FastQCFastQC Report
Thu 26 May 2016
SRR1547190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547190_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3820818
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510471.3360228097752889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT465400.043.1160281
CGACGGT1450.042.48275828
GTTTTTT512450.038.431852
TAGGGCG16100.038.2608685
CGTTCGA700.037.71428714
AGGGCGA40250.037.7142876
GACCGAT25050.036.886239
TAGGGAC43250.036.624285
TAGGGAT74150.036.5232665
GGGCGAT76550.036.41287
CGCCGGT3150.036.31746328
CGGTCTA1650.036.031
AGGGATC54000.035.892596
ACGGGAT22150.035.855535
TACGGGA15800.035.784814
GCGATCG3450.035.7101489
CGTAAGG5000.035.642
TAAGGGA61500.035.629274
GGACCGA26150.035.502878
GTAGGGA58850.035.401874
Produced by FastQC (version 0.11.5)