##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547190_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3820818
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.691082380788618	31.0	30.0	31.0	28.0	34.0
2	31.031537749246365	31.0	31.0	33.0	28.0	34.0
3	31.096975830829944	31.0	31.0	34.0	28.0	34.0
4	34.95350995519807	35.0	35.0	37.0	32.0	37.0
5	34.79454504244902	35.0	35.0	37.0	32.0	37.0
6	34.83883503480145	35.0	35.0	37.0	32.0	37.0
7	35.52866244872171	36.0	35.0	37.0	35.0	37.0
8	34.50654938288084	37.0	35.0	37.0	33.0	37.0
9	36.630690862532575	39.0	37.0	39.0	32.0	39.0
10	36.07850805769864	38.0	35.0	39.0	31.0	39.0
11	35.818481801540926	37.0	35.0	39.0	30.0	39.0
12	35.75136894769654	37.0	35.0	39.0	30.0	39.0
13	35.748283482751596	37.0	35.0	39.0	30.0	39.0
14	36.72446999569202	38.0	35.0	40.0	31.0	41.0
15	36.80664245195662	38.0	35.0	40.0	31.0	41.0
16	36.79775351770223	38.0	35.0	40.0	31.0	41.0
17	36.7116269343371	38.0	35.0	40.0	31.0	41.0
18	36.50433022457495	38.0	35.0	40.0	30.0	41.0
19	36.37601267581968	38.0	35.0	40.0	30.0	41.0
20	36.14085962744103	38.0	34.0	40.0	30.0	41.0
21	36.00131621029842	38.0	34.0	40.0	30.0	41.0
22	35.9646060084516	38.0	34.0	40.0	30.0	41.0
23	35.82089070979042	37.0	34.0	40.0	30.0	41.0
24	35.6921342497863	37.0	34.0	40.0	29.0	41.0
25	35.51389336000825	37.0	34.0	40.0	29.0	41.0
26	35.3733561766093	37.0	34.0	40.0	28.0	41.0
27	35.188114953394795	37.0	34.0	40.0	27.0	41.0
28	35.189058468631586	37.0	34.0	40.0	27.0	41.0
29	35.048878538574726	37.0	33.0	40.0	27.0	41.0
30	34.96538646959892	37.0	33.0	40.0	26.0	41.0
31	34.78895618686889	37.0	33.0	40.0	25.0	41.0
32	34.32297900606624	37.0	33.0	40.0	23.0	41.0
33	33.89196685107744	37.0	33.0	40.0	21.0	41.0
34	33.540652027916536	37.0	32.0	40.0	18.0	41.0
35	33.169248312795844	37.0	31.0	40.0	16.0	41.0
36	33.05780751661032	37.0	31.0	40.0	15.0	41.0
37	33.027164340201494	37.0	31.0	40.0	15.0	41.0
38	33.02318718138367	37.0	31.0	40.0	15.0	41.0
39	32.91053015349069	37.0	31.0	40.0	15.0	41.0
40	32.86807406162764	37.0	31.0	40.0	15.0	41.0
41	32.8836395766561	37.0	31.0	40.0	15.0	41.0
42	32.73048886390296	37.0	31.0	40.0	13.0	41.0
43	32.77990864783405	37.0	31.0	40.0	13.0	41.0
44	32.40362953692115	36.0	30.0	40.0	10.0	41.0
45	32.378342019954886	36.0	30.0	40.0	10.0	41.0
46	32.34456731516654	36.0	30.0	40.0	10.0	41.0
47	32.313443875107374	36.0	30.0	40.0	10.0	41.0
48	32.30989751409253	36.0	30.0	40.0	10.0	41.0
49	32.30860250344298	36.0	30.0	40.0	10.0	41.0
50	31.794188050831	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	7.0
13	14.0
14	44.0
15	187.0
16	578.0
17	1563.0
18	3700.0
19	7506.0
20	12313.0
21	18867.0
22	27501.0
23	39373.0
24	55534.0
25	80289.0
26	109175.0
27	124938.0
28	122741.0
29	123935.0
30	134382.0
31	157346.0
32	185398.0
33	224982.0
34	289813.0
35	333166.0
36	345513.0
37	407646.0
38	501706.0
39	512599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.374285820470902	22.41082930409143	25.89073858006322	22.324146295374444
2	37.33472779912574	23.69958474860619	26.734877191219265	12.230810261048813
3	25.5592388854952	24.38611313074844	37.759767672786296	12.29488031097006
4	22.58937745791608	26.620032673631666	37.278326264166466	13.512263604285785
5	20.350537502702302	30.443297744095638	36.61226470352684	12.593900049675227
6	18.670112002194294	38.868771032799785	33.286196830102874	9.17492013490305
7	82.14913665084282	2.4759096088847987	14.303036679580131	1.0719170606922392
8	80.59072690717014	4.790440162289855	13.556285591200629	1.062547339339377
9	77.43375894899994	5.070982182349434	15.41243262568382	2.08282624296682
10	31.191802383678052	37.77719849519134	21.15418740175533	9.876811719375276
11	22.000236598550362	25.373100733926606	37.60432975347164	15.02233291405139
12	22.127905595084613	23.43681379222983	37.785887733987856	16.649392878697704
13	20.500976492468368	25.303063375434263	38.25667697336016	15.939283158737213
14	18.24352795657893	28.283158213764697	36.91432044132958	16.558993388326794
15	17.531847892257627	25.911074539535772	40.85318902915554	15.70388853905106
16	20.173716727674545	24.701464450806085	39.61790381012652	15.506915011392849
17	20.311017169621792	23.533625522074068	36.89073910351134	19.264618204792797
18	20.52811727750445	24.27969612789722	38.082185542467606	17.110001052130723
19	21.98537067193465	24.84868423463248	35.860645547628806	17.305299545804065
20	23.24075629878209	25.02011349402144	35.3052409196146	16.433889287581874
21	22.287321720113336	24.883598224254598	36.19279955234717	16.636280503284897
22	21.500683884969135	23.625543011993766	36.306230760010024	18.567542343027068
23	18.978422945034286	26.31070100695715	36.50843876887096	18.20243727913761
24	18.668463140615437	25.30308954784028	38.5778123951468	17.450634916397483
25	18.8980998309786	26.461872824091593	36.872182867647716	17.76784447728209
26	18.762788491888386	27.990446024908806	35.62258134252927	17.62418414067354
27	18.404409736344416	26.85757343061093	36.86265611185877	17.87536072118588
28	17.86065182900625	26.558187278221574	38.3436740509493	17.23748684182288
29	18.877763871506048	25.153409557848605	37.787772147220835	18.181054423424513
30	19.171208887730323	26.09087896884908	36.94093777824539	17.796974365175206
31	20.702373156742876	26.556014968522447	35.04294106654648	17.698670808188194
32	20.924262814926017	26.903401313540716	34.611410436194554	17.560925435338714
33	20.93200984710604	27.493327345086836	33.53737341061521	18.03728939719191
34	19.694369111535803	28.18652969076255	33.39750807287864	18.72159312482301
35	19.67814221980738	28.185090208431806	33.21772981597134	18.919037755789468
36	21.38445222986282	28.72874342614592	31.35572016254111	18.53108418145015
37	20.327688992252444	29.61316137015686	31.453264719753726	18.605884917836963
38	20.21161437158221	29.741589366465504	30.7186052829525	19.328190978999785
39	20.44742774976458	29.258027992958574	30.837506523472197	19.457037733804647
40	21.220691485435843	28.36633412007586	31.021838779025853	19.39113561546245
41	19.466014869067305	28.187707449033166	31.19947089864003	21.1468067832595
42	20.331667197966507	29.051737088759527	30.184557338245373	20.432038375028593
43	20.20656309722159	28.803727369374833	30.644485029122038	20.345224504281546
44	20.029820839411876	29.974497607580364	30.77511150753582	19.220570045471938
45	20.023827358434765	29.745750779021662	30.31390136876449	19.916520493779082
46	20.228155332182794	29.80885244992041	30.400689067105525	19.562303150791273
47	20.15505580218686	28.63439190246696	31.231898509690858	19.97865378565532
48	20.635659693814258	28.07885641242268	31.324182413294743	19.961301480468318
49	20.23689691579133	27.961996619572037	31.696772785304088	20.10433367933254
50	19.381451825237423	29.099082971238094	31.420575384642763	20.098889818881716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1693.0
1	2686.0
2	3679.0
3	49472.0
4	95265.0
5	72233.0
6	49201.0
7	49450.0
8	49699.0
9	50433.0
10	51167.0
11	50406.0
12	49645.0
13	48571.5
14	47498.0
15	45031.0
16	42564.0
17	39904.0
18	37244.0
19	36115.5
20	34987.0
21	34395.5
22	33804.0
23	36208.5
24	38613.0
25	42372.0
26	46131.0
27	54319.5
28	62508.0
29	72016.0
30	81524.0
31	92073.5
32	102623.0
33	119176.0
34	135729.0
35	148514.5
36	161300.0
37	175048.0
38	188796.0
39	203112.5
40	217429.0
41	231944.0
42	246459.0
43	261694.5
44	276930.0
45	279978.0
46	283026.0
47	280580.0
48	278134.0
49	270419.5
50	262705.0
51	241742.5
52	220780.0
53	203114.0
54	185448.0
55	166240.5
56	147033.0
57	134379.0
58	121725.0
59	110278.5
60	98832.0
61	83287.0
62	67742.0
63	56215.5
64	44689.0
65	34134.5
66	23580.0
67	18411.5
68	13243.0
69	10446.5
70	7650.0
71	6222.5
72	4795.0
73	3885.5
74	2976.0
75	2426.5
76	1877.0
77	1559.5
78	1242.0
79	906.0
80	570.0
81	373.5
82	177.0
83	114.5
84	52.0
85	41.0
86	30.0
87	20.5
88	11.0
89	10.0
90	9.0
91	5.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3820818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.526386580363273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93538928744587	21.728060403977008
2	5.7855424911595374	3.185101583775508
3	2.412883271918218	1.99253873148338
4	1.5126379111745774	1.665498235964185
5	1.1320306445913504	1.5580356571919665
6	0.8864324802016296	1.464016987645216
7	0.6929721155956471	1.3352512840308575
8	0.5904416126080426	1.300217926542566
9	0.5136828795176771	1.2725850169185977
>10	6.458225725968101	41.5523737796941
>50	1.001318287121685	18.022282887417035
>100	0.0760228204019583	2.9760547878745696
>500	0.0017427400530068558	0.3373450979012542
>1k	5.809133508869002E-4	0.2501971700612527
>5k	0.0	0.0
>10k+	9.681889181448337E-5	1.3604404495224505
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51047	1.3360228097752889	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020519166314647806	0.0
2	0.0	0.0	0.0	0.08592400894258768	0.0
3	0.0	0.0	0.0	0.1688905360056407	0.0
4	0.0	0.0	0.0	0.2270717945738321	0.0
5	0.0	0.0	0.0	0.3481715171986732	0.0
6	0.0	0.0	0.0	0.6240810213938481	0.0
7	0.0	0.0	0.0	0.7887839724373158	0.0
8	0.0	0.0	0.0	1.1125628072313312	0.0
9	0.0	0.0	0.0	1.3120227134608349	0.0
10	0.0	0.0	0.0	1.5513170216430094	0.0
11	0.0	0.0	0.0	1.6887221532143117	0.0
12	0.0	0.0	0.0	1.8306551110259635	0.0
13	0.0	0.0	0.0	1.9240120832764083	0.0
14	0.0	0.0	0.0	1.9847058928219037	0.0
15	0.0	0.0	0.0	2.032496706202703	0.0
16	0.0	0.0	0.0	2.1049419260482964	0.0
17	0.0	0.0	0.0	2.181810282510185	0.0
18	0.0	0.0	0.0	2.307280796939294	0.0
19	0.0	0.0	0.0	2.3669015378382325	0.0
20	0.0	0.0	0.0	2.43623224136821	0.0
21	0.0	0.0	0.0	2.5066360135447434	0.0
22	0.0	0.0	0.0	2.586252472638058	0.0
23	0.0	0.0	0.0	2.673930032783556	0.0
24	0.0	0.0	0.0	2.7494897689447653	0.0
25	0.0	0.0	0.0	2.8199458859333264	0.0
26	0.0	0.0	0.0	2.889171899839249	0.0
27	0.0	0.0	0.0	2.9714841167519626	0.0
28	0.0	0.0	0.0	3.0535607820105537	0.0
29	0.0	0.0	0.0	3.1413953765921327	0.0
30	0.0	0.0	0.0	3.2602704447058195	0.0
31	0.0	0.0	0.0	3.3554333129712015	0.0
32	0.0	0.0	0.0	3.459442454469174	0.0
33	0.0	0.0	0.0	3.559473390253082	0.0
34	2.6172406013581384E-5	0.0	0.0	3.664634117615652	0.0
35	2.6172406013581384E-5	5.496205262852091E-4	0.0	3.772857016481811	0.0
36	2.6172406013581384E-5	5.496205262852091E-4	0.0	3.876499744295593	0.0
37	2.6172406013581384E-5	5.496205262852091E-4	0.0	3.987601607823246	0.0
38	5.234481202716277E-5	5.496205262852091E-4	0.0	4.10155626360638	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	46540	0.0	43.116028	1
CGACGGT	145	0.0	42.482758	28
GTTTTTT	51245	0.0	38.43185	2
TAGGGCG	1610	0.0	38.260868	5
CGTTCGA	70	0.0	37.714287	14
AGGGCGA	4025	0.0	37.714287	6
GACCGAT	2505	0.0	36.88623	9
TAGGGAC	4325	0.0	36.62428	5
TAGGGAT	7415	0.0	36.523266	5
GGGCGAT	7655	0.0	36.4128	7
CGCCGGT	315	0.0	36.317463	28
CGGTCTA	165	0.0	36.0	31
AGGGATC	5400	0.0	35.89259	6
ACGGGAT	2215	0.0	35.85553	5
TACGGGA	1580	0.0	35.78481	4
GCGATCG	345	0.0	35.710148	9
CGTAAGG	500	0.0	35.64	2
TAAGGGA	6150	0.0	35.62927	4
GGACCGA	2615	0.0	35.50287	8
GTAGGGA	5885	0.0	35.40187	4
>>END_MODULE