##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547182_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 712970 Sequences flagged as poor quality 0 Sequence length 50 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.659914161886196 31.0 30.0 31.0 28.0 34.0 2 30.998437521915367 31.0 31.0 33.0 28.0 34.0 3 31.115317615046916 31.0 31.0 34.0 28.0 34.0 4 34.954364138743564 35.0 35.0 37.0 32.0 37.0 5 34.77629213010365 35.0 35.0 37.0 32.0 37.0 6 34.82248902478365 35.0 35.0 37.0 32.0 37.0 7 35.483934807916185 35.0 35.0 37.0 35.0 37.0 8 34.45482278356733 37.0 35.0 37.0 33.0 37.0 9 36.57388669930011 39.0 37.0 39.0 32.0 39.0 10 35.99992566307138 38.0 35.0 39.0 30.0 39.0 11 35.7462277515183 37.0 35.0 39.0 30.0 39.0 12 35.586891454058375 37.0 35.0 39.0 30.0 39.0 13 35.51566966352021 37.0 35.0 39.0 30.0 39.0 14 36.469213290881804 38.0 35.0 40.0 30.0 41.0 15 36.603352174705805 38.0 35.0 40.0 30.0 41.0 16 36.628434576489894 38.0 35.0 40.0 31.0 41.0 17 36.54210275327153 38.0 35.0 40.0 30.0 41.0 18 36.39583432683002 38.0 35.0 40.0 30.0 41.0 19 36.33078390395108 38.0 35.0 40.0 30.0 41.0 20 36.14914933307152 38.0 34.0 40.0 30.0 41.0 21 36.01060633687252 38.0 34.0 40.0 30.0 41.0 22 35.9613237583629 38.0 34.0 40.0 30.0 41.0 23 35.84451519699286 38.0 34.0 40.0 29.0 41.0 24 35.75256883178815 37.0 34.0 40.0 29.0 41.0 25 35.57913656956113 37.0 34.0 40.0 29.0 41.0 26 35.41082794507483 37.0 34.0 40.0 27.0 41.0 27 35.220065360393846 37.0 34.0 40.0 27.0 41.0 28 35.22671641163022 37.0 34.0 40.0 27.0 41.0 29 35.102643870008556 37.0 33.0 40.0 27.0 41.0 30 35.06437858535422 37.0 33.0 40.0 27.0 41.0 31 34.94107325693928 37.0 33.0 40.0 26.0 41.0 32 34.59847819683858 37.0 33.0 40.0 24.0 41.0 33 34.3097535660687 37.0 33.0 40.0 23.0 41.0 34 34.078275383255956 37.0 33.0 40.0 23.0 41.0 35 33.798002721012104 37.0 33.0 40.0 21.0 41.0 36 33.751477621779316 37.0 33.0 40.0 20.0 41.0 37 33.73615439639816 37.0 33.0 40.0 19.0 41.0 38 33.754841017153595 37.0 33.0 40.0 19.0 41.0 39 33.66324389525506 37.0 32.0 40.0 18.0 41.0 40 33.617313491451256 37.0 32.0 40.0 18.0 41.0 41 33.63237022595621 37.0 32.0 40.0 18.0 41.0 42 33.48962649199826 37.0 32.0 40.0 18.0 41.0 43 33.54564567934135 37.0 32.0 40.0 18.0 41.0 44 33.28049567302972 37.0 32.0 40.0 18.0 41.0 45 33.21595158281555 37.0 31.0 40.0 18.0 41.0 46 33.15726608412696 36.0 31.0 40.0 18.0 41.0 47 33.116779107115306 36.0 31.0 40.0 18.0 41.0 48 33.11667110818127 36.0 31.0 40.0 18.0 41.0 49 33.10003646717253 36.0 31.0 40.0 18.0 41.0 50 32.56415697715192 36.0 31.0 39.0 17.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 17.0 15 38.0 16 94.0 17 254.0 18 574.0 19 1196.0 20 1986.0 21 3005.0 22 4406.0 23 6376.0 24 8888.0 25 12560.0 26 16866.0 27 19570.0 28 20447.0 29 21933.0 30 25121.0 31 29765.0 32 35772.0 33 43842.0 34 57099.0 35 65553.0 36 65495.0 37 77947.0 38 95547.0 39 98618.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.253446849096033 23.95037659368557 27.473245718613686 18.32293083860471 2 34.10311794325147 25.011571314361053 27.977193991332033 12.908116751055443 3 26.465068656465206 25.289142600670434 35.10414182924948 13.14164691361488 4 23.786414575648344 28.04283490188928 34.324165112136555 13.84658541032582 5 21.319971387295396 31.759821591371306 33.49046944471717 13.429737576616127 6 19.854972860008136 40.246854706369135 30.45065009747956 9.447522336143175 7 86.19675442164467 2.6688359959044563 10.123287094828674 1.0111224876222003 8 84.62053101813541 4.860793581777634 9.501942578229098 1.0167328218578622 9 81.18616491577487 5.440060591609745 11.172559855253377 2.2012146373620207 10 33.55905578074814 38.88943433805069 17.466373059174998 10.085136822026172 11 25.544272550037167 25.35197834410985 33.89623686831143 15.20751223754155 12 25.40162980209546 23.698753103216124 34.224301162741774 16.675315931946646 13 21.36176837735108 27.24476485686635 34.89487636225928 16.498590403523288 14 18.678485770789795 30.476738151675388 34.19358458280152 16.6511914947333 15 18.149010477299186 26.305594905816516 39.74416875885381 15.801225858030493 16 20.62246658344671 24.786456653155113 38.29936743481493 16.291709328583252 17 20.66496486528185 23.857946337153034 34.315328835715384 21.16175996184973 18 21.302719609520736 24.801885072303183 35.59111884090495 18.304276477271134 19 23.748544819557626 25.53459472348065 33.02607402836024 17.690786428601484 20 25.33570837482643 25.7961765572184 32.16446694811844 16.70364811983674 21 23.373634234259505 25.974725444268344 33.77337054855043 16.878269772921723 22 21.982131085459418 25.135559700969186 33.41907794156837 19.46323127200303 23 19.47431168211846 27.951386453847988 33.52581455040184 19.04848731363171 24 19.232366018205536 26.31429092388179 36.90800454437073 17.545338513541946 25 19.453693703802404 27.53762430396791 34.576910669453135 18.431771322776555 26 19.11384770747717 29.73070395668822 32.50347139430831 18.65197694152629 27 18.72883852055486 28.61508899392681 34.11966842924667 18.536404056271653 28 18.451547750957264 27.742682020281357 36.41415487327658 17.391615355484802 29 19.334614359650477 25.97640854453904 36.17557540990505 18.513401685905436 30 19.945860274625858 27.174074645497004 34.44380548971205 18.436259590165083 31 22.350449528030634 26.823288497412236 32.394069876712905 18.43219209784423 32 22.8988596995666 27.23803245578355 32.283125517202684 17.57998232744716 33 22.848647208157427 27.514060900178126 31.740185421546492 17.897106470117958 34 20.399876572646818 28.1985216769289 32.077787284177454 19.323814466246827 35 20.2324080957123 28.149852027434534 32.00681655609633 19.610923320756836 36 22.64849853430018 28.711306226068416 29.827762738965173 18.812432500666226 37 20.968483947431167 30.641261203136178 30.274345344123876 18.115909505308778 38 20.94057253460875 30.552477663856827 29.311471730928368 19.195478070606057 39 20.747016003478407 30.25639227456976 29.56197315455068 19.434618567401152 40 22.010603531705403 28.432893389623686 29.587359916967053 19.969143161703858 41 19.49408811029917 28.474129346255804 30.17335932788196 21.858423215563068 42 20.585017602423665 29.320869040773108 28.72575283672525 21.368360520077985 43 20.672679074855886 28.9274443524973 29.437844509586657 20.962032063060157 44 20.63508983547695 29.718501479725656 30.068165560963294 19.578243123834103 45 19.93506038122221 30.589786386523976 29.01524608328541 20.4599071489684 46 20.752345821002287 30.630461309732524 28.99070087100439 19.626491998260796 47 20.298890556404896 29.138533178114084 30.104772991850993 20.457803273630027 48 20.967502138939928 27.750676746567176 31.082794507482784 20.19902660701011 49 20.65528703872533 27.782936168422236 31.08321528255046 20.478561510301976 50 19.69718220963014 30.215296576293532 30.09649774885339 19.99102346522294 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 364.0 1 516.0 2 668.0 3 6101.5 4 11535.0 5 8970.0 6 6405.0 7 6421.5 8 6438.0 9 6486.5 10 6535.0 11 6585.0 12 6635.0 13 6434.5 14 6234.0 15 5920.5 16 5607.0 17 5331.5 18 5056.0 19 4980.0 20 4904.0 21 5390.0 22 5876.0 23 6515.0 24 7154.0 25 7903.5 26 8653.0 27 10115.5 28 11578.0 29 13935.5 30 16293.0 31 19057.0 32 21821.0 33 24007.0 34 26193.0 35 29208.0 36 32223.0 37 34101.0 38 35979.0 39 39113.5 40 42248.0 41 46241.5 42 50235.0 43 52767.5 44 55300.0 45 54593.5 46 53887.0 47 52652.0 48 51417.0 49 50174.5 50 48932.0 51 45950.0 52 42968.0 53 39179.0 54 35390.0 55 32257.0 56 29124.0 57 25690.0 58 22256.0 59 20430.5 60 18605.0 61 16638.0 62 14671.0 63 11655.5 64 8640.0 65 6957.5 66 5275.0 67 4213.5 68 3152.0 69 2461.5 70 1771.0 71 1532.0 72 1293.0 73 1019.0 74 745.0 75 566.5 76 388.0 77 318.0 78 248.0 79 201.5 80 155.0 81 119.0 82 83.0 83 55.0 84 27.0 85 15.0 86 3.0 87 3.0 88 3.0 89 2.0 90 1.0 91 1.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 712970.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.27273495768453 #Duplication Level Percentage of deduplicated Percentage of total 1 72.34423423307229 26.964674682859506 2 7.157286290551991 5.335432698480267 3 3.759390969345386 4.203683496081698 4 2.5211108150446395 3.758747808324434 5 2.0094299439715977 3.744847485884411 6 1.6824830293985193 3.762644641534389 7 1.388770436242721 3.6234290671 8 1.2285465257517079 3.663303123002205 9 1.0306169770893487 3.457252208694719 >10 6.854785980040626 39.92453240294657 >50 0.019956038605969305 0.5108472962927274 >100 0.0030122318979867455 0.20490027112565523 >500 0.0 0.0 >1k 0.0 0.0 >5k 3.765289872483398E-4 0.845704817673419 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6026 0.8451968526024938 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.011360926827215732 0.0 2 0.0 0.0 0.0 0.054139725374139164 0.0 3 0.0 0.0 0.0 0.09902239925943589 0.0 4 0.0 0.0 0.0 0.135629830147131 0.0 5 0.0 0.0 0.0 0.2099667587696537 0.0 6 0.0 0.0 0.0 0.38038066117788966 0.0 7 0.0 0.0 0.0 0.4684629086777845 0.0 8 0.0 0.0 0.0 0.6803932844299199 0.0 9 0.0 0.0 0.0 0.7928804858549449 0.0 10 0.0 0.0 0.0 0.9512321696565073 0.0 11 0.0 0.0 0.0 1.051236377407184 0.0 12 0.0 0.0 0.0 1.155308077478716 0.0 13 0.0 0.0 0.0 1.220107437900613 0.0 14 0.0 0.0 0.0 1.2612031361768377 0.0 15 0.0 0.0 0.0 1.2926210078965454 0.0 16 0.0 0.0 0.0 1.3487243502531663 0.0 17 0.0 0.0 0.0 1.4117003520484732 0.0 18 0.0 0.0 0.0 1.5115643014432585 0.0 19 0.0 0.0 0.0 1.5621975679201088 0.0 20 0.0 0.0 0.0 1.6128308343969593 0.0 21 0.0 0.0 0.0 1.6697757268889295 0.0 22 0.0 0.0 0.0 1.7345750873108265 0.0 23 0.0 0.0 0.0 1.806106848815518 0.0 24 0.0 0.0 0.0 1.865997166781211 0.0 25 0.0 0.0 0.0 1.9208381839348079 0.0 26 0.0 0.0 0.0 1.9760999761560796 0.0 27 0.0 0.0 0.0 2.0441252787634823 0.0 28 0.0 0.0 0.0 2.1047168885086327 0.0 29 0.0 0.0 0.0 2.178072008639915 0.0 30 0.0 0.0 0.0 2.262788055598412 0.0 31 0.0 0.0 0.0 2.3406314431182236 0.0 32 0.0 0.0 0.0 2.4128644964023733 0.0 33 0.0 0.0 0.0 2.487201425024896 0.0 34 0.0 0.0 0.0 2.569252563221454 0.0 35 0.0 0.0 0.0 2.6605607529068545 0.0 36 0.0 0.0 0.0 2.746819641780159 0.0 37 0.0 0.0 0.0 2.848927724869209 0.0 38 0.0 0.0 0.0 2.946687798925621 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACGG 20 7.8544684E-4 44.0 2 CGTTTTT 5640 0.0 42.595745 1 GCAACGG 50 1.3460522E-9 39.600002 2 TACGGGA 295 0.0 39.52542 4 AGGGCCG 95 0.0 39.36842 6 GGCGCGA 45 2.348861E-8 39.11111 8 GCGTAAG 45 2.348861E-8 39.11111 1 ACGAAAG 45 2.348861E-8 39.11111 1 CGTTAGG 85 0.0 38.823532 2 GTACCGG 40 4.1216117E-7 38.5 2 GGGCCGA 225 0.0 38.133335 7 TTAGACG 35 7.2863204E-6 37.714283 1 TATCGCG 35 7.2863204E-6 37.714283 1 TAGGGTC 165 0.0 37.333332 5 ACGGGAA 345 0.0 36.985508 5 ATAGGGA 1220 0.0 36.967213 4 GTTTTTT 6470 0.0 36.689335 2 ATTAGCG 60 1.9826984E-10 36.666668 1 TCGATAG 30 1.3005994E-4 36.666668 1 GCGATGT 180 0.0 36.666668 9 >>END_MODULE