##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547181_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1536554
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.559071793116285	31.0	30.0	31.0	28.0	33.0
2	30.88050989421784	31.0	31.0	33.0	28.0	34.0
3	31.07241789094298	31.0	31.0	34.0	28.0	34.0
4	34.89995405303035	35.0	35.0	37.0	32.0	37.0
5	34.69466025925545	35.0	35.0	37.0	32.0	37.0
6	34.72037949854024	35.0	35.0	37.0	31.0	37.0
7	35.35626278022119	35.0	35.0	37.0	33.0	37.0
8	34.32069487958119	35.0	35.0	37.0	32.0	37.0
9	36.433971731549946	39.0	37.0	39.0	32.0	39.0
10	35.7468953255141	37.0	35.0	39.0	30.0	39.0
11	35.58421571907007	37.0	35.0	39.0	30.0	39.0
12	35.14755029761401	37.0	34.0	39.0	30.0	39.0
13	34.90339682171925	37.0	33.0	39.0	27.0	39.0
14	35.807224477629816	38.0	34.0	40.0	27.0	41.0
15	36.0376602449377	38.0	34.0	40.0	30.0	41.0
16	36.12594676138945	38.0	34.0	40.0	30.0	41.0
17	36.055652453477066	38.0	34.0	40.0	30.0	41.0
18	35.99509551893393	38.0	34.0	40.0	30.0	41.0
19	35.98597836457424	38.0	34.0	40.0	29.0	41.0
20	35.85845144394535	38.0	34.0	40.0	29.0	41.0
21	35.68650174351178	37.0	34.0	40.0	29.0	41.0
22	35.61960529860975	37.0	34.0	40.0	29.0	41.0
23	35.51023914551653	37.0	34.0	40.0	29.0	41.0
24	35.42118467688086	37.0	34.0	40.0	28.0	41.0
25	35.201400666686624	37.0	33.0	40.0	27.0	41.0
26	35.0194109676588	37.0	33.0	40.0	27.0	41.0
27	34.8160318478882	37.0	33.0	40.0	26.0	41.0
28	34.838577101748456	37.0	33.0	40.0	26.0	41.0
29	34.67623916894558	36.0	33.0	40.0	25.0	41.0
30	34.71496608645059	36.0	33.0	40.0	26.0	41.0
31	34.67573153953587	37.0	33.0	40.0	25.0	41.0
32	34.462634570604095	36.0	33.0	40.0	25.0	41.0
33	34.34539886004657	36.0	33.0	40.0	24.0	41.0
34	34.26154303721184	36.0	33.0	40.0	24.0	41.0
35	34.06630876623926	36.0	33.0	40.0	23.0	41.0
36	34.01587122873651	36.0	33.0	40.0	23.0	41.0
37	34.01018643015475	36.0	32.0	40.0	23.0	41.0
38	34.025069083156204	36.0	32.0	40.0	23.0	41.0
39	33.90538959255581	36.0	32.0	40.0	23.0	41.0
40	33.821084062128634	36.0	32.0	40.0	23.0	41.0
41	33.833197531619454	36.0	32.0	40.0	23.0	41.0
42	33.667854172388346	36.0	32.0	40.0	23.0	41.0
43	33.71445780623395	36.0	32.0	40.0	23.0	41.0
44	33.409855429747346	35.0	32.0	39.0	22.0	41.0
45	33.30271894121521	35.0	31.0	39.0	22.0	41.0
46	33.265928825150304	35.0	31.0	39.0	22.0	41.0
47	33.165704557080325	35.0	31.0	39.0	22.0	41.0
48	33.16787304578948	35.0	31.0	39.0	22.0	41.0
49	33.17732666733483	35.0	31.0	39.0	22.0	41.0
50	32.610320236060694	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	0.0
12	3.0
13	11.0
14	33.0
15	80.0
16	219.0
17	540.0
18	1227.0
19	2339.0
20	4030.0
21	6240.0
22	9024.0
23	13046.0
24	17766.0
25	23718.0
26	30607.0
27	37088.0
28	43757.0
29	51471.0
30	60552.0
31	72707.0
32	87327.0
33	106116.0
34	142325.0
35	158862.0
36	140964.0
37	162136.0
38	188331.0
39	176031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.370480959341485	23.70128221982436	27.55731331277651	14.37092350805764
2	30.187809865452174	25.294783001443488	30.5299390714547	13.987468061649638
3	29.31013163221078	26.146559118651215	30.33235408583102	14.210955163306984
4	25.620446791977376	29.27524838046694	30.03200668508884	15.072298142466845
5	22.932874471056664	32.92289109266579	29.470360299735642	14.673874136541897
6	20.789116425455923	42.798170451542866	26.230317971252553	10.182395151748654
7	89.38735638317951	3.599678241051079	5.659482191969823	1.353483183799593
8	87.61078361059879	5.575072532432963	5.196693380122013	1.6174504768462419
9	85.05207106291091	4.562416940764854	7.331730612786794	3.0537813835374483
10	40.88167418782548	27.25566429816329	16.31215043532476	15.550511078686464
11	36.13468840014734	25.45924191404923	22.02005266329722	16.386017022506206
12	32.693221325121016	22.342202096379303	26.192050523444017	18.772526055055664
13	23.707790289179552	29.475827077994005	27.554124358792464	19.262258274033975
14	20.52065856455419	32.37679899307151	28.32819412789918	18.774348314475116
15	20.168376770357565	23.971171855984235	36.91630752970608	18.944143843952116
16	21.89926289606483	21.45456651702446	36.153301478503195	20.492869108407515
17	22.142599609255516	21.436539164910574	28.803803836376723	27.617057389457187
18	24.216005425126614	23.341646307256365	31.149897758230434	21.292450509386587
19	28.19432314126285	25.198723897760832	25.738242847306374	20.868710113669938
20	29.93106652938979	24.714198134266677	24.812404900836547	20.542330435506987
21	26.11128538274607	26.31765626199925	27.200085385869937	20.37097296938474
22	25.412188572611182	23.72588272198699	26.297481246998156	24.564447458403674
23	23.12857211656733	27.732185136350562	25.673682799302856	23.46555994777925
24	23.12375614524449	25.041814345607115	32.07488965568408	19.75953985346431
25	22.838247142632152	25.18342993477613	30.043916452008844	21.934406470582875
26	21.640502058502335	28.900318504914242	26.649893202581882	22.80928623400154
27	21.588567665047893	28.36886956136914	27.764530241045872	22.278032532537093
28	20.538035109732558	27.26763914577685	31.581252595092657	20.61307314939794
29	21.487822751429498	25.330642463590607	31.516367143621377	21.665167641358522
30	22.779153872886994	26.289085837529953	29.54826188991731	21.383498399665747
31	27.322046605586266	25.636391561897597	26.017048538482868	21.024513294033273
32	28.015351233995027	25.381014920399803	26.049263481791073	20.554370363814094
33	26.83719543862435	25.792064580873824	25.65070931447902	21.7200306660228
34	22.404289078027848	26.86661191210982	28.075681036917675	22.653417972944652
35	22.207745383501003	26.07236712800201	29.086969934021194	22.632917554475796
36	27.155049545932002	26.891733059820872	25.068887914124723	20.884329480122403
37	22.990796288317885	29.55229689291753	27.03452010147382	20.42238671729077
38	23.443822996132905	29.389204674876378	24.75897365143041	22.407998677560307
39	23.304875715399522	28.103014928209486	25.664506421512034	22.92760293487896
40	24.8158541775948	24.992548260588304	26.58259976544918	23.608997796367717
41	21.648506983809227	24.86355832596837	27.411662720607282	26.076271969615128
42	23.23042340197611	26.220751109300423	25.256059988780088	25.292765499943382
43	22.537834661196417	26.37850671047031	26.834071565333854	24.249587062999414
44	22.254538402164844	27.080987716669895	27.96185490389534	22.702618977269918
45	21.056728237341478	29.728470330362615	26.2133319102355	23.001469522060404
46	23.549839445929006	28.172325866842296	26.13458427103766	22.143250416191034
47	22.482255748903064	27.56661985195444	27.17314197873944	22.77798242040306
48	23.056397627418235	25.827533558859628	28.49766425390842	22.618404559813712
49	22.623350692523662	25.558685213796583	29.264705308111527	22.553258785568225
50	21.93388582503446	28.386441348628168	27.540587574533664	22.13908525180371
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1035.0
1	1496.5
2	1958.0
3	6704.0
4	11450.0
5	8465.0
6	5480.0
7	5603.5
8	5727.0
9	5948.5
10	6170.0
11	6194.0
12	6218.0
13	6278.5
14	6339.0
15	6056.0
16	5773.0
17	5595.5
18	5418.0
19	5584.0
20	5750.0
21	6180.0
22	6610.0
23	7078.0
24	7546.0
25	8346.0
26	9146.0
27	11322.5
28	13499.0
29	16524.0
30	19549.0
31	22395.0
32	25241.0
33	28860.0
34	32479.0
35	37451.0
36	42423.0
37	47309.0
38	52195.0
39	59686.5
40	67178.0
41	78511.0
42	89844.0
43	101066.0
44	112288.0
45	115211.5
46	118135.0
47	118675.5
48	119216.0
49	120021.5
50	120827.0
51	113777.5
52	106728.0
53	100263.0
54	93798.0
55	87121.0
56	80444.0
57	79390.5
58	78337.0
59	75253.5
60	72170.0
61	66668.5
62	61167.0
63	54163.5
64	47160.0
65	38664.0
66	30168.0
67	26649.0
68	23130.0
69	20159.0
70	17188.0
71	14148.0
72	11108.0
73	9589.5
74	8071.0
75	6347.5
76	4624.0
77	3534.5
78	2445.0
79	1928.5
80	1412.0
81	1054.0
82	696.0
83	493.0
84	290.0
85	184.0
86	78.0
87	52.0
88	26.0
89	17.0
90	8.0
91	8.0
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1536554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.792052726905116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.44931948590181	19.449196650843025
2	7.009404659936204	3.4755505982670547
3	2.6588779848903057	1.9775712958742304
4	1.35052776511357	1.339294222473798
5	0.8490213326662955	1.0524490822865022
6	0.633201755697399	0.9419022788411285
7	0.5142673177348926	0.8924819719905266
8	0.4222164979852419	0.8374090944175476
9	0.36409894524360725	0.8124084223461043
>10	6.0187825530844465	38.18807780574645
>50	1.625231043577344	26.83810240310653
>100	0.10272194900832807	3.421991686170293
>500	0.0018112182360043544	0.25178212239612385
>1k	2.5874546228633633E-4	0.12213838497890951
>5k	2.5874546228633633E-4	0.39964398026187303
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6230	0.4054527208285553	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1904	0.12391364052288431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01835275558164568	0.0
2	0.0	0.0	0.0	0.0707427138909534	0.0
3	0.0	0.0	0.0	0.11467218203850955	0.0
4	0.0	0.0	0.0	0.1799481176711004	0.0
5	0.0	0.0	0.0	0.28863287590283193	0.0
6	0.0	0.0	0.0	0.5048309398823602	0.0
7	0.0	0.0	0.0	0.607137790146002	0.0
8	0.0	0.0	0.0	0.9155551968886222	0.0
9	0.0	0.0	0.0	1.0707726510099873	0.0
10	0.0	0.0	0.0	1.2982296749740003	0.0
11	0.0	0.0	0.0	1.460801247466734	0.0
12	0.0	0.0	0.0	1.6073629693456917	0.0
13	0.0	0.0	0.0	1.6818152827691055	0.0
14	0.0	0.0	0.0	1.721124021674474	0.0
15	0.0	0.0	0.0	1.761408970983122	0.0
16	0.0	0.0	0.0	1.8367724141162627	0.0
17	0.0	0.0	0.0	1.9195550563143242	0.0
18	0.0	0.0	0.0	2.0458116018050783	0.0
19	0.0	0.0	0.0	2.1036683383727484	0.0
20	0.0	0.0	0.0	2.165364835859983	0.0
21	0.0	0.0	0.0	2.2374742443155267	0.0
22	0.0	0.0	0.0	2.3151805924165374	0.0
23	0.0	0.0	0.0	2.405577675760175	0.0
24	0.0	0.0	0.0	2.4771013579737517	0.0
25	0.0	0.0	0.0	2.5385375326867785	0.0
26	0.0	0.0	0.0	2.601861047512811	0.0
27	0.0	0.0	0.0	2.669219565339064	0.0
28	0.0	0.0	0.0	2.732087515310233	0.0
29	0.0	0.0	0.0	2.8055636183303676	0.0
30	0.0	0.0	0.0	2.9078704685940098	0.0
31	0.0	0.0	0.0	2.9882451251306494	0.0
32	0.0	0.0	0.0	3.075062770328931	0.0
33	0.0	0.0	0.0	3.156804121430161	0.0
34	0.0	0.0	0.0	3.2389359566927034	0.0
35	0.0	0.0	0.0	3.338249095052956	0.0
36	0.0	0.0	0.0	3.42246351250916	0.0
37	0.0	0.0	0.0	3.5060271230298445	0.0
38	0.0	0.0	0.0	3.605145019309442	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	40	8.3164196E-9	44.0	1
TTTAGCG	110	0.0	42.0	1
CGTTTTT	4600	0.0	41.321735	1
ACGGGTC	120	0.0	40.333336	5
GGCGATA	700	0.0	39.285713	8
CGGTCTA	135	0.0	39.11111	31
GGGCGAT	3090	0.0	38.16181	7
CGAAAGG	255	0.0	37.96078	2
CGACGGT	145	0.0	37.931038	28
TGGGCGA	980	0.0	37.265305	6
TAGGGCG	865	0.0	37.13295	5
GTTGATC	895	0.0	37.11732	16
GCGGGAT	670	0.0	37.104477	5
ATAGGGC	1110	0.0	37.063065	4
AGGGCGA	1700	0.0	36.88235	6
GTAGCAT	905	0.0	36.70718	29
GGCGATT	865	0.0	36.62428	8
TTCATCG	205	0.0	36.487804	15
GGTACCT	925	0.0	36.38919	8
AGGGTAC	780	0.0	36.384617	6
>>END_MODULE