FastQCFastQC Report
Thu 26 May 2016
SRR1547180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547180_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences839628
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34700.4132782613252535No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA11590.13803732128990456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT750.044.028
CGTGCCG207.8552694E-444.02
GCGTTAG454.802132E-1044.01
TAGCCGT254.4418117E-544.044
GTATGCG207.8552694E-444.01
ACTAACG207.8552694E-444.029
CCGAAAC207.8552694E-444.018
TACGGGT750.041.0666664
CGTTTTT23600.040.7372861
CACGTAG603.6379788E-1240.3333321
TCGTCCC1600.039.87538
ATAGGGC4050.039.111114
CGGTCTA850.038.82353231
TAGTACG404.122776E-738.51
GACCGAT6300.038.063499
AGGGCGA8950.037.8547486
CTCGTCC1750.037.71428737
TAACGCG357.287861E-637.7142871
GCGGGAT4900.037.7142875
TTAGGGA8750.037.211434
Produced by FastQC (version 0.11.5)