##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547184_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3275107
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.68860406698163	31.0	30.0	31.0	28.0	33.0
2	31.012963545923842	31.0	31.0	33.0	28.0	34.0
3	31.159199989496525	31.0	31.0	34.0	28.0	34.0
4	35.00088546725344	35.0	35.0	37.0	32.0	37.0
5	34.82594309132495	35.0	35.0	37.0	32.0	37.0
6	34.853649667018516	35.0	35.0	37.0	32.0	37.0
7	35.44891327214653	35.0	35.0	37.0	35.0	37.0
8	34.40576506355365	36.0	35.0	37.0	33.0	37.0
9	36.51318506540397	39.0	37.0	39.0	32.0	39.0
10	35.98761628246039	38.0	35.0	39.0	30.0	39.0
11	35.803165820231214	37.0	35.0	39.0	30.0	39.0
12	35.42551953264428	37.0	35.0	39.0	30.0	39.0
13	35.26723096375172	37.0	34.0	39.0	30.0	39.0
14	36.17794258324995	38.0	35.0	40.0	30.0	41.0
15	36.32573897585636	38.0	35.0	40.0	30.0	41.0
16	36.39526036859254	38.0	35.0	40.0	30.0	41.0
17	36.3063069389794	38.0	34.0	40.0	30.0	41.0
18	36.19033454479502	38.0	34.0	40.0	30.0	41.0
19	36.160652766459236	38.0	34.0	40.0	30.0	41.0
20	36.022191336038794	38.0	34.0	40.0	30.0	41.0
21	35.83797231662965	38.0	34.0	40.0	29.0	41.0
22	35.80589183803766	38.0	34.0	40.0	29.0	41.0
23	35.71823821328586	37.0	34.0	40.0	29.0	41.0
24	35.59453446864484	37.0	34.0	40.0	29.0	41.0
25	35.39533334330756	37.0	34.0	40.0	27.0	41.0
26	35.22640909136709	37.0	34.0	40.0	27.0	41.0
27	35.0415458181977	37.0	33.0	40.0	27.0	41.0
28	35.012375778867685	37.0	33.0	40.0	27.0	41.0
29	34.83799857531372	37.0	33.0	40.0	26.0	41.0
30	34.83783552720568	37.0	33.0	40.0	26.0	41.0
31	34.76809887432685	37.0	33.0	40.0	25.0	41.0
32	34.50932381751192	37.0	33.0	40.0	24.0	41.0
33	34.316311497609085	37.0	33.0	40.0	24.0	41.0
34	34.106619722653335	37.0	33.0	40.0	23.0	41.0
35	33.88939720137388	36.0	32.0	40.0	23.0	41.0
36	33.849971924581396	36.0	32.0	40.0	22.0	41.0
37	33.8644435128379	36.0	32.0	40.0	23.0	41.0
38	33.864718618353535	36.0	32.0	40.0	23.0	41.0
39	33.74980878487329	36.0	32.0	40.0	22.0	41.0
40	33.651466349038365	36.0	32.0	40.0	22.0	41.0
41	33.64187979201901	36.0	32.0	40.0	22.0	41.0
42	33.48279460793189	36.0	32.0	40.0	21.0	41.0
43	33.50377773917005	36.0	32.0	40.0	22.0	41.0
44	33.21237351939952	35.0	31.0	40.0	20.0	41.0
45	33.13455621449925	35.0	31.0	39.0	20.0	41.0
46	33.06971100486182	35.0	31.0	39.0	20.0	41.0
47	32.98565115582483	35.0	31.0	39.0	20.0	41.0
48	32.962456188454304	35.0	31.0	39.0	20.0	41.0
49	32.942209827037715	35.0	31.0	39.0	20.0	41.0
50	32.38704262181358	35.0	30.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	8.0
12	9.0
13	35.0
14	46.0
15	172.0
16	473.0
17	1193.0
18	2684.0
19	5111.0
20	8534.0
21	13474.0
22	19726.0
23	27734.0
24	38950.0
25	52481.0
26	68609.0
27	82471.0
28	92443.0
29	105201.0
30	123789.0
31	147715.0
32	177621.0
33	217986.0
34	295513.0
35	324558.0
36	307656.0
37	357804.0
38	412899.0
39	390210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.75556676468891	23.241042201063966	26.348482660261176	15.654908373985949
2	34.173875845888396	25.02458698295964	27.376998675157786	13.424538495994176
3	29.327438767649422	25.663558473051413	31.213087083872377	13.795915675426787
4	25.69778636239976	28.226314437971034	30.904486479373038	15.171412720256164
5	22.63309259819603	31.643363102335282	30.586634268742973	15.136910030725714
6	21.136591873181548	40.92599112029012	28.024061503944758	9.913355502583581
7	86.72562453684719	3.6137750613949406	8.137199792251062	1.523400609506804
8	84.468079974181	5.888174035229994	7.8332097241403105	1.8105362664486992
9	81.32839629361727	5.589191437104192	9.8699676071652	3.212444662113329
10	39.68774149974337	30.27085832615545	16.77606258360414	13.265337590497042
11	33.703570600899454	25.45147379917664	24.65223273621289	16.19272286371102
12	30.619915624130755	22.872321423391664	28.727244636587447	17.780518315890138
13	24.882820622349133	27.270498337916898	29.498150747441226	18.34853029229274
14	21.119401595123456	29.678511266960133	30.83019883014509	18.371888307771318
15	20.20056138623868	23.527536657580956	37.841847609864345	18.430054346316012
16	23.60188537351604	22.04025700534364	35.19414785532198	19.16370976581834
17	23.12532079104591	21.7684185585387	30.02301909525399	25.083241555161408
18	24.34696026725234	23.286750631353417	32.08982179818858	20.276467303205667
19	27.899943421695838	25.013869775857707	27.195477888203346	19.890708914243106
20	29.055814054319445	24.63898126076492	27.020430172205064	19.284774512710577
21	26.32784211325004	25.64426139359722	28.97102293146453	19.056873561688214
22	25.494922761302153	23.614342981771284	28.330280506865886	22.560453750060685
23	23.435325929809316	26.27340114384049	28.25880192616608	22.032471000184117
24	23.589214031785833	24.093930366244525	32.26416724705483	20.052688354914817
25	23.434135129020213	24.927948918920816	30.140603039839615	21.497312912219357
26	21.339486007632726	27.296726488630753	28.641873380014758	22.72191412372176
27	21.64365927586488	26.378130546574507	29.812796955946787	22.16541322161383
28	20.988993642039784	26.500508227670117	31.77633585711856	20.734162273171535
29	22.796659773253207	25.260029672312996	30.934989299586242	21.00832125484755
30	23.2902619670136	25.628658849924594	30.4848971346585	20.596182048403307
31	26.819032172078654	24.730581321465223	27.5399857164972	20.910400789958924
32	27.170074138035794	25.124797449365776	27.663004598017714	20.042123814580716
33	26.913685568135637	25.38408668785478	26.82000923939279	20.882218504616795
34	23.165533217693344	26.43422031707666	29.25299845165364	21.147248013576352
35	23.093718770104303	26.74593532363981	29.060485657415164	21.099860248840724
36	26.2870800862384	27.363014399224205	26.15471799852646	20.195187516010925
37	23.97973562390481	29.074714200177276	26.516843571828343	20.428706604089577
38	24.151913204667817	29.34185051053294	25.378651750919897	21.12758453387935
39	23.655593542439988	28.017069365977964	26.132825584019088	22.194511507562957
40	24.547533866832442	25.620903378118637	27.30289422605124	22.528668528997677
41	21.97677816327833	25.413032307036076	28.218100965861577	24.39208856382402
42	23.343237335451942	26.82123057353546	25.788897889443003	24.046634201569596
43	22.300614911207482	26.00855483500234	27.435347913823882	24.255482339966296
44	22.25298287964332	26.789933886129525	28.182957075906224	22.774126158320936
45	21.85733168412513	28.026259905401563	27.216454302103717	22.899954108369588
46	23.180464027587497	27.37880014301823	27.467927002079627	21.97280882731465
47	22.762767750794097	27.19923349069206	27.604960692887285	22.433038065626558
48	23.132923596084037	25.920313443194377	28.89780394961142	22.048959011110174
49	23.178448826252087	25.564752540909353	29.020364830828427	22.236433802010133
50	21.76301415495738	28.309945293390413	28.19071254771218	21.736328003940024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2058.0
1	3326.5
2	4595.0
3	23001.5
4	41408.0
5	30103.0
6	18798.0
7	18766.0
8	18734.0
9	19130.0
10	19526.0
11	19653.0
12	19780.0
13	19417.5
14	19055.0
15	18704.5
16	18354.0
17	17468.5
18	16583.0
19	16319.0
20	16055.0
21	16932.0
22	17809.0
23	19152.5
24	20496.0
25	22424.0
26	24352.0
27	29174.5
28	33997.0
29	39774.5
30	45552.0
31	51220.0
32	56888.0
33	67914.5
34	78941.0
35	86529.5
36	94118.0
37	104269.0
38	114420.0
39	128887.5
40	143355.0
41	161422.0
42	179489.0
43	193723.0
44	207957.0
45	215374.5
46	222792.0
47	224424.0
48	226056.0
49	226845.0
50	227634.0
51	219198.5
52	210763.0
53	199393.0
54	188023.0
55	179142.0
56	170261.0
57	166136.5
58	162012.0
59	157536.0
60	153060.0
61	146191.5
62	139323.0
63	126563.5
64	113804.0
65	98048.0
66	82292.0
67	68610.5
68	54929.0
69	47394.0
70	39859.0
71	34906.0
72	29953.0
73	24643.5
74	19334.0
75	14950.5
76	10567.0
77	8432.0
78	6297.0
79	4700.0
80	3103.0
81	2256.5
82	1410.0
83	1056.5
84	703.0
85	523.5
86	344.0
87	241.0
88	138.0
89	107.0
90	76.0
91	51.5
92	27.0
93	20.5
94	14.0
95	12.0
96	10.0
97	6.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3275107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.697794164309403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.14496288793454	17.60666702212929
2	5.6093664349375505	2.4342175659492202
3	1.9606526716686108	1.2762551429270648
4	1.0679305158657557	0.9268694606015969
5	0.7516170707615036	0.8154216245882142
6	0.5641132309568686	0.7344007662399399
7	0.46438436304554437	0.7053281425740305
8	0.38284917876951885	0.664558614153273
9	0.3330870458149547	0.6504528743001703
>10	5.212153077770139	28.47107851114618
>50	2.0349202806190236	31.051411119131096
>100	0.4700663531549974	13.223981436884557
>500	0.0024535965895047295	0.35597187233234306
>1k	0.0012989629003260332	0.37786193468479384
>5k	0.0	0.0
>10k+	1.4432921114733703E-4	0.7055239123581734
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22529	0.687885922505738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012915608558743272	0.0
2	0.0	0.0	0.0	0.0545020361166826	0.0
3	0.0	0.0	0.0	0.09624113044245577	0.0
4	0.0	0.0	0.0	0.14790356467742885	0.0
5	0.0	0.0	0.0	0.2319924204003106	0.0
6	0.0	0.0	0.0	0.4348560214979236	0.0
7	0.0	0.0	0.0	0.5379976898464691	0.0
8	0.0	0.0	0.0	0.7844323864838615	0.0
9	0.0	0.0	0.0	0.9334046185361272	0.0
10	0.0	0.0	0.0	1.1435962244897648	0.0
11	0.0	0.0	0.0	1.2794696478618865	0.0
12	0.0	0.0	0.0	1.4037098635250695	0.0
13	0.0	0.0	0.0	1.4716465752111305	0.0
14	0.0	0.0	0.0	1.5049279305989087	0.0
15	0.0	0.0	0.0	1.5381176859259866	0.0
16	0.0	0.0	0.0	1.5981157256846876	0.0
17	0.0	0.0	0.0	1.658174832150522	0.0
18	0.0	0.0	0.0	1.762201967752504	0.0
19	6.106670713353792E-5	0.0	0.0	1.80778826462769	0.0
20	6.106670713353792E-5	0.0	0.0	1.8597865657518975	0.0
21	6.106670713353792E-5	0.0	0.0	1.9164564699718207	0.0
22	6.106670713353792E-5	0.0	0.0	1.9785918444801955	0.0
23	6.106670713353792E-5	0.0	0.0	2.046864423055491	0.0
24	6.106670713353792E-5	0.0	0.0	2.10527472842872	0.0
25	6.106670713353792E-5	0.0	0.0	2.1579142299778296	0.0
26	6.106670713353792E-5	0.0	0.0	2.209973597809171	0.0
27	6.106670713353792E-5	0.0	0.0	2.266246568432726	0.0
28	6.106670713353792E-5	0.0	0.0	2.32621407483786	0.0
29	6.106670713353792E-5	0.0	0.0	2.387433448739232	0.0
30	6.106670713353792E-5	0.0	0.0	2.478056442125402	0.0
31	6.106670713353792E-5	0.0	0.0	2.5500235564822766	0.0
32	6.106670713353792E-5	0.0	0.0	2.624799739367294	0.0
33	6.106670713353792E-5	0.0	0.0	2.700125522616513	0.0
34	6.106670713353792E-5	0.0	0.0	2.777863440797507	0.0
35	6.106670713353792E-5	0.0	0.0	2.8619522965203883	0.0
36	6.106670713353792E-5	0.0	0.0	2.938255147083744	0.0
37	6.106670713353792E-5	0.0	0.0	3.016878532518174	0.0
38	6.106670713353792E-5	0.0	0.0	3.1162645983780073	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15965	0.0	42.23614	1
GCGATAC	210	0.0	38.761906	9
CACGACG	430	0.0	38.372093	26
CGGTCTA	465	0.0	37.849464	31
ACGGGTA	455	0.0	37.23077	5
GTTGATC	1980	0.0	35.88889	16
TTGATCC	2000	0.0	35.75	17
AGGGCGA	3455	0.0	35.658466	6
GGTACCT	2055	0.0	35.649635	8
GACCGAT	2150	0.0	35.506977	9
ATAACGC	155	0.0	35.48387	11
GGGCGAT	6475	0.0	35.471813	7
CGTTAGG	490	0.0	35.469387	2
TACGTCT	25	0.002353378	35.2	35
TTAGGGA	3065	0.0	35.171288	4
TAGCATA	2010	0.0	35.13433	30
CGGTAGT	1720	0.0	35.046513	12
GTTTTTT	20115	0.0	34.84564	2
TGCCAGT	2065	0.0	34.6247	24
CGACGGT	490	0.0	34.571426	28
>>END_MODULE