FastQCFastQC Report
Thu 26 May 2016
SRR1547184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547184_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3275107
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225290.687885922505738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT159650.042.236141
GCGATAC2100.038.7619069
CACGACG4300.038.37209326
CGGTCTA4650.037.84946431
ACGGGTA4550.037.230775
GTTGATC19800.035.8888916
TTGATCC20000.035.7517
AGGGCGA34550.035.6584666
GGTACCT20550.035.6496358
GACCGAT21500.035.5069779
ATAACGC1550.035.4838711
GGGCGAT64750.035.4718137
CGTTAGG4900.035.4693872
TACGTCT250.00235337835.235
TTAGGGA30650.035.1712884
TAGCATA20100.035.1343330
CGGTAGT17200.035.04651312
GTTTTTT201150.034.845642
TGCCAGT20650.034.624724
CGACGGT4900.034.57142628
Produced by FastQC (version 0.11.5)