FastQCFastQC Report
Thu 26 May 2016
SRR1547185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547185_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5299099
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT552051.0417808763338825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT563100.043.1756321
GTTTTTT629400.038.120752
CGACGGT4300.037.86046628
AGGGCGA45000.037.846
TAGGGAT104700.037.381095
ACGGGAT28550.037.0647965
CGGTCTA4550.036.74725331
GGGCGAT93050.036.6706057
GACCGAT36250.036.595869
CGTTAGG7100.036.253522
GGCGATA19350.036.155048
AGGGATT116300.036.036116
CGTAAGG8100.035.8518522
CGAGGGA39050.035.6619724
TAGGGCG16500.035.65
AAGGGAT105500.035.5753565
CACGACG4700.035.57446726
TCTAGCG1550.035.483871
AGGGATC72250.035.38276
GGGACCG42750.035.1485377
Produced by FastQC (version 0.11.5)