FastQCFastQC Report
Thu 26 May 2016
SRR1547178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547178_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2047827
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80880.39495523791804676No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37600.18360925996190108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTA207.8579265E-444.014
ATCACGC351.4468242E-744.012
GCTCGAT254.4440625E-544.027
CGTTTTT65350.041.710791
TAATGCG750.041.066671
AAATGCG1600.039.8751
TACGGGA5450.039.1559644
CGGTCTA1600.038.531
TAAGGGA21800.037.743124
AAGCGTA357.2929943E-637.71428718
GCGTATG1000.037.41
CGTAAGG4000.036.8500022
CGCGTAA601.9826984E-1036.66666431
GGGCGAT43700.036.4988567
AATGCGG3650.036.1643832
TCGACAC553.426976E-936.038
GACCGAT13100.035.6030549
AGGGCGA22900.035.5458536
TTAGGGA23550.035.4989364
ATTACGA250.002353172735.243
Produced by FastQC (version 0.11.5)