FastQCFastQC Report
Thu 26 May 2016
SRR1547182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547182_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences712970
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60260.8451968526024938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG207.8544684E-444.02
CGTTTTT56400.042.5957451
GCAACGG501.3460522E-939.6000022
TACGGGA2950.039.525424
AGGGCCG950.039.368426
GGCGCGA452.348861E-839.111118
GCGTAAG452.348861E-839.111111
ACGAAAG452.348861E-839.111111
CGTTAGG850.038.8235322
GTACCGG404.1216117E-738.52
GGGCCGA2250.038.1333357
TTAGACG357.2863204E-637.7142831
TATCGCG357.2863204E-637.7142831
TAGGGTC1650.037.3333325
ACGGGAA3450.036.9855085
ATAGGGA12200.036.9672134
GTTTTTT64700.036.6893352
ATTAGCG601.9826984E-1036.6666681
TCGATAG301.3005994E-436.6666681
GCGATGT1800.036.6666689
Produced by FastQC (version 0.11.5)