FastQCFastQC Report
Thu 26 May 2016
SRR1547191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547191_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1700458
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185851.0929408429964163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT207.857549E-444.044
CGTTTTT137150.042.6846541
GCCGATA1500.039.69
GCGATAA2300.039.2173929
GTTAGCG1250.038.7199971
GCGATAC1050.037.7142839
GGGCGAT38200.037.434557
CGGTCTA1900.037.0526331
TGGGCGA10700.037.0093466
TCGATAG1250.036.9599951
CGACGGT1850.036.86486428
AGGGATC21850.036.8512576
GTTTTTT157450.036.845982
TAGGGAT30800.036.7857135
ATAGGGA27550.036.733214
AGGGCGA19350.036.723516
TACGGGA6550.036.6106874
TATAGCG1150.036.3478281
CGGGATT6800.036.235296
GACCGAT9600.036.2083329
Produced by FastQC (version 0.11.5)