##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547185_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5299099
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.581850424006042	31.0	30.0	31.0	27.0	34.0
2	30.927496542336726	31.0	31.0	33.0	28.0	34.0
3	31.018095717781456	31.0	31.0	34.0	28.0	34.0
4	34.85761711566438	35.0	35.0	37.0	32.0	37.0
5	34.67704679606854	35.0	35.0	37.0	32.0	37.0
6	34.7154821980114	35.0	35.0	37.0	31.0	37.0
7	35.4510074259794	35.0	35.0	37.0	35.0	37.0
8	34.43684954744193	36.0	35.0	37.0	33.0	37.0
9	36.53976194821044	39.0	37.0	39.0	32.0	39.0
10	35.90037438440007	38.0	35.0	39.0	30.0	39.0
11	35.65113597613481	37.0	35.0	39.0	30.0	39.0
12	35.49128616015666	37.0	35.0	39.0	30.0	39.0
13	35.401860391738296	37.0	35.0	39.0	30.0	39.0
14	36.36646361957004	38.0	35.0	40.0	30.0	41.0
15	36.51010936010065	38.0	35.0	40.0	30.0	41.0
16	36.55267376586095	38.0	35.0	40.0	30.0	41.0
17	36.46910880510064	38.0	35.0	40.0	30.0	41.0
18	36.299105187504516	38.0	35.0	40.0	30.0	41.0
19	36.18636413473309	37.0	34.0	40.0	30.0	41.0
20	35.9533947186116	37.0	34.0	40.0	30.0	41.0
21	35.79826759228314	37.0	34.0	40.0	29.0	41.0
22	35.74357055793825	37.0	34.0	40.0	29.0	41.0
23	35.62150754307478	37.0	34.0	40.0	29.0	41.0
24	35.49322573516743	37.0	34.0	40.0	29.0	41.0
25	35.30891157157094	37.0	34.0	40.0	27.0	41.0
26	35.150929431588274	37.0	33.0	40.0	27.0	41.0
27	34.96253646138712	37.0	33.0	40.0	27.0	41.0
28	34.96735407283389	37.0	33.0	40.0	27.0	41.0
29	34.809473082122075	36.0	33.0	40.0	26.0	41.0
30	34.74416801799702	37.0	33.0	40.0	25.0	41.0
31	34.59488547015257	37.0	33.0	40.0	25.0	41.0
32	34.16133459669276	37.0	33.0	40.0	23.0	41.0
33	33.79007808685967	37.0	33.0	40.0	21.0	41.0
34	33.50581655485206	37.0	32.0	40.0	18.0	41.0
35	33.19036236160147	37.0	31.0	40.0	18.0	41.0
36	33.04542979853745	37.0	31.0	40.0	16.0	41.0
37	33.00649242446688	37.0	31.0	40.0	15.0	41.0
38	33.018580517178485	37.0	31.0	40.0	15.0	41.0
39	32.90899773716249	37.0	31.0	40.0	15.0	41.0
40	32.877012110926785	37.0	31.0	40.0	15.0	41.0
41	32.89104411900966	37.0	31.0	40.0	15.0	41.0
42	32.73216258084629	36.0	31.0	40.0	15.0	41.0
43	32.78219693574323	36.0	31.0	40.0	15.0	41.0
44	32.49665103444944	36.0	30.0	40.0	13.0	41.0
45	32.444034542476	36.0	30.0	40.0	13.0	41.0
46	32.3968208557719	36.0	30.0	40.0	12.0	41.0
47	32.354051132088685	36.0	30.0	40.0	12.0	41.0
48	32.35132727280619	36.0	30.0	40.0	12.0	41.0
49	32.34808294768601	36.0	30.0	40.0	12.0	41.0
50	31.800945217290714	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	3.0
12	6.0
13	20.0
14	76.0
15	349.0
16	1057.0
17	2890.0
18	6544.0
19	12400.0
20	20126.0
21	29981.0
22	42294.0
23	58683.0
24	81279.0
25	113043.0
26	148829.0
27	166205.0
28	167427.0
29	173370.0
30	194138.0
31	226523.0
32	269258.0
33	322144.0
34	417605.0
35	478212.0
36	463961.0
37	544964.0
38	666635.0
39	691074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.90030946770385	22.415131327042577	27.14391635257239	20.54064285268118
2	35.47952208479215	24.443136465274566	27.612562060078517	12.464779389854765
3	25.26614430113497	24.955657556124162	37.161600490951386	12.616597651789482
4	22.62943190908492	27.57991500064445	36.20940088116867	13.581252209101963
5	20.225853489432826	31.499071823342046	35.51439593787548	12.760678749349655
6	18.549927072507987	39.634568065250335	32.69342580691548	9.122079055326198
7	83.52842624755642	2.749561765122712	12.617843146542459	1.104168840778404
8	81.96610404900908	4.952502302749958	11.886586757484622	1.1948068907563343
9	78.50087345037335	5.427205643827375	13.904967618079981	2.1669532877192896
10	32.84881071291553	36.45402737333271	20.536038296321696	10.161123617430057
11	24.55994877619761	24.892873297894607	35.27365689903132	15.273521026876455
12	24.210172333070208	23.413282144757062	35.882515121910345	16.494030400262385
13	19.907365384190783	28.68727306283578	36.17594236303191	15.229419189941535
14	17.78173987691115	31.06360156698337	34.922182053968044	16.232476502137438
15	16.545567463449917	26.010138704711878	41.93871448712319	15.505579344715018
16	18.50301343681256	24.290940780687436	40.626774476189254	16.57927130631075
17	19.170277815153106	23.861886709419846	35.43436346443046	21.533472010996586
18	19.695423693726045	24.806537866154226	37.50060151735229	17.997436922767438
19	22.41007386350019	26.073583452583165	33.84343262882992	17.672910055086724
20	23.639018633167638	25.56576127375616	33.602184069405006	17.193036023671194
21	21.182884109166483	26.670854800032988	35.11293146249957	17.03332962830096
22	21.024121270427294	24.27618355497793	34.766551823243915	19.933143351350864
23	18.18897891886904	28.090190426712162	34.251464258357885	19.469366396060916
24	17.900458172228902	26.05718443833565	38.93367532857944	17.108682060856005
25	17.80915963260924	27.54670180723176	36.6795940215497	17.9645445386093
26	17.897665244601015	29.790102053198098	33.97490780979936	18.33732489240152
27	17.942370957779804	28.81042607431943	35.29786856218387	17.949334405716897
28	17.120646358937623	27.5471924566799	37.92491893433204	17.407242250050434
29	17.578478907452002	25.265049775442954	37.91029380655089	19.24617751055415
30	18.17325926539587	27.510091809947312	35.94892263760311	18.367726287053703
31	21.057277850442123	26.67600661923848	33.628641397339436	18.638074132979966
32	21.385163779729346	26.702501689438147	33.40705655810544	18.50527797272706
33	21.001023004099377	27.88187199371063	32.410245590807044	18.706859411382954
34	18.297148251051734	28.18905629051278	33.099570323181354	20.414225135254124
35	19.40467615343665	27.891326431153672	32.612374292308935	20.09162312310074
36	22.3719730467387	27.540342235538535	30.920256443595413	19.167428274127357
37	19.56853419798347	30.08435962415497	31.774571488473796	18.572534689387762
38	19.71133205852542	30.075395836160073	30.99687324203605	19.216398863278457
39	19.431001383442734	30.205606651243922	30.973774975708135	19.389616989605212
40	21.21064354525175	28.379635858850722	30.5253968646368	19.884323731260732
41	18.844562066117277	27.643378619648356	31.31428569271871	22.19777362151566
42	20.01376082990712	28.832694010812027	29.843016709066955	21.310528450213894
43	19.891324921463063	28.660777992636106	30.58127428832713	20.8666227975737
44	19.68147792671924	29.693802663433917	31.20775814907402	19.416961260772823
45	18.80295121868831	31.203361175173367	29.397186200899434	20.59650140523889
46	19.949013973884995	30.778194557225675	29.980568394740313	19.29222307414902
47	19.845449198061786	28.918368952910672	30.56627173789356	20.669910111133987
48	20.376237545288358	27.72131639737246	31.31885250681295	20.58359355052623
49	19.92682152192288	27.513167804564514	31.872569280173856	20.687441393338755
50	19.15446380601683	29.846526739734436	30.895818326851415	20.10319112739732
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3271.0
1	4184.5
2	5098.0
3	51677.0
4	98256.0
5	76203.5
6	54151.0
7	55197.0
8	56243.0
9	57921.0
10	59599.0
11	59884.5
12	60170.0
13	59458.0
14	58746.0
15	56695.5
16	54645.0
17	53019.5
18	51394.0
19	50840.0
20	50286.0
21	49453.0
22	48620.0
23	49885.5
24	51151.0
25	61292.0
26	71433.0
27	79805.0
28	88177.0
29	100326.5
30	112476.0
31	131272.0
32	150068.0
33	169701.5
34	189335.0
35	209758.5
36	230182.0
37	253268.0
38	276354.0
39	297365.5
40	318377.0
41	348321.5
42	378266.0
43	401472.0
44	424678.0
45	431309.0
46	437940.0
47	425417.0
48	412894.0
49	397069.5
50	381245.0
51	346083.5
52	310922.0
53	277306.5
54	243691.0
55	214631.5
56	185572.0
57	163947.0
58	142322.0
59	123452.5
60	104583.0
61	88617.5
62	72652.0
63	59067.0
64	45482.0
65	37035.5
66	28589.0
67	22841.5
68	17094.0
69	13471.5
70	9849.0
71	8248.5
72	6648.0
73	5153.0
74	3658.0
75	2978.0
76	2298.0
77	1840.5
78	1383.0
79	1143.0
80	903.0
81	559.0
82	215.0
83	168.5
84	122.0
85	76.5
86	31.0
87	23.0
88	15.0
89	12.5
90	10.0
91	6.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5299099.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.96423618485113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17911954212236	20.51538949712658
2	6.232552520306913	3.1118182630286193
3	2.2429367818448576	1.6797961070899485
4	1.2099906917274215	1.20825973639019
5	0.8111503902090536	1.0124874961306485
6	0.6282146278132505	0.9409739006105002
7	0.47504744360100765	0.8301437606745713
8	0.4078418666258213	0.8145168547614046
9	0.3447713827563221	0.7746258806015882
>10	3.5588457053527573	20.24426411489864
>50	1.1648708979341946	21.16746218025782
>100	0.7416398202236317	25.934708316071887
>500	0.002012219660560281	0.31461644240993625
>1k	9.287167589233643E-4	0.3843444391569642
>5k	0.0	0.0
>10k+	7.73930632436137E-5	1.0665930107907409
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55205	1.0417808763338825	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022758585940742003	0.0
2	0.0	0.0	0.0	0.08537300397671378	0.0
3	0.0	0.0	0.0	0.15144083928230062	0.0
4	0.0	0.0	0.0	0.22852941603846239	0.0
5	0.0	0.0	0.0	0.3674398232605203	0.0
6	0.0	0.0	0.0	0.6922686290631671	0.0
7	0.0	0.0	0.0	0.8900003566644066	0.0
8	0.0	0.0	0.0	1.3465496681605684	0.0
9	0.0	0.0	0.0	1.5897042119801876	0.0
10	0.0	0.0	0.0	1.8942088079501818	0.0
11	0.0	0.0	0.0	2.076032170752047	0.0
12	0.0	0.0	0.0	2.2654983422653547	0.0
13	0.0	0.0	0.0	2.375120751659858	0.0
14	0.0	0.0	0.0	2.4304131702389404	0.0
15	0.0	0.0	0.0	2.4778174553825094	0.0
16	0.0	0.0	0.0	2.5598125266200915	0.0
17	0.0	0.0	0.0	2.653149148562803	0.0
18	0.0	0.0	0.0	2.8022310962674974	0.0
19	0.0	0.0	0.0	2.873299781717609	0.0
20	0.0	0.0	0.0	2.9532567706321395	0.0
21	0.0	0.0	0.0	3.043083361907373	0.0
22	0.0	0.0	0.0	3.1432702049914525	0.0
23	0.0	0.0	0.0	3.2637435156429424	0.0
24	0.0	0.0	0.0	3.358042565349317	0.0
25	0.0	0.0	0.0	3.4447742908747316	0.0
26	0.0	0.0	0.0	3.5252219292374045	0.0
27	0.0	0.0	0.0	3.614086092748975	0.0
28	0.0	0.0	0.0	3.7070264209066486	0.0
29	0.0	0.0	0.0	3.81261040792029	0.0
30	0.0	0.0	0.0	3.9492374080952253	0.0
31	0.0	0.0	0.0	4.065747780896337	0.0
32	0.0	0.0	0.0	4.177464886011754	0.0
33	0.0	0.0014153349465635572	0.0	4.293031702181824	0.0
34	0.0	0.0014153349465635572	0.0	4.411731126366954	0.0
35	0.0	0.0014153349465635572	0.0	4.541338065206935	0.0
36	0.0	0.0014153349465635572	0.0	4.6708883906490515	0.0
37	0.0	0.0014153349465635572	0.0	4.800740654213103	0.0
38	0.0	0.0014153349465635572	0.0	4.936726035878929	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	56310	0.0	43.175632	1
GTTTTTT	62940	0.0	38.12075	2
CGACGGT	430	0.0	37.860466	28
AGGGCGA	4500	0.0	37.84	6
TAGGGAT	10470	0.0	37.38109	5
ACGGGAT	2855	0.0	37.064796	5
CGGTCTA	455	0.0	36.747253	31
GGGCGAT	9305	0.0	36.670605	7
GACCGAT	3625	0.0	36.59586	9
CGTTAGG	710	0.0	36.25352	2
GGCGATA	1935	0.0	36.15504	8
AGGGATT	11630	0.0	36.03611	6
CGTAAGG	810	0.0	35.851852	2
CGAGGGA	3905	0.0	35.661972	4
TAGGGCG	1650	0.0	35.6	5
AAGGGAT	10550	0.0	35.575356	5
CACGACG	470	0.0	35.574467	26
TCTAGCG	155	0.0	35.48387	1
AGGGATC	7225	0.0	35.3827	6
GGGACCG	4275	0.0	35.148537	7
>>END_MODULE