##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547179_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1627520
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.619409899724733	31.0	30.0	31.0	28.0	34.0
2	30.948897094966576	31.0	31.0	33.0	28.0	34.0
3	31.059030303775067	31.0	31.0	34.0	28.0	34.0
4	34.90926317341722	35.0	35.0	37.0	32.0	37.0
5	34.73216058788832	35.0	35.0	37.0	32.0	37.0
6	34.77422888812426	35.0	35.0	37.0	32.0	37.0
7	35.41564650511207	35.0	35.0	37.0	33.0	37.0
8	34.37438864038537	36.0	35.0	37.0	32.0	37.0
9	36.479651248525364	39.0	37.0	39.0	32.0	39.0
10	35.921239063114434	38.0	35.0	39.0	30.0	39.0
11	35.70776211659457	37.0	35.0	39.0	30.0	39.0
12	35.51984245969327	37.0	35.0	39.0	30.0	39.0
13	35.47912713822257	37.0	35.0	39.0	30.0	39.0
14	36.396502039913486	38.0	35.0	40.0	30.0	41.0
15	36.493107918796696	38.0	35.0	40.0	30.0	41.0
16	36.510556552300436	38.0	35.0	40.0	30.0	41.0
17	36.40607427251278	38.0	35.0	40.0	30.0	41.0
18	36.25320549056233	38.0	35.0	40.0	30.0	41.0
19	36.15905549547778	38.0	34.0	40.0	30.0	41.0
20	35.96007668108533	38.0	34.0	40.0	30.0	41.0
21	35.80701681085332	38.0	34.0	40.0	29.0	41.0
22	35.75237600766811	37.0	34.0	40.0	29.0	41.0
23	35.620434157491154	37.0	34.0	40.0	29.0	41.0
24	35.50174867282737	37.0	34.0	40.0	28.0	41.0
25	35.297719843688554	37.0	34.0	40.0	27.0	41.0
26	35.118350004915456	37.0	33.0	40.0	27.0	41.0
27	34.91652637141172	37.0	33.0	40.0	26.0	41.0
28	34.89556073043649	37.0	33.0	40.0	26.0	41.0
29	34.71014181085332	36.0	33.0	40.0	25.0	41.0
30	34.667435730436495	37.0	33.0	40.0	25.0	41.0
31	34.55414004128981	37.0	33.0	40.0	25.0	41.0
32	34.19295492528509	37.0	33.0	40.0	23.0	41.0
33	33.879151715493514	37.0	32.0	40.0	22.0	41.0
34	33.60353359712938	37.0	32.0	40.0	21.0	41.0
35	33.30633171942588	36.0	31.0	40.0	18.0	41.0
36	33.2182492381046	37.0	31.0	40.0	18.0	41.0
37	33.21418845851357	37.0	31.0	40.0	18.0	41.0
38	33.20081657982698	36.0	31.0	40.0	18.0	41.0
39	33.085812155918205	36.0	31.0	40.0	18.0	41.0
40	33.001324100471884	36.0	31.0	40.0	18.0	41.0
41	32.98811013075108	36.0	31.0	40.0	18.0	41.0
42	32.81610610007865	36.0	31.0	40.0	16.0	41.0
43	32.845716181675186	36.0	31.0	40.0	17.0	41.0
44	32.533357500983094	35.0	31.0	40.0	15.0	41.0
45	32.45830957530476	35.0	30.0	39.0	15.0	41.0
46	32.40013947601258	35.0	30.0	39.0	15.0	41.0
47	32.33803762780181	35.0	30.0	39.0	15.0	41.0
48	32.312551612268976	35.0	30.0	39.0	15.0	41.0
49	32.31176882618954	35.0	30.0	39.0	15.0	41.0
50	31.75516675678333	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	10.0
13	8.0
14	30.0
15	105.0
16	285.0
17	798.0
18	1702.0
19	3244.0
20	5595.0
21	8290.0
22	11670.0
23	16516.0
24	23268.0
25	31961.0
26	42437.0
27	49789.0
28	52561.0
29	54830.0
30	61768.0
31	73000.0
32	85136.0
33	102247.0
34	132777.0
35	148265.0
36	150607.0
37	175013.0
38	204046.0
39	191558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.19210823830122	22.539876622099882	25.619347227683836	19.64866791191506
2	36.32403902870625	23.966279984270546	26.7189957727094	12.990685214313801
3	27.61778657097916	24.871460872984663	34.52031311443177	12.990439441604403
4	24.315215788438852	27.568079040503342	33.93604994101455	14.180655230043257
5	21.451656508061344	31.082321569012976	33.752580613448686	13.713441309476995
6	19.688052988596148	39.9778190129768	30.881586708611874	9.45254128981518
7	83.78287209988203	3.142019760125836	11.710885273299253	1.3642228666928824
8	81.78940965395202	5.501191997640582	11.161276051907196	1.5481222965001966
9	78.60444111285882	5.179967066456941	13.263861580810065	2.951730239874164
10	35.2610720605584	32.6341918993315	19.585197109712936	12.51953893039717
11	27.95271333071176	25.60834889893826	30.480239874164372	15.958697896185608
12	25.888837003539127	22.379755701926857	33.58183002359418	18.149577270939833
13	22.938950058985448	25.186418600078646	33.98286964215493	17.891761698780968
14	20.356739087691704	27.296131537554068	34.14723014156508	18.199899233189146
15	19.70673171451042	23.94514353126229	38.405303775068816	17.942820979158476
16	22.583931380259536	22.807031557215886	36.36330121903264	18.24573584349194
17	22.326300137632717	23.01913340542666	33.22773299252851	21.426833464412113
18	23.257840149429807	23.180974734565474	34.553062327959104	19.008122788045615
19	25.5427275855289	24.499299547778215	31.069848604011014	18.88812426268187
20	26.725385863153754	24.12523348407393	30.80207923712151	18.347301415650808
21	25.295603126228865	24.792199174203695	31.752359418010222	18.159838281557217
22	24.027723161620134	23.184722768383796	32.01515188753441	20.77240218246166
23	22.002863252064493	25.889021333071177	31.914323633503738	20.1937917813606
24	21.616815768777034	24.473984958710187	34.57727093983484	19.33192833267794
25	22.236162996460873	25.308506193472276	32.64709496657491	19.80823584349194
26	21.29215001966182	26.08477929610696	32.03899184034605	20.584078843885177
27	21.012767892253244	25.129092115611485	32.86872050727487	20.9894194848604
28	20.445647365316553	25.86481272119544	33.97561934722768	19.713920566260324
29	21.430950157294536	24.6279001179709	33.82158130161227	20.119568423122296
30	21.931773495871017	24.821077467558002	33.41900560361778	19.828143432953205
31	23.699678037750687	25.066727290601655	31.17755849390484	20.056036177742822
32	24.28560017695635	25.300764353126233	31.09516319307904	19.31847227683838
33	23.988829630357845	25.555261993708218	30.07932314195832	20.376585233975618
34	22.075427644514352	26.87389402280771	30.700145005898545	20.350533326779395
35	21.798441801022413	26.810853322847034	30.751880161226897	20.638824714903656
36	23.747419386551314	28.179868757373182	28.041928824223355	20.030783031852142
37	22.386514451435314	28.879890876917024	28.190989972473457	20.542604699174206
38	22.792838183248133	29.22495576091231	27.567648938261897	20.414557117577665
39	22.67271677152969	27.96684526150216	27.4111531655525	21.94928480141565
40	23.533412799842708	26.608275167125445	28.839768482107747	21.018543550924104
41	21.275806134486828	26.66977978765238	28.95417567833268	23.100238399528116
42	22.650781557215886	27.40918698387731	27.629583661030278	22.310447797876524
43	22.395300825796305	26.973800629178136	28.36899085725521	22.26190768777035
44	22.01214117184428	27.352044828942194	29.26618413291388	21.369629866299647
45	21.546954876130556	27.732378096736138	28.850582481321275	21.870084545812034
46	22.30049400314589	27.964080318521432	28.630247247345654	21.105178430987024
47	22.049682953204876	27.401015041289817	29.13524872198191	21.414053283523398
48	22.12452074321667	26.660133208808496	29.76307510813999	21.452270939834843
49	22.397021234762093	26.545173023987417	29.576164962642547	21.481640778607943
50	21.274392941407786	27.68869199764058	29.355030967361383	21.68188409359025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	990.0
1	1525.5
2	2061.0
3	17363.0
4	32665.0
5	24136.5
6	15608.0
7	15817.0
8	16026.0
9	16143.5
10	16261.0
11	16170.5
12	16080.0
13	15537.0
14	14994.0
15	14315.0
16	13636.0
17	12974.0
18	12312.0
19	11888.5
20	11465.0
21	11356.5
22	11248.0
23	11771.5
24	12295.0
25	13634.5
26	14974.0
27	17226.0
28	19478.0
29	22811.5
30	26145.0
31	29488.5
32	32832.0
33	37581.0
34	42330.0
35	47224.0
36	52118.0
37	57097.0
38	62076.0
39	67281.0
40	72486.0
41	80757.0
42	89028.0
43	94585.5
44	100143.0
45	103344.0
46	106545.0
47	108255.0
48	109965.0
49	111235.5
50	112506.0
51	106895.5
52	101285.0
53	95762.5
54	90240.0
55	85376.0
56	80512.0
57	77786.5
58	75061.0
59	70668.0
60	66275.0
61	61223.5
62	56172.0
63	49740.5
64	43309.0
65	37548.0
66	31787.0
67	26810.5
68	21834.0
69	19076.0
70	16318.0
71	13733.5
72	11149.0
73	9308.0
74	7467.0
75	6088.0
76	4709.0
77	3656.0
78	2603.0
79	2033.0
80	1463.0
81	1034.0
82	605.0
83	450.5
84	296.0
85	199.0
86	102.0
87	70.5
88	39.0
89	27.0
90	15.0
91	10.5
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1627520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.36914665833323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.71266832234336	23.436726498389962
2	5.894901728413128	3.6983607371010687
3	2.8575140150117986	2.6891332864544317
4	1.9676717162312993	2.4689673056765544
5	1.517201867604627	2.379666394759331
6	1.261619989617282	2.3745565488833615
7	1.0986688893506318	2.4125013863291636
8	0.9671735187063003	2.4271526361883304
9	0.8960681639840226	2.529800428669037
>10	8.698427376042561	51.14253201862511
>50	0.10784004199429213	2.1188237376377557
>100	0.01925200479731081	1.0206832687335927
>500	7.938927226752938E-4	0.17264515847581385
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.9847318066882344E-4	1.128450594076497
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18125	1.1136575894612664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013087396775462053	0.0
2	0.0	0.0	0.0	0.05308690523004326	0.0
3	0.0	0.0	0.0	0.10181134486826583	0.0
4	0.0	0.0	0.0	0.14826238694455368	0.0
5	0.0	0.0	0.0	0.24319209594966576	0.0
6	0.0	0.0	0.0	0.5009462249311837	0.0
7	0.0	0.0	0.0	0.6504989186000787	0.0
8	0.0	0.0	0.0	0.9997419386551317	0.0
9	0.0	0.0	0.0	1.2100619347227684	0.0
10	0.0	0.0	0.0	1.4728544042469525	0.0
11	0.0	0.0	0.0	1.6136207235548565	0.0
12	0.0	0.0	0.0	1.7558616791191506	0.0
13	0.0	0.0	0.0	1.8427423318914669	0.0
14	0.0	0.0	0.0	1.8957677939441604	0.0
15	0.0	0.0	0.0	1.9354600865119937	0.0
16	0.0	0.0	0.0	1.994814195831695	0.0
17	0.0	0.0	0.0	2.060558395595753	0.0
18	0.0	0.0	0.0	2.160280672434133	0.0
19	0.0	0.0	0.0	2.211892941407786	0.0
20	0.0	0.0	0.0	2.2730289028706254	0.0
21	0.0	0.0	0.0	2.335393727880456	0.0
22	0.0	0.0	0.0	2.402551120723555	0.0
23	0.0	0.0	0.0	2.481382717263075	0.0
24	0.0	0.0	0.0	2.5491545418796697	0.0
25	0.0	0.0	0.0	2.607279787652379	0.0
26	0.0	0.0	0.0	2.667739874164373	0.0
27	0.0	0.0	0.0	2.734528607943374	0.0
28	0.0	0.0	0.0	2.801133012190326	0.0
29	0.0	0.0	0.0	2.867430200550531	0.0
30	0.0	0.0	0.0	2.962912898151789	0.0
31	0.0	0.0	0.0	3.040945733385765	0.0
32	0.0	0.0	0.0	3.1506218049547776	0.0
33	0.0	0.0	0.0	3.240328843885175	0.0
34	0.0	0.0	0.0	3.3259806331104995	0.0
35	0.0	0.0	0.0	3.4228150806134487	0.0
36	0.0	0.0	0.0	3.5117848014156507	0.0
37	0.0	0.0	0.0	3.6018604994101455	0.0
38	0.0	0.0	0.0	3.7128268777035	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14810	0.0	42.588795	1
TACGGGA	470	0.0	37.91489	4
GTTTTTT	16795	0.0	37.529026	2
GTACGAC	30	1.3013334E-4	36.666664	35
TGGGCGA	975	0.0	36.102566	6
TATTGCG	80	0.0	35.75	1
TAGGGAC	1605	0.0	35.501556	5
GGGCGAT	3295	0.0	35.386948	7
AGGGATT	2395	0.0	35.365345	6
GACCGAT	1220	0.0	35.34426	9
AGGGCGA	1645	0.0	35.30699	6
TTAGGGA	1825	0.0	35.2	4
TATTACG	25	0.0023530314	35.2	1
CGTAATC	25	0.0023530314	35.2	16
TGTAGCG	100	0.0	35.2	1
ATACGCG	75	1.8189894E-12	35.2	1
TTAGCGG	370	0.0	35.08108	2
TAGGGAT	2105	0.0	34.802853	5
GGACCGA	1295	0.0	34.656372	8
TAAGGGA	2130	0.0	34.497654	4
>>END_MODULE