##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547188_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2784061
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.550884481338592	31.0	30.0	31.0	27.0	33.0
2	30.88359594132456	31.0	31.0	33.0	28.0	34.0
3	31.027532442715874	31.0	31.0	34.0	28.0	34.0
4	34.87533929752258	35.0	35.0	37.0	32.0	37.0
5	34.68129685376865	35.0	35.0	37.0	32.0	37.0
6	34.7062140520628	35.0	35.0	37.0	31.0	37.0
7	35.33547468967095	35.0	35.0	37.0	33.0	37.0
8	34.30244811446301	35.0	35.0	37.0	32.0	37.0
9	36.38282530447429	39.0	37.0	39.0	32.0	39.0
10	35.8148086554138	37.0	35.0	39.0	30.0	39.0
11	35.58806937060646	37.0	35.0	39.0	30.0	39.0
12	35.21373418183007	37.0	34.0	39.0	30.0	39.0
13	35.080365696010254	37.0	34.0	39.0	29.0	39.0
14	35.94858302314497	38.0	34.0	40.0	28.0	41.0
15	36.094851010807595	38.0	34.0	40.0	30.0	41.0
16	36.15238243702275	38.0	34.0	40.0	30.0	41.0
17	36.04529642130686	38.0	34.0	40.0	29.0	41.0
18	35.92619522345236	38.0	34.0	40.0	29.0	41.0
19	35.88999307127251	37.0	34.0	40.0	29.0	41.0
20	35.73068047000407	37.0	34.0	40.0	29.0	41.0
21	35.56132857721149	37.0	34.0	40.0	29.0	41.0
22	35.52582432640664	37.0	34.0	40.0	28.0	41.0
23	35.40249189942318	37.0	34.0	40.0	28.0	41.0
24	35.28126359300317	37.0	33.0	40.0	27.0	41.0
25	35.07167694960707	37.0	33.0	40.0	27.0	41.0
26	34.88102487696929	37.0	33.0	40.0	26.0	41.0
27	34.65922190641656	36.0	33.0	40.0	25.0	41.0
28	34.664006643532595	36.0	33.0	40.0	25.0	41.0
29	34.43379724797697	36.0	33.0	40.0	25.0	41.0
30	34.41504263017226	36.0	33.0	40.0	25.0	41.0
31	34.34384735104583	36.0	33.0	40.0	24.0	41.0
32	34.074151033328654	36.0	32.0	40.0	23.0	41.0
33	33.87267987303439	36.0	32.0	40.0	23.0	41.0
34	33.62666586687576	36.0	32.0	40.0	22.0	41.0
35	33.40278679238709	36.0	31.0	40.0	21.0	41.0
36	33.343134722982	36.0	31.0	40.0	21.0	41.0
37	33.361286624107734	36.0	31.0	40.0	21.0	41.0
38	33.34024577766076	36.0	31.0	40.0	21.0	41.0
39	33.216589722710815	36.0	31.0	40.0	20.0	41.0
40	33.11668206982534	35.0	31.0	40.0	19.0	41.0
41	33.102765348891424	35.0	31.0	40.0	19.0	41.0
42	32.95189580975417	35.0	31.0	39.0	18.0	41.0
43	32.97416471837363	35.0	31.0	39.0	19.0	41.0
44	32.656772606634696	35.0	31.0	39.0	18.0	41.0
45	32.573134712206375	35.0	31.0	39.0	18.0	41.0
46	32.504671054262104	35.0	30.0	39.0	18.0	41.0
47	32.42263513622726	35.0	30.0	39.0	18.0	40.0
48	32.39942264196079	35.0	30.0	39.0	18.0	40.0
49	32.37890800524845	35.0	30.0	39.0	18.0	40.0
50	31.839535125128364	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	2.0
12	6.0
13	13.0
14	59.0
15	185.0
16	547.0
17	1365.0
18	2969.0
19	5680.0
20	9355.0
21	14376.0
22	20461.0
23	28339.0
24	39030.0
25	51746.0
26	67083.0
27	80420.0
28	88901.0
29	98630.0
30	113580.0
31	132386.0
32	155783.0
33	187869.0
34	247689.0
35	266149.0
36	255927.0
37	292528.0
38	328838.0
39	294142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.33569199812791	23.612485502293232	26.425534497986934	16.62628800159192
2	33.945843859024635	24.761454580197775	27.661857983715155	13.630843577062429
3	29.101876718936833	25.34682250137479	31.90332395734145	13.647976822346925
4	25.6744374494668	27.878843171898893	31.370397415861216	15.076321962773086
5	22.59523049243533	31.96941446326068	30.480833573689658	14.95452147061433
6	21.157905663704927	40.39818093066208	28.143851733133722	10.300061672499274
7	85.56349878828085	3.768415993758757	9.029902721240662	1.6381824967197196
8	83.59971279364927	5.968475547051591	8.61611868418113	1.815692975118002
9	80.4415564170469	5.4099030157744386	10.760935195026258	3.387605372152406
10	39.946754040231156	29.80207689414851	17.07437444797366	13.176794617646667
11	33.45174548977196	24.890941685544966	25.309359241769485	16.347953582913593
12	30.61703748588842	21.605597003801282	29.66874648220711	18.108619028103192
13	25.11062078021997	25.15835680324533	30.791530789016473	18.93949162751822
14	21.580525714055835	27.301521051442478	31.909537901647987	19.208415332853697
15	20.980251510293776	22.94640814263768	36.99997952631067	19.073360820757877
16	24.069372043213132	21.353878381256735	34.61522574397616	19.96152383155398
17	23.766648791100483	20.86875970030829	30.78937566382346	24.57521584476777
18	25.070032589084796	22.136979038893184	32.42410996023435	20.368878411787673
19	28.106029285996247	24.16789718328729	27.960773847986808	19.765299682729655
20	28.973143907407202	24.148896162835513	27.159498301222566	19.71846162853472
21	26.985256429367027	24.6197191800036	29.219546554475638	19.175477836153735
22	25.39014051775446	22.785312534459553	28.97138388849957	22.853163059286416
23	24.090169001325762	24.905883886883228	28.940206410707237	22.063740701083777
24	23.94584745089996	23.80274713808354	31.949946499017084	20.301458911999415
25	24.637139775313834	23.74556448296212	29.71342941120902	21.90386633051503
26	22.217329289839554	25.58553853525479	28.870021166921273	23.32711100798438
27	21.886230222685494	24.619503667484295	30.34786234928042	23.146403760549784
28	21.558579355840262	25.358136908638134	31.417738332601193	21.66554540292041
29	23.032613150358415	24.531466803349495	30.889804497818112	21.546115548473974
30	23.711657179925297	24.74611727257413	30.71401811957425	20.82820742792633
31	27.010327719112475	23.936903681348937	27.85179635072651	21.20097224881208
32	27.356620418877313	24.171560896115423	27.82712016726645	20.64469851774081
33	27.339702686112123	24.235963220633455	26.95587488923554	21.468459204018878
34	23.36428691756395	25.740563874139248	29.141638778748025	21.75351042954878
35	23.00183077885147	26.36030604214491	29.274969190689426	21.362893988314195
36	26.423343454040698	27.31987553433635	25.81865124363295	20.43812976799
37	24.073861887365254	29.11545400765285	25.913656345891845	20.897027759090047
38	24.392604903412675	28.957231899732083	25.88000047412754	20.770162722727697
39	24.159815463813473	27.54558897955181	25.42257515190939	22.872020404725326
40	25.05975982566474	24.922729782141985	27.50970614508806	22.507804247105216
41	21.882207322325193	25.159111097062887	28.049565005939165	24.909116574672755
42	23.26360665229677	26.25542328275135	26.135670159525958	24.345299905425925
43	22.710852959040768	24.842020343663446	27.64138429438148	24.805742402914305
44	22.46516868703667	25.554001869930293	28.289250846156026	23.691578596877008
45	22.50410461552387	27.140030336978967	27.090175107513808	23.265689939983357
46	23.1813527074299	27.277024461748507	27.706469075210638	21.83515375561096
47	22.61613520680761	26.39852359556777	27.972878467820927	23.012462729803694
48	22.8220215002473	25.390176436507677	29.29917843035767	22.488623632887354
49	23.59416693815258	25.05372547512429	29.335671883626112	22.016435703097024
50	22.590884323296077	26.537349576751375	27.912930068701797	22.95883603125075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1407.0
1	2683.0
2	3959.0
3	22923.0
4	41887.0
5	30186.5
6	18486.0
7	18487.5
8	18489.0
9	18693.5
10	18898.0
11	18691.5
12	18485.0
13	18223.0
14	17961.0
15	17007.0
16	16053.0
17	15328.0
18	14603.0
19	14418.5
20	14234.0
21	14156.5
22	14079.0
23	15273.0
24	16467.0
25	18458.5
26	20450.0
27	22425.5
28	24401.0
29	29345.5
30	34290.0
31	40183.5
32	46077.0
33	53564.5
34	61052.0
35	69604.0
36	78156.0
37	84333.5
38	90511.0
39	99725.0
40	108939.0
41	122058.5
42	135178.0
43	148708.5
44	162239.0
45	167059.5
46	171880.0
47	176861.5
48	181843.0
49	186439.0
50	191035.0
51	180809.0
52	170583.0
53	163572.5
54	156562.0
55	154461.5
56	152361.0
57	152856.0
58	153351.0
59	151618.0
60	149885.0
61	141204.0
62	132523.0
63	120675.5
64	108828.0
65	92561.5
66	76295.0
67	64762.0
68	53229.0
69	46783.5
70	40338.0
71	34009.0
72	27680.0
73	22877.0
74	18074.0
75	14428.0
76	10782.0
77	8639.0
78	6496.0
79	4891.5
80	3287.0
81	2450.5
82	1614.0
83	1141.0
84	668.0
85	475.0
86	282.0
87	185.5
88	89.0
89	67.0
90	45.0
91	33.5
92	22.0
93	14.0
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2784061.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.900868783357463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97647593625857	19.914837330441205
2	5.48166627140941	2.729965050770442
3	2.0326816281591804	1.5184661550339948
4	1.1472466712078593	1.1426975528756218
5	0.8491684043458708	1.0572515505794782
6	0.6787653060098834	1.0141107491788548
7	0.5431289526031112	0.9467067946848713
8	0.4562116252431685	0.908805265409791
9	0.4258068216173046	0.9542663812935899
>10	6.977770659423915	43.162294594068356
>50	1.3347200769289091	21.68533668662482
>100	0.09252799267562835	3.2977889530293245
>500	0.0027985933934199588	0.4918830597370162
>1k	8.837663347641977E-4	0.3180679959763928
>5k	0.0	0.0
>10k+	1.4729438912736628E-4	0.8575218802961329
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23380	0.8397804502128365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012356051106638828	0.0
2	0.0	0.0	0.0	0.052369542190347125	0.0
3	0.0	0.0	0.0	0.09486142724602657	0.0
4	0.0	0.0	0.0	0.1464048381123833	0.0
5	0.0	0.0	0.0	0.24277485299352278	0.0
6	0.0	0.0	0.0	0.4649682603937198	0.0
7	0.0	0.0	0.0	0.5898577653291361	0.0
8	0.0	0.0	0.0	0.907918325065435	0.0
9	0.0	0.0	0.0	1.0826271407127932	0.0
10	0.0	0.0	0.0	1.3231750310068637	0.0
11	0.0	0.0	0.0	1.4676043376923136	0.0
12	0.0	0.0	0.0	1.59331997395172	0.0
13	0.0	0.0	0.0	1.6642595115552425	0.0
14	0.0	0.0	0.0	1.7022974712120174	0.0
15	0.0	0.0	0.0	1.7357737492102363	0.0
16	0.0	0.0	0.0	1.7946445857328557	0.0
17	0.0	0.0	0.0	1.8557064662017104	0.0
18	0.0	0.0	0.0	1.9569255127671412	0.0
19	0.0	0.0	0.0	2.0026141668591313	0.0
20	0.0	0.0	0.0	2.0576416967875346	0.0
21	0.0	0.0	0.0	2.1152194581943426	0.0
22	0.0	0.0	0.0	2.1812740453603565	0.0
23	0.0	0.0	0.0	2.2572422084142554	0.0
24	0.0	0.0	0.0	2.3199204327778737	0.0
25	0.0	0.0	0.0	2.3755945002641825	0.0
26	0.0	0.0	0.0	2.4299036551282462	0.0
27	0.0	0.0	0.0	2.490965535597101	0.0
28	0.0	0.0	0.0	2.549872290872937	0.0
29	0.0	0.0	0.0	2.620057534658903	0.0
30	0.0	0.0	0.0	2.7124764866861755	0.0
31	0.0	0.0	0.0	2.7860380932745366	0.0
32	0.0	0.0	0.0	2.8685434694139245	0.0
33	0.0	0.0	0.0	2.9491092328795956	0.0
34	0.0	0.0	0.0	3.0369665032483124	0.0
35	0.0	0.0	0.0	3.1378622810347907	0.0
36	0.0	0.0	0.0	3.2252885263649036	0.0
37	0.0	0.0	0.0	3.319251984780506	0.0
38	0.0	0.0	0.0	3.429594394663048	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	16765	0.0	41.952877	1
CGAATAT	205	0.0	36.48781	14
TACGGGA	835	0.0	36.35928	4
ATTAACG	85	0.0	36.235294	1
GGGCGAT	6115	0.0	36.19297	7
ACGGGAT	995	0.0	36.0402	5
GTTTTTT	20015	0.0	35.80015	2
TAGGGAT	2615	0.0	35.67113	5
AGGGCGA	3190	0.0	35.655174	6
GTATGCG	105	0.0	35.619045	1
ATAGGGC	1250	0.0	35.552002	4
AATGCGG	410	0.0	35.414635	2
GTTGATC	1705	0.0	35.35484	16
CGGTCTA	380	0.0	35.31579	31
GGCGATA	1180	0.0	35.237286	8
GCGCGAC	1190	0.0	35.12605	9
CTCACGA	420	0.0	35.095238	24
TACGAAT	220	0.0	35.0	12
GACCGAT	1930	0.0	34.99482	9
TAAGGGA	2565	0.0	34.994152	4
>>END_MODULE