FastQCFastQC Report
Thu 26 May 2016
SRR1547189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547189_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences983492
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88160.8963977337893954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCC207.8559277E-444.016
CACGACG302.527342E-644.026
AATTGCG207.8559277E-444.01
TACGACG302.527342E-644.01
GTCGGGC254.4423694E-544.04
CGTTTTT92600.042.9308851
GCGTTAG800.041.251
GCGTAAG501.3478711E-939.6000021
AGTACGG1300.038.923082
AGGGCGA12350.038.8340076
TGCGCGC404.12374E-738.515
CCGACGG404.12374E-738.52
TACGGGA5400.038.29634
TAGGGCG4500.038.1333355
TGGGCGA5850.037.9829066
ACGGGAC3650.037.9726035
GTTTTTT103750.037.7869872
AAGCGCG357.2891344E-637.7142871
GACGACG357.2891344E-637.7142871
GTACGGG3700.037.4594573
Produced by FastQC (version 0.11.5)