FastQCFastQC Report
Thu 26 May 2016
SRR1547179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547179_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1627520
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181251.1136575894612664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT148100.042.5887951
TACGGGA4700.037.914894
GTTTTTT167950.037.5290262
GTACGAC301.3013334E-436.66666435
TGGGCGA9750.036.1025666
TATTGCG800.035.751
TAGGGAC16050.035.5015565
GGGCGAT32950.035.3869487
AGGGATT23950.035.3653456
GACCGAT12200.035.344269
AGGGCGA16450.035.306996
TTAGGGA18250.035.24
TATTACG250.002353031435.21
CGTAATC250.002353031435.216
TGTAGCG1000.035.21
ATACGCG751.8189894E-1235.21
TTAGCGG3700.035.081082
TAGGGAT21050.034.8028535
GGACCGA12950.034.6563728
TAAGGGA21300.034.4976544
Produced by FastQC (version 0.11.5)