##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547191_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1700458
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.64351839327993	31.0	30.0	31.0	28.0	34.0
2	30.9819777965701	31.0	31.0	33.0	28.0	34.0
3	31.09604941727464	31.0	31.0	34.0	28.0	34.0
4	34.935977248482466	35.0	35.0	37.0	32.0	37.0
5	34.75783053741992	35.0	35.0	37.0	32.0	37.0
6	34.79618902672104	35.0	35.0	37.0	32.0	37.0
7	35.45689455429067	35.0	35.0	37.0	35.0	37.0
8	34.42938020227491	36.0	35.0	37.0	33.0	37.0
9	36.53198079576208	39.0	37.0	39.0	32.0	39.0
10	35.957951328406814	38.0	35.0	39.0	30.0	39.0
11	35.74706696666428	37.0	35.0	39.0	30.0	39.0
12	35.52045448931994	37.0	35.0	39.0	30.0	39.0
13	35.45268392397813	37.0	35.0	39.0	30.0	39.0
14	36.374317389785574	38.0	35.0	40.0	30.0	41.0
15	36.49781176600657	38.0	35.0	40.0	30.0	41.0
16	36.531038108556636	38.0	35.0	40.0	30.0	41.0
17	36.434775219382075	38.0	35.0	40.0	30.0	41.0
18	36.31002118252847	38.0	35.0	40.0	30.0	41.0
19	36.226293151609745	38.0	35.0	40.0	30.0	41.0
20	36.03440073203807	38.0	34.0	40.0	30.0	41.0
21	35.88199355702993	38.0	34.0	40.0	29.0	41.0
22	35.82925894082653	38.0	34.0	40.0	29.0	41.0
23	35.70068240438752	37.0	34.0	40.0	29.0	41.0
24	35.587035963252255	37.0	34.0	40.0	29.0	41.0
25	35.40778484384795	37.0	34.0	40.0	27.0	41.0
26	35.242005977213196	37.0	34.0	40.0	27.0	41.0
27	35.04705379374263	37.0	33.0	40.0	27.0	41.0
28	35.03551043307156	37.0	33.0	40.0	27.0	41.0
29	34.858552813418505	37.0	33.0	40.0	26.0	41.0
30	34.8167082044955	37.0	33.0	40.0	25.0	41.0
31	34.71013691605438	37.0	33.0	40.0	25.0	41.0
32	34.38110438481868	37.0	33.0	40.0	24.0	41.0
33	34.088382659260034	37.0	33.0	40.0	23.0	41.0
34	33.844946479125035	37.0	33.0	40.0	22.0	41.0
35	33.57488159072438	37.0	32.0	40.0	20.0	41.0
36	33.496577980755774	37.0	32.0	40.0	19.0	41.0
37	33.490901274833014	37.0	32.0	40.0	19.0	41.0
38	33.47979015065353	37.0	32.0	40.0	18.0	41.0
39	33.373951605979094	37.0	32.0	40.0	18.0	41.0
40	33.302878400995496	36.0	32.0	40.0	18.0	41.0
41	33.29461827342986	36.0	32.0	40.0	18.0	41.0
42	33.13759293084569	36.0	31.0	40.0	18.0	41.0
43	33.18201155218183	36.0	31.0	40.0	18.0	41.0
44	32.80518425036079	36.0	31.0	40.0	17.0	41.0
45	32.76067859364947	36.0	31.0	40.0	17.0	41.0
46	32.72733992841928	35.0	31.0	40.0	18.0	41.0
47	32.67244589398856	35.0	31.0	39.0	18.0	41.0
48	32.65490179704527	35.0	31.0	39.0	18.0	41.0
49	32.66798474293396	35.0	31.0	39.0	18.0	41.0
50	32.13174979917175	35.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	6.0
14	38.0
15	103.0
16	247.0
17	718.0
18	1610.0
19	3091.0
20	5134.0
21	7804.0
22	11315.0
23	16128.0
24	22399.0
25	31003.0
26	41320.0
27	48442.0
28	51994.0
29	55804.0
30	63614.0
31	74780.0
32	88811.0
33	107255.0
34	140234.0
35	159210.0
36	157647.0
37	183533.0
38	216445.0
39	211768.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.793586198541806	23.26426174595315	26.812541091870546	19.1296109636345
2	34.747050500512216	24.583200525975943	27.64766903975282	13.022079933759024
3	27.006018378578005	25.40944851328289	34.538577253892775	13.045955854246326
4	24.212947335364944	27.708123340888164	33.7391455713696	14.3397837523773
5	21.416877100169486	31.524683350015113	33.0347471093082	14.023692440507205
6	20.17556446557339	40.04638750266105	29.926525677199905	9.851522354565653
7	85.24579848487878	2.9617314864583544	10.562977738938569	1.2294922897242977
8	83.37295011108772	5.220887549119119	10.055879063170039	1.3502832766231214
9	79.75992350296214	5.640127542109243	12.065161268317123	2.534787686611489
10	33.60371147067437	36.56926545671813	18.691199665031423	11.135823407576076
11	28.09013806868502	24.584611910438248	31.597134419080035	15.728115601796691
12	26.14995489450489	22.80303306520949	33.59247920266187	17.454532837623745
13	22.601146279414134	26.17788854532132	34.27752993605252	16.94343523921202
14	19.832833271977314	28.301316468857213	34.121454337596106	17.744395921569364
15	18.73118889146336	24.508514764845707	38.928982662318035	17.8313136813729
16	21.923622929822436	23.077958996929063	37.02379006126585	17.974628011982656
17	21.411584408435843	22.890774132616038	33.50732567343621	22.190315785511903
18	22.496997867633308	23.32836212361611	35.03797212280456	19.13666788594602
19	24.8647129185196	25.011438094913252	31.603897302961908	18.51995168360524
20	25.912548266408226	24.863183918685436	31.011880328711438	18.212387486194896
21	24.505103919061806	25.10582443082981	32.44584694241199	17.943224707696398
22	23.647805473584175	23.18722367738574	32.22919942744837	20.935771421581713
23	21.029452065267122	26.51638558553049	32.22543573554889	20.228726613653496
24	20.678076141839433	24.91023006742889	35.517901647673746	18.89379214305793
25	21.036097333777136	25.857445464692454	33.441696295939096	19.664760905591315
26	20.18726719507333	27.584803623494373	31.719454405813025	20.508474775619273
27	20.07194532296593	26.581368078482388	33.04750837715486	20.299178221396826
28	19.747679742751657	26.257043690582183	35.00886231826955	18.986414248396606
29	20.486598316453566	24.750037930957426	34.61890855287223	20.14445519971678
30	21.11354705614605	25.806812047107307	33.68792407692516	19.391716819821482
31	23.777005959570893	25.567582380746835	31.27957291506171	19.375838744620566
32	24.2599934841084	25.750121437871442	30.890560072639257	19.099325005380905
33	23.950606248434244	26.41229598143559	29.906942717785444	19.73015505234472
34	21.533257510623606	27.226017931639596	30.961188103440367	20.279536454296434
35	21.76584190847407	27.329695881932984	30.62592548595731	20.278536723635632
36	24.055107506330646	27.962466582532468	28.513965061177636	19.468460849959246
37	22.341451538350256	29.51187268371227	28.634226778903095	19.51244899903438
38	21.885750780083953	29.37902612119794	28.34483415644491	20.390388942273198
39	21.987958538229112	28.719145077385033	28.273324010354855	21.019572374030997
40	23.138060451948828	26.9899638803193	28.978898626134843	20.893077041597028
41	20.865731467639893	26.582367809143182	29.584617791206842	22.967282932010082
42	21.642110537278782	27.466541367090514	28.401465957994848	22.48988213763586
43	21.24780500312269	27.157506977531938	29.44489072943878	22.1497972899066
44	21.527376742030675	27.538110320866494	29.80614634410259	21.12836659300024
45	20.880374581436296	28.69726861821933	28.810943875120703	21.611412925223675
46	22.025066188050513	28.416109071791247	29.054172464124374	20.50465227603387
47	21.28485384525816	27.847968018028084	29.620902133425233	21.246276003288525
48	21.453220250073805	26.954855691819496	30.723663859971843	20.868260198134855
49	21.708269183949266	26.382539292355357	30.684262710399196	21.22492881329618
50	20.850265046240484	28.08008195439111	30.083306967887474	20.986346031480927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	897.0
1	1414.0
2	1931.0
3	17347.5
4	32764.0
5	24072.0
6	15380.0
7	15425.5
8	15471.0
9	15702.5
10	15934.0
11	15724.0
12	15514.0
13	15074.0
14	14634.0
15	13993.5
16	13353.0
17	12671.5
18	11990.0
19	11525.0
20	11060.0
21	11733.5
22	12407.0
23	13112.5
24	13818.0
25	16112.0
26	18406.0
27	20526.0
28	22646.0
29	26664.5
30	30683.0
31	35985.0
32	41287.0
33	47202.5
34	53118.0
35	57670.5
36	62223.0
37	67771.0
38	73319.0
39	77619.0
40	81919.0
41	90731.0
42	99543.0
43	107414.0
44	115285.0
45	117148.0
46	119011.0
47	119137.0
48	119263.0
49	117153.0
50	115043.0
51	108137.0
52	101231.0
53	94068.5
54	86906.0
55	83121.0
56	79336.0
57	74597.0
58	69858.0
59	64910.5
60	59963.0
61	55256.0
62	50549.0
63	45238.5
64	39928.0
65	33951.0
66	27974.0
67	23523.0
68	19072.0
69	16946.5
70	14821.0
71	12607.0
72	10393.0
73	8192.5
74	5992.0
75	4788.5
76	3585.0
77	2824.0
78	2063.0
79	1597.5
80	1132.0
81	773.5
82	415.0
83	314.0
84	213.0
85	152.0
86	91.0
87	58.0
88	25.0
89	15.5
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1700458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.45492866697011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.22674014576495	22.45253193509173
2	5.900153857834161	3.475772220133074
3	2.774820516557476	2.451964211365368
4	1.7543720936978928	2.0669961950077775
5	1.3162668144333671	1.9385272562917406
6	1.0648117470420546	1.8818372431725323
7	0.8980943474390021	1.851731345801741
8	0.7638462638480421	1.7999229771340566
9	0.6915375113317761	1.833226926013035
>10	8.276438485348658	52.145197284357835
>50	0.3050332481692561	5.536807510533212
>100	0.02707081085607395	1.2881992096615371
>500	4.070788386668421E-4	0.08207482296936551
>1k	2.0353941933342105E-4	0.08099567993543667
>5k	0.0	0.0
>10k+	2.0353941933342105E-4	1.1142151825315254
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18585	1.0929408429964163	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009703268178337837	0.0
2	0.0	0.0	0.0	0.048751571635406464	0.0
3	0.0	0.0	0.0	0.09991425839391505	0.0
4	0.0	0.0	0.0	0.15254713730065664	0.0
5	0.0	0.0	0.0	0.24993266519961094	0.0
6	0.0	0.0	0.0	0.48675121643698344	0.0
7	0.0	0.0	0.0	0.6242435861397341	0.0
8	0.0	0.0	0.0	0.954978011806231	0.0
9	0.0	0.0	0.0	1.1595111434684067	0.0
10	0.0	0.0	0.0	1.4128546544519183	0.0
11	0.0	0.0	0.0	1.5565806388631769	0.0
12	0.0	0.0	0.0	1.6958960468297364	0.0
13	0.0	0.0	0.0	1.7868127292764655	0.0
14	0.0	0.0	0.0	1.8395632235550659	0.0
15	0.0	0.0	0.0	1.8837277956879852	0.0
16	0.0	0.0	0.0	1.9437116353358919	0.0
17	0.0	0.0	0.0	2.014104435393288	0.0
18	0.0	0.0	0.0	2.118899731719337	0.0
19	0.0	0.0	0.0	2.171650225997937	0.0
20	0.0	0.0	0.0	2.2310459887865504	0.0
21	0.0	0.0	0.0	2.294558289590216	0.0
22	0.0	0.0	0.0	2.362481166838581	0.0
23	0.0	0.0	0.0	2.4409306198682943	0.0
24	0.0	0.0	0.0	2.506030728192052	0.0
25	0.0	0.0	0.0	2.564603183377655	0.0
26	0.0	0.0	0.0	2.62729217657831	0.0
27	0.0	0.0	0.0	2.693156784819149	0.0
28	0.0	0.0	0.0	2.760256354464503	0.0
29	0.0	0.0	0.0	2.8342952310495173	0.0
30	0.0	0.0	0.0	2.9399138349785763	0.0
31	0.0	0.0	0.0	3.024890941146444	0.0
32	0.0	0.0	0.0	3.1109853933469687	0.0
33	0.0	0.0	0.0	3.1976091147208576	0.0
34	0.0	0.0	0.0	3.292642335182639	0.0
35	0.0	0.0	0.0	3.393438708865494	0.0
36	0.0	0.0	0.0	3.486237237261961	0.0
37	0.0	0.0	0.0	3.5893271106960594	0.0
38	0.0	0.0	0.0	3.6980037142934434	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	20	7.857549E-4	44.0	44
CGTTTTT	13715	0.0	42.684654	1
GCCGATA	150	0.0	39.6	9
GCGATAA	230	0.0	39.217392	9
GTTAGCG	125	0.0	38.719997	1
GCGATAC	105	0.0	37.714283	9
GGGCGAT	3820	0.0	37.43455	7
CGGTCTA	190	0.0	37.05263	31
TGGGCGA	1070	0.0	37.009346	6
TCGATAG	125	0.0	36.959995	1
CGACGGT	185	0.0	36.864864	28
AGGGATC	2185	0.0	36.851257	6
GTTTTTT	15745	0.0	36.84598	2
TAGGGAT	3080	0.0	36.785713	5
ATAGGGA	2755	0.0	36.73321	4
AGGGCGA	1935	0.0	36.72351	6
TACGGGA	655	0.0	36.610687	4
TATAGCG	115	0.0	36.347828	1
CGGGATT	680	0.0	36.23529	6
GACCGAT	960	0.0	36.208332	9
>>END_MODULE