##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547187_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2101146
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.63683770666103	31.0	30.0	31.0	28.0	33.0
2	30.97569136080977	31.0	31.0	33.0	28.0	34.0
3	31.097079403335133	31.0	31.0	34.0	28.0	34.0
4	34.94959417384608	35.0	35.0	37.0	32.0	37.0
5	34.77400332961155	35.0	35.0	37.0	32.0	37.0
6	34.79889974328295	35.0	35.0	37.0	32.0	37.0
7	35.40481527699646	35.0	35.0	37.0	33.0	37.0
8	34.35891032798292	35.0	35.0	37.0	32.0	37.0
9	36.46221728523387	39.0	37.0	39.0	32.0	39.0
10	35.929405667193045	38.0	35.0	39.0	30.0	39.0
11	35.73048279367545	37.0	35.0	39.0	30.0	39.0
12	35.3945880010242	37.0	35.0	39.0	30.0	39.0
13	35.27846280077634	37.0	34.0	39.0	30.0	39.0
14	36.190031535171755	38.0	35.0	40.0	30.0	41.0
15	36.32214134572276	38.0	35.0	40.0	30.0	41.0
16	36.36395662176736	38.0	35.0	40.0	30.0	41.0
17	36.25089498778286	38.0	34.0	40.0	30.0	41.0
18	36.12869833890648	38.0	34.0	40.0	30.0	41.0
19	36.05391771918753	38.0	34.0	40.0	30.0	41.0
20	35.87805749814625	38.0	34.0	40.0	30.0	41.0
21	35.71599070221679	37.0	34.0	40.0	29.0	41.0
22	35.664890017162065	37.0	34.0	40.0	29.0	41.0
23	35.54561939056115	37.0	34.0	40.0	29.0	41.0
24	35.42334135752584	37.0	34.0	40.0	28.0	41.0
25	35.21677170458407	37.0	33.0	40.0	27.0	41.0
26	35.031927814630684	37.0	33.0	40.0	27.0	41.0
27	34.85224111032741	36.0	33.0	40.0	26.0	41.0
28	34.83011270992116	36.0	33.0	40.0	26.0	41.0
29	34.62816624832353	36.0	33.0	40.0	25.0	41.0
30	34.575225614973924	36.0	33.0	40.0	25.0	41.0
31	34.479460732381284	36.0	33.0	40.0	25.0	41.0
32	34.17268671477375	36.0	33.0	40.0	24.0	41.0
33	33.90858655229099	36.0	32.0	40.0	23.0	41.0
34	33.60447108387518	36.0	32.0	40.0	21.0	41.0
35	33.38097162215286	36.0	32.0	40.0	20.0	41.0
36	33.290688034053794	36.0	31.0	40.0	19.0	41.0
37	33.29867129652104	36.0	31.0	40.0	19.0	41.0
38	33.27865460087019	36.0	31.0	40.0	19.0	41.0
39	33.16713355473632	36.0	31.0	40.0	18.0	41.0
40	33.068257988735674	36.0	31.0	40.0	18.0	41.0
41	33.05068043819897	36.0	31.0	40.0	18.0	41.0
42	32.889173812766934	35.0	31.0	40.0	18.0	41.0
43	32.90843282665745	35.0	31.0	40.0	18.0	41.0
44	32.602202321970964	35.0	31.0	39.0	18.0	41.0
45	32.5244485628319	35.0	31.0	39.0	18.0	41.0
46	32.45371287859102	35.0	30.0	39.0	18.0	41.0
47	32.37808510212998	35.0	30.0	39.0	18.0	41.0
48	32.374145347348545	35.0	30.0	39.0	18.0	41.0
49	32.3429461827022	35.0	30.0	39.0	18.0	40.0
50	31.797113099232515	35.0	30.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	5.0
12	4.0
13	19.0
14	39.0
15	138.0
16	357.0
17	913.0
18	2069.0
19	4057.0
20	6822.0
21	10372.0
22	15006.0
23	21045.0
24	28757.0
25	39655.0
26	51634.0
27	60606.0
28	65801.0
29	71671.0
30	81992.0
31	96429.0
32	114105.0
33	139724.0
34	184488.0
35	201709.0
36	194651.0
37	223883.0
38	254220.0
39	230971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.55528173672843	23.385809458267058	25.992291825508556	18.06661697949595
2	35.86190583614846	24.4760240364068	26.569405457783514	13.092664669661222
3	28.362998097228846	25.069319314317045	33.08970438037147	13.477978208082636
4	25.363111368748292	27.537781762904622	32.47075643482176	14.628350433525325
5	22.5365586208669	30.988232136177114	31.911157054293227	14.564052188662757
6	20.879177363210363	39.642414187305405	29.61821786777311	9.860190581711123
7	84.59635836824285	3.6265447522447274	10.32051080695963	1.456586072552788
8	82.61358325409086	5.900637080907276	9.844056529151235	1.6417231358506263
9	79.50018704078632	5.510802200323061	11.950764011639363	3.0382467472512618
10	38.25064988344456	30.3466774798134	18.62145705248469	12.781215584257351
11	32.440630018094886	24.643694441033606	26.79923241887998	16.116443121991523
12	29.274215118797077	21.749702305313388	31.26846016411996	17.707622411769577
13	24.25209861665967	25.91557178796714	31.75367156780157	18.07865802757162
14	21.159262611927016	27.58009200693336	32.59664011924921	18.664005261890416
15	20.971745894859282	22.813550319682687	37.65721182630812	18.55749195914991
16	23.95402318544261	21.233936147226323	35.35042305484721	19.461617612483852
17	23.321939551083076	21.384711010086875	31.663244724545557	23.630104714284492
18	24.622515522481542	22.080950110082785	33.2910230893046	20.005511278131078
19	27.08050749448158	24.29550350142256	28.893565701764658	19.730423302331204
20	28.770632788011874	23.303521030904086	28.836644383588766	19.089201797495274
21	26.221024145870874	24.50843492075277	30.069400222545223	19.201140710831137
22	25.25588416987682	22.12663946246477	30.54423633579009	22.073240031868323
23	23.500413583825207	24.887846917824845	29.962077837522948	21.649661660826997
24	23.54643608773498	23.335598763722274	33.397488798969704	19.720476349573044
25	23.23498700233111	24.429097264064467	30.900613284369577	21.435302449234847
26	21.529060807768712	26.093474703804496	30.142836337884184	22.23462815054261
27	21.351015112705163	25.062085166856562	30.912416367068257	22.674483353370018
28	20.677715875051046	25.854129127628443	32.52839164912862	20.939763348191892
29	22.519853451402234	24.138969876438857	32.26410730144407	21.077069370714838
30	23.115385603856183	24.684481706649606	32.32807239477885	19.87206029471536
31	26.276850823312607	24.660447203573668	29.021257923057224	20.04144405005649
32	26.678631565821696	24.411440233091845	28.71061791993512	20.199310281151334
33	26.622709702229162	24.86800060538392	27.790691365569074	20.71859832681784
34	22.739685866665145	25.911383597332122	29.91662645051796	21.43230408548478
35	22.950332818376257	27.221145032282383	29.043769447720436	20.784752701620928
36	25.64038862601647	27.864222667058836	26.207745677834858	20.28764302908984
37	23.834374193892284	28.77415467559132	27.007166565293417	20.384304565222976
38	23.72609994736206	28.757163947674268	26.6762519120518	20.840484192911866
39	23.765602199942318	27.902773058131135	26.1954666643822	22.136158077544348
40	24.76710328554037	25.56433489153062	27.586755037489063	22.081806785439944
41	21.89048262234038	26.121982955967837	27.796640499993813	24.190893921697967
42	22.874326676965808	26.477788787642552	26.295031378114608	24.35285315727703
43	22.522185512096733	25.423744946805222	27.917764876881474	24.136304664216578
44	22.366698934771787	26.091570980788582	28.81175320515566	22.72997687928397
45	22.367127272450368	27.995056031327664	26.933302112275868	22.704514583946096
46	22.711748731406576	27.687747543483415	27.782790915053024	21.81771281005699
47	21.862640673232608	27.171219896189985	28.406783726594913	22.559355703982494
48	22.98883561637316	25.950552698384595	29.24975227804255	21.81085940719969
49	23.30261676247153	25.574757775042762	29.50570783753247	21.61691762495324
50	21.827136238985773	27.553439884710535	28.76354141977759	21.855882456526103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1099.0
1	2053.0
2	3007.0
3	19866.5
4	36726.0
5	26646.5
6	16567.0
7	16666.0
8	16765.0
9	17021.5
10	17278.0
11	17332.0
12	17386.0
13	16762.0
14	16138.0
15	15594.5
16	15051.0
17	14547.5
18	14044.0
19	13419.5
20	12795.0
21	12955.5
22	13116.0
23	13145.5
24	13175.0
25	14418.5
26	15662.0
27	18267.5
28	20873.0
29	23455.0
30	26037.0
31	30705.0
32	35373.0
33	41942.0
34	48511.0
35	54637.0
36	60763.0
37	66263.0
38	71763.0
39	79754.0
40	87745.0
41	96227.5
42	104710.0
43	111951.0
44	119192.0
45	123257.0
46	127322.0
47	131052.5
48	134783.0
49	139262.0
50	143741.0
51	138864.5
52	133988.0
53	128654.0
54	123320.0
55	117391.0
56	111462.0
57	110978.0
58	110494.0
59	106001.5
60	101509.0
61	96540.0
62	91571.0
63	82248.5
64	72926.0
65	62873.5
66	52821.0
67	45013.0
68	37205.0
69	32704.5
70	28204.0
71	23896.5
72	19589.0
73	16004.0
74	12419.0
75	9944.0
76	7469.0
77	5853.5
78	4238.0
79	3288.0
80	2338.0
81	1764.5
82	1191.0
83	809.5
84	428.0
85	289.5
86	151.0
87	128.5
88	106.0
89	88.5
90	71.0
91	45.5
92	20.0
93	11.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2101146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.74212428434618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.96574061060014	20.84969525329765
2	5.531605694462039	2.9585377394660184
3	2.3021394469729866	1.846922976325428
4	1.414604041421543	1.5131806835533317
5	1.0852627874135832	1.4511116171095007
6	0.8504754324944942	1.36461118299305
7	0.7220656560699146	1.3516698661255735
8	0.6170547418490379	1.3201083677417695
9	0.5544152186843113	1.334361661486994
>10	8.222362987646273	51.232543919269936
>50	0.6899253402459675	11.538986747810046
>100	0.041985279087935916	1.7466593928955252
>500	0.0018175100398807196	0.2909440191359796
>1k	3.635020079761439E-4	0.19086821970380755
>5k	0.0	0.0
>10k+	1.8175100398807196E-4	1.0097983530853847
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20776	0.9887937344668101	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	2142	0.10194436750230589	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00975658045656989	0.0
2	0.0	0.0	0.0	0.04145356867157256	0.0
3	0.0	0.0	0.0	0.0772435613707948	0.0
4	0.0	0.0	0.0	0.11013037647074501	0.0
5	0.0	0.0	0.0	0.1695741276427245	0.0
6	0.0	0.0	0.0	0.32506070496767003	0.0
7	0.0	0.0	0.0	0.4088245176679774	0.0
8	4.759307539790191E-5	0.0	0.0	0.6345108812048282	0.0
9	4.759307539790191E-5	0.0	0.0	0.7813355188073556	0.0
10	4.759307539790191E-5	0.0	0.0	0.9714222619465758	0.0
11	4.759307539790191E-5	0.0	0.0	1.084455816016593	0.0
12	4.759307539790191E-5	0.0	0.0	1.1854959150863387	0.0
13	4.759307539790191E-5	0.0	0.0	1.2430835363177999	0.0
14	4.759307539790191E-5	0.0	0.0	1.2806344728067445	0.0
15	4.759307539790191E-5	0.0	0.0	1.3088095734423024	0.0
16	4.759307539790191E-5	0.0	0.0	1.3520240859035975	0.0
17	4.759307539790191E-5	0.0	0.0	1.3971899144562063	0.0
18	4.759307539790191E-5	0.0	0.0	1.4725773458864828	0.0
19	4.759307539790191E-5	0.0	0.0	1.5085577108872967	0.0
20	4.759307539790191E-5	0.0	0.0	1.551058327217623	0.0
21	4.759307539790191E-5	0.0	0.0	1.5982706580123418	0.0
22	4.759307539790191E-5	0.0	0.0	1.649195248688097	0.0
23	4.759307539790191E-5	0.0	0.0	1.7048315538282441	0.0
24	4.759307539790191E-5	0.0	0.0	1.750996836964209	0.0
25	4.759307539790191E-5	0.0	0.0	1.793687825596127	0.0
26	4.759307539790191E-5	0.0	0.0	1.8402338533352751	0.0
27	4.759307539790191E-5	0.0	0.0	1.8914440024634176	0.0
28	4.759307539790191E-5	0.0	0.0	1.9409408008772355	0.0
29	4.759307539790191E-5	0.0	0.0	1.9949113483784564	0.0
30	4.759307539790191E-5	0.0	0.0	2.0796746156621198	0.0
31	4.759307539790191E-5	0.0	0.0	2.1414504275285964	0.0
32	4.759307539790191E-5	0.0	0.0	2.2122213306452765	0.0
33	4.759307539790191E-5	0.0	0.0	2.2786136708253495	0.0
34	4.759307539790191E-5	0.0	0.0	2.3486230847356633	0.0
35	4.759307539790191E-5	0.0	0.0	2.4275324037453845	0.0
36	4.759307539790191E-5	0.0	0.0	2.5003498091041747	0.0
37	4.759307539790191E-5	0.0	0.0	2.580734513451231	0.0
38	4.759307539790191E-5	0.0	0.0	2.681155902540804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	20	7.857971E-4	44.000004	12
TACGATC	20	7.857971E-4	44.000004	11
CGTTTTT	15705	0.0	42.23496	1
TCGATAG	130	0.0	38.923077	1
TATACTA	1695	0.0	37.120945	44
GTTTTTT	18015	0.0	36.91701	2
AGGGCGA	2410	0.0	36.697098	6
GTTGATC	1030	0.0	36.524273	16
CGTCATA	260	0.0	36.384613	38
GGGCGAT	4740	0.0	35.87764	7
AAGGGAT	2515	0.0	35.689857	5
CATACGA	395	0.0	35.64557	18
CGCATCG	125	0.0	35.2	21
TCGATCG	25	0.0023531872	35.2	12
ATAGGGC	995	0.0	34.934673	4
CGACCCG	825	0.0	34.933334	32
ATTATAC	1815	0.0	34.909092	42
CGGTAGT	555	0.0	34.882885	12
GCGATAC	120	0.0	34.833332	9
TAGCATA	1060	0.0	34.660378	30
>>END_MODULE