FastQCFastQC Report
Thu 26 May 2016
SRR1547183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547183_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2032945
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63390.31181364965604086No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34320.1688191269316189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT207.85791E-444.00000444
TACGTAC207.85791E-444.00000417
CGACGGT1500.044.028
TATAGCG1150.042.086961
CGGTCTA1600.041.25000431
CGTTTTT57650.040.794451
ACGGTCT1650.040.00000430
TCGTTGA557.8216544E-1140.024
ACGGGAT12900.039.3953485
ATAGCGG3850.037.7142872
CGCGCAC357.2929706E-637.71428734
TCGTCGG357.2929706E-637.7142872
TTCACGG2000.037.3999982
TAACGGG4900.037.2653053
GTTAACG651.0913936E-1137.2307661
AGGGCGA18800.036.9787256
TGGGCGA12800.036.953136
GACGGTC1850.036.86486429
TAGGGAC24350.036.8624235
TACCGCT301.3014476E-436.66666444
Produced by FastQC (version 0.11.5)