##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547183_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2032945
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.619836247414465	31.0	30.0	31.0	28.0	33.0
2	30.936198962588758	31.0	31.0	33.0	28.0	34.0
3	31.12480170393198	31.0	31.0	34.0	28.0	34.0
4	34.944376262023816	35.0	35.0	37.0	32.0	37.0
5	34.73822410345582	35.0	35.0	37.0	32.0	37.0
6	34.777477993747986	35.0	35.0	37.0	32.0	37.0
7	35.42354121729806	35.0	35.0	37.0	35.0	37.0
8	34.387653871600065	35.0	35.0	37.0	33.0	37.0
9	36.498198426420785	39.0	37.0	39.0	32.0	39.0
10	35.91757032285674	38.0	35.0	39.0	30.0	39.0
11	35.66531116188584	37.0	35.0	39.0	30.0	39.0
12	35.33177287137625	37.0	34.0	39.0	30.0	39.0
13	35.11569078356768	37.0	34.0	39.0	29.0	39.0
14	36.02464896984424	38.0	34.0	40.0	29.0	41.0
15	36.26397221764485	38.0	34.0	40.0	30.0	41.0
16	36.36481262405033	38.0	34.0	40.0	30.0	41.0
17	36.2938648118862	38.0	34.0	40.0	30.0	41.0
18	36.230413513400514	38.0	34.0	40.0	30.0	41.0
19	36.24838448654538	38.0	34.0	40.0	30.0	41.0
20	36.11829636315788	38.0	34.0	40.0	30.0	41.0
21	35.95456886438148	38.0	34.0	40.0	29.0	41.0
22	35.89515063122711	38.0	34.0	40.0	29.0	41.0
23	35.80441674516526	38.0	34.0	40.0	29.0	41.0
24	35.74668916276633	38.0	34.0	40.0	29.0	41.0
25	35.56935431111024	37.0	34.0	40.0	29.0	41.0
26	35.405921950667626	37.0	34.0	40.0	28.0	41.0
27	35.20733369569762	37.0	34.0	40.0	27.0	41.0
28	35.235401843138895	37.0	34.0	40.0	27.0	41.0
29	35.12490008337658	37.0	34.0	40.0	27.0	41.0
30	35.15552117740519	37.0	34.0	40.0	27.0	41.0
31	35.121477954396205	37.0	34.0	40.0	27.0	41.0
32	34.91086576370733	37.0	33.0	40.0	26.0	41.0
33	34.77719662853644	37.0	33.0	40.0	25.0	41.0
34	34.72286658025672	37.0	33.0	40.0	25.0	41.0
35	34.56558932976544	37.0	33.0	40.0	25.0	41.0
36	34.51649355983561	37.0	33.0	40.0	24.0	41.0
37	34.51915374001756	37.0	33.0	40.0	24.0	41.0
38	34.57351035074731	37.0	33.0	40.0	24.0	41.0
39	34.4868759361419	37.0	33.0	40.0	24.0	41.0
40	34.41237367464442	37.0	33.0	40.0	24.0	41.0
41	34.45981617800777	37.0	33.0	40.0	24.0	41.0
42	34.32788245623959	37.0	33.0	40.0	24.0	41.0
43	34.38321400726532	37.0	33.0	40.0	24.0	41.0
44	34.085945266596	36.0	33.0	40.0	23.0	41.0
45	34.01640182100352	36.0	33.0	40.0	23.0	41.0
46	33.9657019742295	36.0	32.0	40.0	23.0	41.0
47	33.92087341270915	36.0	32.0	40.0	23.0	41.0
48	33.92470824345961	36.0	32.0	40.0	23.0	41.0
49	33.944058004520535	36.0	32.0	40.0	24.0	41.0
50	33.357646173408526	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	5.0
13	8.0
14	35.0
15	75.0
16	200.0
17	542.0
18	1202.0
19	2328.0
20	4137.0
21	6386.0
22	9410.0
23	13487.0
24	19255.0
25	26390.0
26	34513.0
27	42766.0
28	50642.0
29	60693.0
30	74284.0
31	91197.0
32	111356.0
33	136856.0
34	185257.0
35	208206.0
36	188820.0
37	223584.0
38	270489.0
39	270815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.58861159549324	24.683943736795634	28.26731662686398	13.460128040847144
2	29.66012361377214	26.022346890840627	30.201702456288782	14.11582703909845
3	29.109985759575395	25.981027524109113	30.884160663470972	14.024826052844519
4	25.317359790845302	29.684816854366446	29.975921630934433	15.021901723853818
5	22.99275189441918	33.37389845765626	29.094687755940274	14.538661891984288
6	21.11891861314497	42.65196549832878	26.099279616516924	10.129836272009326
7	90.52581353651968	3.2831680148749722	5.088529202708386	1.1024892458969622
8	88.84347584415713	5.271072262161544	4.620784133363175	1.264667760318159
9	85.42341283212286	5.812847863567386	6.440951427608716	2.322787876701042
10	37.66363575994432	39.508102777005774	12.261374508410212	10.566886954639699
11	31.07536111404883	25.025320409553625	28.551977549810744	15.3473409265868
12	30.344992117347005	24.07674580473156	28.61843286463726	16.959829213284177
13	22.443696214113025	31.092380757964435	29.73853203111742	16.725390996805128
14	18.503058370983965	34.13815917302239	29.778867603402947	17.5799148525907
15	17.43559220736419	26.394663898924957	39.43451495244584	16.735228941265014
16	20.21550017339377	24.64355897478781	37.10877569240683	18.03216515941159
17	20.67055429438573	23.194380566124515	29.827467049034773	26.30759809045498
18	21.802803322273846	24.877210155710067	33.221115180194246	20.098871341821837
19	25.849494206680458	25.739751936230444	28.331410835020133	20.07934302206897
20	28.13460275610014	25.320261984460966	27.842022287863173	18.70311297157572
21	24.46716463062208	27.31564306953705	29.89416831247279	18.323023987368078
22	23.19118323417505	25.281205344955225	28.667081499991394	22.86052992087833
23	20.061683911763474	29.881378984674946	28.76452633986655	21.29241076369503
24	19.82040832388481	26.84883260491553	34.89258194392863	18.438177127271025
25	19.9489410682532	27.6611024892459	32.732169340537986	19.657787101962914
26	19.530336531485112	31.516297784740853	28.584885474029054	20.368480209744977
27	19.454485979699403	30.800685704728853	29.493026127121	20.251802188450746
28	18.109540592588587	28.93408331263266	34.17711743308353	18.779258661695224
29	19.713322298438964	25.918654956233446	33.5837910027079	20.784231742619696
30	20.218303987564838	28.530334071998997	31.205369550086203	20.04599239034996
31	24.710948894338017	27.645361778110082	28.222111272070816	19.42157805548109
32	25.648455811642716	27.055478628295404	28.73117570814754	18.56488985191434
33	25.22704746070356	27.42897618971492	27.81344305920721	19.530533290374308
34	20.808629844880212	27.67949944538588	30.1151285450418	21.39674216469211
35	20.77616462816259	27.31574144898165	30.94904190718391	20.959052015671844
36	25.45897700134534	26.99881206820647	28.206075422601202	19.33613550784699
37	21.38105064327859	30.79429104082993	29.097983467334338	18.726674848557142
38	21.018817528265643	31.21166583454053	26.98853141624589	20.780985220947933
39	20.798004864863533	30.653264106997486	28.3338703211351	20.21486070700388
40	22.905243378448507	27.10274995142515	28.479717847752887	21.512288822373453
41	19.21778503599458	26.97406963788986	29.125234573488218	24.682910752627347
42	20.728352218087554	28.05466945736358	27.70429106542479	23.51268725912408
43	21.206082801059548	27.579693498840353	28.378190260926882	22.83603343917322
44	20.574880284513352	28.97751783742305	29.92068157279218	20.526920305271418
45	19.877861919530535	31.925457894827453	27.0046164554378	21.19206373020421
46	21.06864671695496	30.5155328845591	28.38256814621153	20.033252252274412
47	20.86150879635209	28.160328980862737	29.07825838869227	21.899903834092903
48	21.515387774878317	26.9025969713888	30.37642434989633	21.20559090383655
49	20.84945731438873	26.404452653662542	31.43951262823146	21.306577403717267
50	19.99394966415717	30.27042049834108	29.150075383249423	20.58555445425233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	970.0
1	1554.0
2	2138.0
3	7369.0
4	12600.0
5	9658.0
6	6716.0
7	6981.5
8	7247.0
9	7486.5
10	7726.0
11	7730.5
12	7735.0
13	7777.5
14	7820.0
15	7752.0
16	7684.0
17	7769.5
18	7855.0
19	8608.0
20	9361.0
21	11142.5
22	12924.0
23	14424.0
24	15924.0
25	19089.5
26	22255.0
27	26319.0
28	30383.0
29	37413.0
30	44443.0
31	50321.0
32	56199.0
33	64375.0
34	72551.0
35	80057.0
36	87563.0
37	95137.5
38	102712.0
39	112670.0
40	122628.0
41	139030.0
42	155432.0
43	164723.5
44	174015.0
45	170733.0
46	167451.0
47	161225.0
48	154999.0
49	151170.0
50	147341.0
51	140150.0
52	132959.0
53	118248.5
54	103538.0
55	93930.0
56	84322.0
57	77652.0
58	70982.0
59	64197.5
60	57413.0
61	51838.0
62	46263.0
63	39223.5
64	32184.0
65	26733.0
66	21282.0
67	17423.0
68	13564.0
69	11389.0
70	9214.0
71	7899.5
72	6585.0
73	5265.0
74	3945.0
75	3286.5
76	2628.0
77	2217.5
78	1807.0
79	1403.5
80	1000.0
81	689.0
82	378.0
83	263.5
84	149.0
85	91.5
86	34.0
87	24.0
88	14.0
89	11.5
90	9.0
91	5.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2032945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.78319799089871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.17622355860227	17.02928155957793
2	7.549073203947966	3.288859124987732
3	2.86724834772051	1.8737351534241917
4	1.5583036705697015	1.3577934954385602
5	0.9138572300569898	0.995336648887284
6	0.6367274845726398	0.8321976517615631
7	0.45919709435768946	0.7001946856267263
8	0.33842690511499834	0.589761622365368
9	0.30379748029200543	0.5955912596103212
>10	4.585878798533634	25.484608141699944
>50	2.08770924599518	32.27355588259576
>100	0.522448688763724	14.396679967640852
>500	4.433165890715489E-4	0.05470607242852012
>1k	4.433165890715489E-4	0.22162477709347517
>5k	2.2165829453577444E-4	0.3060739568617732
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6339	0.31181364965604086	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3432	0.1688191269316189	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012395810019454535	0.0
2	0.0	0.0	0.0	0.053272469250274845	0.0
3	0.0	0.0	0.0	0.08450794291040829	0.0
4	0.0	0.0	0.0	0.13699337660389238	0.0
5	0.0	0.0	0.0	0.2332084734215633	0.0
6	0.0	0.0	0.0	0.41122607842317427	0.0
7	0.0	0.0	0.0	0.4982426971708531	0.0
8	0.0	0.0	0.0	0.7672612884263962	0.0
9	0.0	0.0	0.0	0.8901864044526536	0.0
10	0.0	0.0	0.0	1.062547191389831	0.0
11	0.0	0.0	0.0	1.1853739279714897	0.0
12	0.0	0.0	0.0	1.3044130559360927	0.0
13	0.0	0.0	0.0	1.3659493985326705	0.0
14	0.0	0.0	0.0	1.393790781354144	0.0
15	0.0	0.0	0.0	1.427879258907644	0.0
16	0.0	0.0	0.0	1.4918750876191929	0.0
17	0.0	0.0	0.0	1.5672829319042079	0.0
18	0.0	0.0	0.0	1.6898637198743693	0.0
19	0.0	0.0	0.0	1.7410702207880686	0.0
20	0.0	0.0	0.0	1.7966054172641168	0.0
21	0.0	0.0	0.0	1.8647823723711168	0.0
22	0.0	0.0	0.0	1.9381734380418556	0.0
23	0.0	0.0	0.0	2.0253868156787322	0.0
24	0.0	0.0	0.0	2.0930226838404384	0.0
25	0.0	0.0	0.0	2.1486562597610854	0.0
26	0.0	0.0	0.0	2.203306041235744	0.0
27	0.0	0.0	0.0	2.267400249391892	0.0
28	0.0	0.0	0.0	2.3300187658790574	0.0
29	0.0	0.0	0.0	2.399917361266537	0.0
30	0.0	0.0	0.0	2.4933286439131406	0.0
31	4.918972229942276E-5	0.0	0.0	2.5783284840465432	0.0
32	4.918972229942276E-5	0.0	0.0	2.657179608892518	0.0
33	4.918972229942276E-5	0.0	0.0	2.7368177692952833	0.0
34	4.918972229942276E-5	0.0	0.0	2.820538676648901	0.0
35	4.918972229942276E-5	0.0	0.0	2.9157699790205833	0.0
36	4.918972229942276E-5	0.0	0.0	3.0055904119393295	0.0
37	4.918972229942276E-5	0.0	0.0	3.09757519263925	0.0
38	9.837944459884552E-5	0.0	0.0	3.1947740839029093	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAT	20	7.85791E-4	44.000004	44
TACGTAC	20	7.85791E-4	44.000004	17
CGACGGT	150	0.0	44.0	28
TATAGCG	115	0.0	42.08696	1
CGGTCTA	160	0.0	41.250004	31
CGTTTTT	5765	0.0	40.79445	1
ACGGTCT	165	0.0	40.000004	30
TCGTTGA	55	7.8216544E-11	40.0	24
ACGGGAT	1290	0.0	39.395348	5
ATAGCGG	385	0.0	37.714287	2
CGCGCAC	35	7.2929706E-6	37.714287	34
TCGTCGG	35	7.2929706E-6	37.714287	2
TTCACGG	200	0.0	37.399998	2
TAACGGG	490	0.0	37.265305	3
GTTAACG	65	1.0913936E-11	37.230766	1
AGGGCGA	1880	0.0	36.978725	6
TGGGCGA	1280	0.0	36.95313	6
GACGGTC	185	0.0	36.864864	29
TAGGGAC	2435	0.0	36.862423	5
TACCGCT	30	1.3014476E-4	36.666664	44
>>END_MODULE