FastQCFastQC Report
Thu 26 May 2016
SRR1547188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547188_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2784061
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233800.8397804502128365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT167650.041.9528771
CGAATAT2050.036.4878114
TACGGGA8350.036.359284
ATTAACG850.036.2352941
GGGCGAT61150.036.192977
ACGGGAT9950.036.04025
GTTTTTT200150.035.800152
TAGGGAT26150.035.671135
AGGGCGA31900.035.6551746
GTATGCG1050.035.6190451
ATAGGGC12500.035.5520024
AATGCGG4100.035.4146352
GTTGATC17050.035.3548416
CGGTCTA3800.035.3157931
GGCGATA11800.035.2372868
GCGCGAC11900.035.126059
CTCACGA4200.035.09523824
TACGAAT2200.035.012
GACCGAT19300.034.994829
TAAGGGA25650.034.9941524
Produced by FastQC (version 0.11.5)