FastQCFastQC Report
Thu 26 May 2016
SRR1547187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547187_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2101146
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207760.9887937344668101No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA21420.10194436750230589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC207.857971E-444.00000412
TACGATC207.857971E-444.00000411
CGTTTTT157050.042.234961
TCGATAG1300.038.9230771
TATACTA16950.037.12094544
GTTTTTT180150.036.917012
AGGGCGA24100.036.6970986
GTTGATC10300.036.52427316
CGTCATA2600.036.38461338
GGGCGAT47400.035.877647
AAGGGAT25150.035.6898575
CATACGA3950.035.6455718
CGCATCG1250.035.221
TCGATCG250.002353187235.212
ATAGGGC9950.034.9346734
CGACCCG8250.034.93333432
ATTATAC18150.034.90909242
CGGTAGT5550.034.88288512
GCGATAC1200.034.8333329
TAGCATA10600.034.66037830
Produced by FastQC (version 0.11.5)