946685596E-4	0.0	1.3921023393603031	0.0
13	4.7460191577809324E-5	2.8476114946685596E-4	0.0	1.4656181761143297	0.0
14	4.7460191577809324E-5	2.8476114946685596E-4	0.0	1.5079052068101577	0.0
15	4.7460191577809324E-5	2.8476114946685596E-4	0.0	1.5427409874282698	0.0
16	4.7460191577809324E-5	2.8476114946685596E-4	0.0	1.6017814657510647	0.0
17	4.7460191577809324E-5	2.8476114946685596E-4	0.0	1.6658052641895293	0.0
18	4.7460191577809324E-5	2.8476114946685596E-4	0.0	1.7631461171156164	0.0
19	4.7460191577809324E-5	2.8476114946685596E-4	0.0	1.8147353453606951	0.0
20	4.7460191577809324E-5	2.8476114946685596E-4	0.0	1.8729215402350894	0.0
21	4.7460191577809324E-5	2.8476114946685596E-4	0.0	1.9378945425051102	0.0
22	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.0124070432822707	0.0
23	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.0883908099983435	0.0
24	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.151750165754719	0.0
25	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.210695723694358	0.0
26	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.2674106526298403	0.0
27	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.331719212217772	0.0
28	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.398448241576172	0.0
29	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.471774237563887	0.0
30	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.5727695252414655	0.0
31	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.6583877108478338	0.0
32	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.7468535079488703	0.0
33	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.8321394722141937	0.0
34	4.7460191577809324E-5	2.8476114946685596E-4	0.0	2.921459552763631	0.0
35	4.7460191577809324E-5	2.8476114946685596E-4	0.0	3.019702149329696	0.0
36	4.7460191577809324E-5	2.8476114946685596E-4	0.0	3.1131512665464025	0.0
37	4.7460191577809324E-5	2.8476114946685596E-4	0.0	3.2109192611966897	0.0
38	4.7460191577809324E-5	2.8476114946685596E-4	0.0	3.313907876920536	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15750	0.0	42.68698	1
AACGGGA	935	0.0	36.47059	4
GTTTTTT	18395	0.0	36.44142	2
TAGGGAT	3695	0.0	36.43843	5
GGGCGAT	4130	0.0	36.3293	7
CGTTCGA	85	0.0	36.23529	14
AGGGCGA	2220	0.0	36.171173	6
CGTTAGG	295	0.0	35.79661	2
ACGGGAC	740	0.0	35.675674	5
AGGGATT	4400	0.0	35.6	6
TGGGCGA	1165	0.0	35.313305	6
TACGGGA	835	0.0	35.30539	4
ACGGGAT	1030	0.0	35.24272	5
TACGTCC	25	0.002353188	35.2	26
TAAGGGA	3320	0.0	35.18675	4
AGGGATC	2290	0.0	34.969433	6
GTAGGGA	3140	0.0	34.89172	4
AAGGGAT	3595	0.0	34.881783	5
TAGCACG	95	0.0	34.736843	1
TATGGGA	2460	0.0	34.69919	4
>>END_MODULE