FastQCFastQC Report
Thu 26 May 2016
SRR1547186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547186_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2823637
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312011.1049933118173476No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA36020.12756597253825475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT218600.042.46021
CGGTCTA3400.040.1176531
CACGACG3700.037.45945726
TAGGGAC23000.037.2086945
CGAATAT5350.037.00934614
GTTTTTT253400.036.8981862
TCACGAC3900.036.66666825
CATATGC19300.036.59067533
GGGCGAT57600.036.43757
GCTACGA5500.036.410
AGGGCGA30750.036.2016266
TAGGGAT26200.036.1908385
GTTGATC19550.036.12276516
TTGATCC19600.036.03061317
TATACTA20500.035.95121844
TAGCATA19400.035.94845630
TAGGGTA12500.035.7285
TACGGGA7950.035.6981124
TACGAAT5550.035.67567412
TATAGCG1050.035.619051
Produced by FastQC (version 0.11.5)