##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547180_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	839628
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.644493751994933	31.0	30.0	31.0	28.0	33.0
2	30.96929949930207	31.0	31.0	33.0	28.0	34.0
3	31.143974474410097	31.0	31.0	34.0	28.0	34.0
4	34.97053933408605	35.0	35.0	37.0	32.0	37.0
5	34.762173248152756	35.0	35.0	37.0	32.0	37.0
6	34.79329893714836	35.0	35.0	37.0	32.0	37.0
7	35.426351908226025	35.0	35.0	37.0	35.0	37.0
8	34.37790902637835	36.0	35.0	37.0	33.0	37.0
9	36.48787558299628	39.0	37.0	39.0	32.0	39.0
10	35.94633814022401	38.0	35.0	39.0	30.0	39.0
11	35.706177021252266	37.0	35.0	39.0	30.0	39.0
12	35.293314420195614	37.0	34.0	39.0	30.0	39.0
13	35.12128228215352	37.0	34.0	39.0	29.0	39.0
14	36.02216219563902	38.0	34.0	40.0	29.0	41.0
15	36.22766153582301	38.0	34.0	40.0	30.0	41.0
16	36.32424954860962	38.0	34.0	40.0	30.0	41.0
17	36.2318741156798	38.0	34.0	40.0	30.0	41.0
18	36.19112749932113	38.0	34.0	40.0	30.0	41.0
19	36.16886406837314	38.0	34.0	40.0	30.0	41.0
20	36.04216510168789	38.0	34.0	40.0	30.0	41.0
21	35.87038188340551	38.0	34.0	40.0	29.0	41.0
22	35.808654547013674	38.0	34.0	40.0	29.0	41.0
23	35.702977985488815	37.0	34.0	40.0	29.0	41.0
24	35.61011066805776	37.0	34.0	40.0	29.0	41.0
25	35.41537442772275	37.0	34.0	40.0	28.0	41.0
26	35.246568718527726	37.0	34.0	40.0	27.0	41.0
27	35.05643570724178	37.0	33.0	40.0	27.0	41.0
28	35.05557580261735	37.0	33.0	40.0	27.0	41.0
29	34.91880570919503	37.0	33.0	40.0	27.0	41.0
30	34.96076953126861	37.0	33.0	40.0	27.0	41.0
31	34.93541901889885	37.0	33.0	40.0	27.0	41.0
32	34.72618826432658	37.0	33.0	40.0	25.0	41.0
33	34.6025728060522	37.0	33.0	40.0	25.0	41.0
34	34.48886888002782	37.0	33.0	40.0	25.0	41.0
35	34.34136546184739	37.0	33.0	40.0	24.0	41.0
36	34.28017407709128	37.0	33.0	40.0	24.0	41.0
37	34.2668681844817	37.0	33.0	40.0	24.0	41.0
38	34.29032261906463	37.0	33.0	40.0	24.0	41.0
39	34.193161733529614	36.0	33.0	40.0	23.0	41.0
40	34.103337430385835	36.0	33.0	40.0	23.0	41.0
41	34.136383017240966	36.0	33.0	40.0	23.0	41.0
42	33.997865721486185	36.0	33.0	40.0	23.0	41.0
43	34.04042980939178	36.0	33.0	40.0	23.0	41.0
44	33.729738646162346	36.0	32.0	40.0	23.0	41.0
45	33.64937924890547	36.0	32.0	40.0	23.0	41.0
46	33.6047130395842	35.0	32.0	39.0	23.0	41.0
47	33.549324224537536	35.0	32.0	39.0	23.0	41.0
48	33.538239553707115	35.0	32.0	39.0	23.0	41.0
49	33.54478888269567	35.0	32.0	39.0	23.0	41.0
50	32.941028646019426	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	5.0
14	16.0
15	38.0
16	104.0
17	263.0
18	582.0
19	1109.0
20	1947.0
21	2996.0
22	4408.0
23	6248.0
24	8660.0
25	11696.0
26	15325.0
27	18516.0
28	22045.0
29	26265.0
30	31367.0
31	38495.0
32	46519.0
33	56749.0
34	76972.0
35	87973.0
36	78478.0
37	90962.0
38	106108.0
39	105778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.53184029117657	23.047587741237784	28.06695346034196	14.353618507243684
2	30.87045691663451	25.817623995388434	29.31083765667653	14.001081431300527
3	29.8367848618674	26.14610279790574	30.562701577365214	13.454410762861649
4	26.24043028579323	29.336325134464307	28.84420243250582	15.579042147236633
5	22.745191918325737	33.49983564149838	28.86040008194105	14.894572358234836
6	21.392807290847852	41.90998870928554	25.901708852015414	10.795495147851192
7	88.8007546199031	3.8384856150580973	5.8231740723272685	1.5375856927115341
8	86.49759179065015	6.005159427746574	5.575802617349588	1.9214461642536933
9	82.95221217015154	5.972764128876121	7.72687428242031	3.3481494185520253
10	40.63370921408052	32.71651255079631	13.922951592848262	12.726826642274913
11	35.0250348964065	25.21997837137399	23.65488049469527	16.100106237524237
12	31.753824312671803	23.201465410872434	27.042333033200418	18.002377243255346
13	23.32104217582072	30.84187282939588	27.793499025759026	18.043585969024377
14	19.847003673055212	33.47577736807253	28.716645943203417	17.960573015668842
15	19.295688090440052	25.03203799777997	38.07007388986551	17.602200021914467
16	21.545493956847555	22.671945194776736	36.31489183304987	19.467669015325832
17	21.040627515995176	22.44636910631851	29.391111301671693	27.121892076014614
18	22.964455687518758	23.975379572858458	31.838385570752763	21.22177916887002
19	27.569709442753222	25.626706112707055	26.49268485567418	20.310899588865546
20	29.79748174191428	24.46571576936453	25.661483418847393	20.0753190698738
21	25.170075319069873	27.141186334900695	27.414878970210616	20.273859375818816
22	24.95664746768807	24.53241197292134	26.820806357101002	23.690134202289588
23	22.872033805447174	28.3005092731543	26.350002620207995	22.477454301190527
24	21.611594658586895	25.486882285964736	32.73675961259034	20.16476344285803
25	21.70842325410777	26.733029389205697	30.415969929540225	21.142577427146307
26	20.657481646633986	29.88668791417151	27.544221964965438	21.911608474229062
27	21.115660744996596	29.394207911122542	28.15282482242136	21.337306521459503
28	20.376762089877896	28.170689876945502	31.89936495686185	19.55318307631475
29	20.58899893762476	26.12740404083713	31.987737426574625	21.295859594963485
30	22.251044510187846	27.308045944156223	29.92384722758174	20.517062318074196
31	26.339045386766518	26.590823555193488	25.9669758512103	21.10315520682969
32	27.016726455049138	26.51674312910003	27.044953241197295	19.421577174653535
33	26.046773094751487	26.642870414040505	26.57343490212332	20.736921589084687
34	21.892671516433467	27.904857865626205	28.629583577489075	21.572887040451246
35	21.935428546927927	26.5990414802746	29.480436574292423	21.98509339850505
36	25.889441514575502	27.75764981634724	26.237810077796357	20.1150985912809
37	22.866674289089932	30.0670058645019	27.003268113974283	20.063051732433888
38	22.56022905381907	30.260067553726174	25.83810925790945	21.3415941345453
39	22.221984021495235	29.047387652627116	26.776143720790635	21.954484605087014
40	23.79196501307722	26.11930521611952	27.542792760603508	22.545937010199754
41	20.013744181947242	26.74922703864092	27.953450813931884	25.283577965479953
42	22.6475296202604	27.28994268890509	25.5964546203795	24.46607307045501
43	22.619898335929722	26.926329279156956	26.40788539686623	24.045886988047087
44	22.082993897297374	27.617111387423954	28.511197816175738	21.788696899102934
45	20.75716865087872	30.422520449532414	26.5612866650469	22.259024234541965
46	21.858847013201085	30.117504418623486	26.679315125269763	21.344333442905665
47	22.202451561882167	27.751456597445532	27.116770760384362	22.929321080287938
48	22.564754867631855	26.175996989142813	28.849204647772588	22.410043495452747
49	22.099310647096097	26.303315277718227	29.230206710590878	22.3671673645948
50	20.88698804708752	29.580719080354633	27.349850171742723	22.182442700815123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	628.0
1	872.0
2	1116.0
3	3684.0
4	6252.0
5	4638.0
6	3024.0
7	3122.0
8	3220.0
9	3322.0
10	3424.0
11	3455.0
12	3486.0
13	3509.5
14	3533.0
15	3462.0
16	3391.0
17	3429.0
18	3467.0
19	3471.0
20	3475.0
21	3671.0
22	3867.0
23	4545.5
24	5224.0
25	6043.5
26	6863.0
27	8307.0
28	9751.0
29	11305.5
30	12860.0
31	15191.5
32	17523.0
33	19902.0
34	22281.0
35	24726.0
36	27171.0
37	29497.0
38	31823.0
39	36477.5
40	41132.0
41	48071.0
42	55010.0
43	59907.0
44	64804.0
45	68580.5
46	72357.0
47	68608.5
48	64860.0
49	63182.0
50	61504.0
51	57175.0
52	52846.0
53	49245.0
54	45644.0
55	42674.5
56	39705.0
57	38305.0
58	36905.0
59	35285.0
60	33665.0
61	30166.0
62	26667.0
63	24174.0
64	21681.0
65	18651.0
66	15621.0
67	13658.0
68	11695.0
69	9872.5
70	8050.0
71	7246.0
72	6442.0
73	5007.5
74	3573.0
75	2934.5
76	2296.0
77	1823.5
78	1351.0
79	1102.0
80	853.0
81	552.0
82	251.0
83	243.5
84	236.0
85	139.0
86	42.0
87	36.5
88	31.0
89	21.5
90	12.0
91	13.0
92	14.0
93	8.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	839628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.990083633748554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57412536399912	21.433161731194815
2	6.639812347850207	3.716978058574473
3	2.510100654444655	2.1077378174109858
4	1.4016987990677583	1.5693466646092586
5	1.0303882612867084	1.4420326804323862
6	0.8498138279522442	1.4271816070499592
7	0.6773370634554386	1.3271104738048762
8	0.648607259130247	1.4523657142809625
9	0.5963728658946096	1.5023273753959585
>10	8.689329614101847	56.455041034414656
>50	0.35935169851185084	6.082034304989822
>100	0.022223617239669313	0.9413943585794187
>500	0.0	0.0
>1k	8.386270656478986E-4	0.5432881792624522
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3470	0.4132782613252535	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1159	0.13803732128990456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02119986470198707	0.0
2	0.0	0.0	0.0	0.07932084208721005	0.0
3	0.0	0.0	0.0	0.12493628130553054	0.0
4	0.0	0.0	0.0	0.20580542811816663	0.0
5	0.0	0.0	0.0	0.32895520397128253	0.0
6	0.0	0.0	0.0	0.6064590509130234	0.0
7	0.0	0.0	0.0	0.7430671678409962	0.0
8	0.0	0.0	0.0	1.1595611389805962	0.0
9	0.0	0.0	0.0	1.3840653241673693	0.0
10	0.0	0.0	0.0	1.6797915267237395	0.0
11	0.0	0.0	0.0	1.860466778144607	0.0
12	0.0	0.0	0.0	2.0308993983049635	0.0
13	0.0	0.0	0.0	2.120701072379673	0.0
14	0.0	0.0	0.0	2.1667929130519705	0.0
15	0.0	0.0	0.0	2.207644337730519	0.0
16	0.0	0.0	0.0	2.2862505776367628	0.0
17	0.0	0.0	0.0	2.3718837389891716	0.0
18	0.0	0.0	0.0	2.507896354099673	0.0
19	0.0	0.0	0.0	2.5654218296674243	0.0
20	0.0	0.0	0.0	2.6365247466735267	0.0
21	0.0	0.0	0.0	2.7088186673145724	0.0
22	0.0	0.0	0.0	2.783256394498516	0.0
23	0.0	0.0	0.0	2.871747964574788	0.0
24	0.0	0.0	0.0	2.9451137884872827	0.0
25	0.0	0.0	0.0	3.0064504756868518	0.0
26	0.0	0.0	0.0	3.065166954889546	0.0
27	0.0	0.0	0.0	3.1317440580828655	0.0
28	0.0	0.0	0.0	3.1998694660016103	0.0
29	0.0	0.0	0.0	3.277403802636406	0.0
30	0.0	0.0	0.0	3.3907873486829883	0.0
31	0.0	0.0	0.0	3.474991305673465	0.0
32	0.0	0.0	0.0	3.565864883019623	0.0
33	0.0	0.0	0.0	3.6457812269243046	0.0
34	0.0	0.0	0.0	3.727603176644895	0.0
35	0.0	0.0	0.0	3.8213351627149166	0.0
36	0.0	0.0	0.0	3.9106604353356484	0.0
37	0.0	0.0	0.0	4.004511521769165	0.0
38	0.0	0.0	0.0	4.104913128194867	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	75	0.0	44.0	28
CGTGCCG	20	7.8552694E-4	44.0	2
GCGTTAG	45	4.802132E-10	44.0	1
TAGCCGT	25	4.4418117E-5	44.0	44
GTATGCG	20	7.8552694E-4	44.0	1
ACTAACG	20	7.8552694E-4	44.0	29
CCGAAAC	20	7.8552694E-4	44.0	18
TACGGGT	75	0.0	41.066666	4
CGTTTTT	2360	0.0	40.737286	1
CACGTAG	60	3.6379788E-12	40.333332	1
TCGTCCC	160	0.0	39.875	38
ATAGGGC	405	0.0	39.11111	4
CGGTCTA	85	0.0	38.823532	31
TAGTACG	40	4.122776E-7	38.5	1
GACCGAT	630	0.0	38.06349	9
AGGGCGA	895	0.0	37.854748	6
CTCGTCC	175	0.0	37.714287	37
TAACGCG	35	7.287861E-6	37.714287	1
GCGGGAT	490	0.0	37.714287	5
TTAGGGA	875	0.0	37.21143	4
>>END_MODULE