##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547186_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2823637
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.533318907494127	31.0	30.0	31.0	27.0	33.0
2	30.873725624079867	31.0	31.0	33.0	28.0	34.0
3	31.00598164707432	31.0	31.0	34.0	28.0	34.0
4	34.84818515977798	35.0	35.0	37.0	32.0	37.0
5	34.65532502938586	35.0	35.0	37.0	31.0	37.0
6	34.69198909066569	35.0	35.0	37.0	31.0	37.0
7	35.334277033485535	35.0	35.0	37.0	33.0	37.0
8	34.295533030626814	35.0	35.0	37.0	32.0	37.0
9	36.38301984284807	39.0	37.0	39.0	32.0	39.0
10	35.80465229772807	37.0	35.0	39.0	30.0	39.0
11	35.59349555201324	37.0	35.0	39.0	30.0	39.0
12	35.2694220255649	37.0	34.0	39.0	30.0	39.0
13	35.17688463495839	37.0	34.0	39.0	29.0	39.0
14	36.05680616878161	38.0	34.0	40.0	29.0	41.0
15	36.18708495461704	38.0	34.0	40.0	30.0	41.0
16	36.22443890627584	38.0	34.0	40.0	30.0	41.0
17	36.118063688781525	38.0	34.0	40.0	30.0	41.0
18	35.992792274644366	38.0	34.0	40.0	29.0	41.0
19	35.909061965118035	37.0	34.0	40.0	29.0	41.0
20	35.719320153404986	37.0	34.0	40.0	29.0	41.0
21	35.54755126101549	37.0	34.0	40.0	29.0	41.0
22	35.51779956134588	37.0	34.0	40.0	28.0	41.0
23	35.37787116403419	37.0	33.0	40.0	28.0	41.0
24	35.243545115749654	37.0	33.0	40.0	27.0	41.0
25	35.01684246239867	37.0	33.0	40.0	27.0	41.0
26	34.82423696813719	36.0	33.0	40.0	26.0	41.0
27	34.60480614186597	36.0	33.0	40.0	25.0	41.0
28	34.5848659016722	36.0	33.0	40.0	25.0	41.0
29	34.34981550390507	36.0	33.0	40.0	25.0	41.0
30	34.31211979443533	36.0	33.0	40.0	24.0	41.0
31	34.215968979015365	36.0	33.0	40.0	24.0	41.0
32	33.90782809546695	36.0	32.0	40.0	23.0	41.0
33	33.63508198823008	36.0	32.0	40.0	22.0	41.0
34	33.384596886922786	36.0	31.0	40.0	21.0	41.0
35	33.1095084106066	36.0	31.0	40.0	19.0	41.0
36	33.02970034745967	36.0	31.0	40.0	18.0	41.0
37	33.03161206628189	36.0	31.0	40.0	18.0	41.0
38	33.00216989648457	36.0	31.0	40.0	18.0	41.0
39	32.860773180121946	35.0	31.0	40.0	18.0	41.0
40	32.7524561407858	35.0	31.0	39.0	18.0	41.0
41	32.73073521844345	35.0	31.0	39.0	18.0	41.0
42	32.5512234752555	35.0	31.0	39.0	17.0	41.0
43	32.557500131922055	35.0	31.0	39.0	18.0	41.0
44	32.2252842698973	35.0	30.0	39.0	16.0	41.0
45	32.14007041273365	35.0	30.0	39.0	17.0	41.0
46	32.076667078664855	35.0	30.0	39.0	17.0	40.0
47	31.986649842029976	35.0	30.0	39.0	17.0	40.0
48	31.963152841530267	35.0	30.0	39.0	17.0	40.0
49	31.958725572727655	35.0	30.0	39.0	17.0	40.0
50	31.425322730931775	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	2.0
11	3.0
12	10.0
13	26.0
14	57.0
15	231.0
16	623.0
17	1555.0
18	3436.0
19	6449.0
20	10466.0
21	16147.0
22	22654.0
23	31626.0
24	42664.0
25	57394.0
26	74807.0
27	88072.0
28	95006.0
29	102821.0
30	115005.0
31	133568.0
32	156533.0
33	187306.0
34	241109.0
35	263215.0
36	260640.0
37	295627.0
38	327276.0
39	289304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.9904304271406	22.50306962261792	25.777924003687442	18.728575946554034
2	35.65043948637874	24.307975848170287	26.847926982115617	13.193657683335358
3	28.76219570716774	25.025136021379517	32.88893012805825	13.323738143394495
4	24.952074222005166	27.54061517114275	32.71450969087032	14.792800915981763
5	21.88631187365798	31.299632353592195	32.281203284983164	14.532852487766664
6	20.347551756829933	40.2318003341081	29.599272144400995	9.821375764660967
7	84.35457532253615	3.4301859622890616	10.663516592253183	1.5517221229216078
8	82.12787266918517	5.755484858712363	10.18640143899517	1.9302410331073008
9	79.07252242409346	4.98190808521067	12.558094400944597	3.3874750897512675
10	38.802048563607855	28.269816552198456	19.082339550020063	13.845795334173621
11	32.25439388986616	24.660287423631296	26.53060574004378	16.554712946458768
12	28.93516411635065	20.890787307291976	31.39681198397669	18.777236592380678
13	24.634788395250524	23.65633401177276	32.359081567496105	19.34979602548061
14	21.580288117771513	25.869968413078592	32.71950325059489	19.830240218555005
15	21.12725538020645	22.20972455028745	36.63059380508189	20.032426264424217
16	24.292818092410602	20.457835054576776	34.588511200271135	20.660835652741483
17	23.580757724877525	21.289882516768266	31.799271648586558	23.33008810976765
18	24.57111165493298	21.663549528498173	33.10050123298427	20.664837583584575
19	27.226233400398137	24.020793041031833	28.6661139516163	20.086859606953727
20	28.435985220479825	23.61468559875083	28.01500334497671	19.93432583579263
21	26.30469143165357	24.494366662570293	29.935930149661587	19.265011756114543
22	25.34638836366006	21.770999600869377	30.0101252391862	22.872486796284367
23	24.00028757237563	24.301990659564243	29.375624416311304	22.322097351748827
24	23.984669417492405	23.261028241236392	32.41765850213749	20.336643839133714
25	24.44074787233628	23.464135085352687	30.040263674119583	22.05485336819145
26	22.546170063644865	24.466494807937423	29.55911117470128	23.428223953716433
27	21.9477928643094	23.491475710227625	30.978521672580435	23.58220975288254
28	22.24931887491204	24.025255370998465	31.61713067225001	22.108295081839486
29	23.297328941361798	23.72323354595509	31.16834777274841	21.8110897399347
30	23.98778596540561	23.62906421753221	31.60431032742523	20.778839489636947
31	26.30518724609431	23.73052910129737	29.0240211471942	20.94026250541412
32	26.757547092632656	23.75627603689851	27.83633306972532	21.64984380074351
33	26.260209793256	24.269018999255216	27.773152143848517	21.697619063640264
34	23.238929083306388	25.523181627100083	28.97486468692683	22.263024602666704
35	23.4071164246679	25.789823550265133	29.08716665775381	21.71589336731315
36	25.36770838461176	27.12554765361128	26.171352762412447	21.335391199364505
37	23.913059646123067	28.15128148554506	26.33787558386577	21.5977832844661
38	24.411494820332784	28.143667192348026	26.057882086118013	21.386955901201183
39	23.86712597972048	26.96748909296769	25.535541572801318	23.629843354510513
40	24.688017616995385	25.07879022693073	27.543625473104367	22.689566682969517
41	22.498996861140437	24.727434865034	27.78756617794709	24.986002095878472
42	23.07672693055092	25.858245943086878	26.141108081527477	24.92391904483473
43	22.46492732599835	24.760866924466566	28.182482379994312	24.591723369540773
44	22.91544557604253	24.52301765418147	28.88207655587457	23.679460213901432
45	22.42487260225022	26.421597393715977	27.583821858121283	23.569708145912525
46	23.386221387522546	26.0081589807755	28.10687067778188	22.49874895392007
47	22.322593166189563	26.393335970593956	28.294819766138495	22.98925109707799
48	22.49233878150768	25.341182312032316	29.16380540416491	23.00267350229509
49	23.343510514984754	24.405970030850284	29.082952235007546	23.16756721915742
50	22.645474613061097	25.55654285589826	28.772997378912375	23.024985152128266
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1595.0
1	2885.0
2	4175.0
3	28919.5
4	53664.0
5	38435.0
6	23206.0
7	23070.5
8	22935.0
9	23306.0
10	23677.0
11	23621.0
12	23565.0
13	22907.0
14	22249.0
15	21204.0
16	20159.0
17	18987.5
18	17816.0
19	17242.5
20	16669.0
21	16382.0
22	16095.0
23	16164.0
24	16233.0
25	17798.0
26	19363.0
27	21619.0
28	23875.0
29	27106.5
30	30338.0
31	35666.0
32	40994.0
33	47002.5
34	53011.0
35	62052.0
36	71093.0
37	76234.5
38	81376.0
39	90881.5
40	100387.0
41	112783.0
42	125179.0
43	138878.5
44	152578.0
45	162839.5
46	173101.0
47	178629.0
48	184157.0
49	183292.5
50	182428.0
51	175670.0
52	168912.0
53	162737.0
54	156562.0
55	156539.5
56	156517.0
57	158227.5
58	159938.0
59	156765.5
60	153593.0
61	147175.5
62	140758.0
63	128835.5
64	116913.0
65	99517.0
66	82121.0
67	72250.0
68	62379.0
69	54372.5
70	46366.0
71	38925.5
72	31485.0
73	26472.5
74	21460.0
75	17009.0
76	12558.0
77	9914.5
78	7271.0
79	5471.5
80	3672.0
81	2733.5
82	1795.0
83	1318.5
84	842.0
85	587.0
86	332.0
87	243.0
88	154.0
89	101.0
90	48.0
91	35.5
92	23.0
93	19.5
94	16.0
95	9.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2823637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.389300270848345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75929121554687	21.571618762218016
2	5.53785525829961	3.033559610521288
3	2.1681847345462355	1.781551882114694
4	1.3549912941199267	1.4844905367614423
5	0.9725438200155315	1.331864735648164
6	0.7814181150154463	1.2841497233543022
7	0.675383499551686	1.2948797027038246
8	0.5815192787245266	1.2741924910618567
9	0.5126023246919835	1.2635837091471271
>10	7.97381144722679	50.67298139212531
>50	0.6321506496721556	10.8890187566355
>100	0.04655823653587753	2.0812961169500035
>500	0.002635804323828769	0.5081653731709661
>1k	9.225315133400624E-4	0.4024028683129634
>5k	0.0	0.0
>10k+	1.3179021619143748E-4	1.126244339274558
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31201	1.1049933118173476	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	3602	0.12756597253825475	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016397291861524693	0.0
2	0.0	0.0	0.0	0.05538955609378968	0.0
3	0.0	0.0	0.0	0.10621053626935757	0.0
4	0.0	0.0	0.0	0.1634771041745097	0.0
5	0.0	0.0	0.0	0.2714938216208387	0.0
6	0.0	0.0	0.0	0.5564808790931696	0.0
7	0.0	0.0	0.0	0.732388759603306	0.0
8	0.0	0.0	0.0	1.1754343777192324	0.0
9	0.0	0.0	0.0	1.4371535717941082	0.0
10	0.0	0.0	0.0	1.7677201424970703	0.0
11	0.0	0.0	0.0	1.9445134059370945	0.0
12	0.0	0.0	0.0	2.1027490431666678	0.0
13	0.0	0.0	0.0	2.2033639593191334	0.0
14	0.0	0.0	0.0	2.2642428895782283	0.0
15	0.0	0.0	0.0	2.3082641288522567	0.0
16	0.0	0.0	0.0	2.367513954520358	0.0
17	0.0	0.0	0.0	2.426940856774437	0.0
18	0.0	0.0	0.0	2.5322305947967108	0.0
19	0.0	0.0	0.0	2.5840786191709486	0.0
20	0.0	0.0	0.0	2.6414160177104917	0.0
21	0.0	0.0	0.0	2.7061198022267026	0.0
22	0.0	0.0	0.0	2.7748609329032026	0.0
23	0.0	0.0	0.0	2.8532704451740787	0.0
24	0.0	0.0	0.0	2.9207012091143443	0.0
25	0.0	0.0	0.0	2.9826780142065004	0.0
26	0.0	0.0	0.0	3.0407591344071494	0.0
27	0.0	0.0	0.0	3.106879531611181	0.0
28	0.0	0.0	0.0	3.1703437800255485	0.0
29	0.0	0.0	0.0	3.2413514910025616	0.0
30	0.0	0.0	0.0	3.3431351126224795	0.0
31	0.0	0.0	0.0	3.424873664709734	0.0
32	0.0	0.0	0.0	3.5192909003529844	0.0
33	0.0	0.0	0.0	3.607793778024583	0.0
34	0.0	0.0	0.0	3.6975716071152207	0.0
35	0.0	0.0	0.0	3.801586393718456	0.0
36	0.0	0.0	0.0	3.8930287427172825	0.0
37	0.0	0.0	0.0	3.992297876816319	0.0
38	0.0	0.0	0.0	4.11444530582366	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21860	0.0	42.4602	1
CGGTCTA	340	0.0	40.11765	31
CACGACG	370	0.0	37.459457	26
TAGGGAC	2300	0.0	37.208694	5
CGAATAT	535	0.0	37.009346	14
GTTTTTT	25340	0.0	36.898186	2
TCACGAC	390	0.0	36.666668	25
CATATGC	1930	0.0	36.590675	33
GGGCGAT	5760	0.0	36.4375	7
GCTACGA	550	0.0	36.4	10
AGGGCGA	3075	0.0	36.201626	6
TAGGGAT	2620	0.0	36.190838	5
GTTGATC	1955	0.0	36.122765	16
TTGATCC	1960	0.0	36.030613	17
TATACTA	2050	0.0	35.951218	44
TAGCATA	1940	0.0	35.948456	30
TAGGGTA	1250	0.0	35.728	5
TACGGGA	795	0.0	35.698112	4
TACGAAT	555	0.0	35.675674	12
TATAGCG	105	0.0	35.61905	1
>>END_MODULE