FastQCFastQC Report
Thu 26 May 2016
SRR1547181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547181_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1536554
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62300.4054527208285553No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19040.12391364052288431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG408.3164196E-944.01
TTTAGCG1100.042.01
CGTTTTT46000.041.3217351
ACGGGTC1200.040.3333365
GGCGATA7000.039.2857138
CGGTCTA1350.039.1111131
GGGCGAT30900.038.161817
CGAAAGG2550.037.960782
CGACGGT1450.037.93103828
TGGGCGA9800.037.2653056
TAGGGCG8650.037.132955
GTTGATC8950.037.1173216
GCGGGAT6700.037.1044775
ATAGGGC11100.037.0630654
AGGGCGA17000.036.882356
GTAGCAT9050.036.7071829
GGCGATT8650.036.624288
TTCATCG2050.036.48780415
GGTACCT9250.036.389198
AGGGTAC7800.036.3846176
Produced by FastQC (version 0.11.5)