##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547178_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2047827
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.599406590498123	31.0	30.0	31.0	28.0	33.0
2	30.91743394339463	31.0	31.0	33.0	28.0	34.0
3	31.089178919898995	31.0	31.0	34.0	28.0	34.0
4	34.90616248345197	35.0	35.0	37.0	32.0	37.0
5	34.69642992303549	35.0	35.0	37.0	32.0	37.0
6	34.73885196356919	35.0	35.0	37.0	32.0	37.0
7	35.40486964963349	35.0	35.0	37.0	33.0	37.0
8	34.38710008218467	35.0	35.0	37.0	33.0	37.0
9	36.496512156544476	39.0	37.0	39.0	32.0	39.0
10	35.86775884876994	38.0	35.0	39.0	30.0	39.0
11	35.63334207430608	37.0	35.0	39.0	30.0	39.0
12	35.10905413396737	37.0	34.0	39.0	30.0	39.0
13	34.79727193752207	37.0	33.0	39.0	27.0	39.0
14	35.6771157915195	38.0	33.0	40.0	27.0	41.0
15	35.967363454041774	38.0	34.0	40.0	30.0	41.0
16	36.102819720611166	38.0	34.0	40.0	30.0	41.0
17	36.028262641326634	38.0	34.0	40.0	30.0	41.0
18	35.979056824624344	38.0	34.0	40.0	30.0	41.0
19	35.950133482955344	37.0	34.0	40.0	30.0	41.0
20	35.82109133242212	37.0	34.0	40.0	29.0	41.0
21	35.643117314109055	37.0	34.0	40.0	29.0	41.0
22	35.58177472999428	37.0	34.0	40.0	29.0	41.0
23	35.464648136781086	37.0	34.0	40.0	29.0	41.0
24	35.39593530117534	37.0	34.0	40.0	29.0	41.0
25	35.19078222916291	37.0	33.0	40.0	27.0	41.0
26	35.005102481801444	37.0	33.0	40.0	27.0	41.0
27	34.78835712196392	36.0	33.0	40.0	26.0	41.0
28	34.823868422479045	36.0	33.0	40.0	26.0	41.0
29	34.70865703011045	36.0	33.0	40.0	26.0	41.0
30	34.72929646889117	36.0	33.0	40.0	26.0	41.0
31	34.661644758077706	36.0	33.0	40.0	25.0	41.0
32	34.450769523011466	36.0	33.0	40.0	25.0	41.0
33	34.30439973689184	36.0	33.0	40.0	24.0	41.0
34	34.23061860205965	36.0	33.0	40.0	24.0	41.0
35	34.053916663858814	36.0	33.0	40.0	23.0	41.0
36	33.9484419338157	36.0	33.0	40.0	23.0	41.0
37	33.93293964773392	36.0	32.0	40.0	23.0	41.0
38	33.9455408098438	36.0	32.0	40.0	23.0	41.0
39	33.865985261450305	36.0	32.0	40.0	23.0	41.0
40	33.783346933114956	36.0	32.0	40.0	23.0	41.0
41	33.81052940507182	36.0	32.0	40.0	23.0	41.0
42	33.64511650642364	36.0	32.0	40.0	23.0	41.0
43	33.68178659623103	35.0	32.0	40.0	23.0	41.0
44	33.31634410523936	35.0	32.0	39.0	22.0	41.0
45	33.28429891782851	35.0	31.0	39.0	22.0	41.0
46	33.25624381356433	35.0	31.0	39.0	22.0	41.0
47	33.203650503680244	35.0	31.0	39.0	22.0	41.0
48	33.18923424683823	35.0	31.0	39.0	22.0	41.0
49	33.21653440451757	35.0	31.0	39.0	22.0	41.0
50	32.628021800669686	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	3.0
13	15.0
14	32.0
15	102.0
16	294.0
17	744.0
18	1688.0
19	3260.0
20	5174.0
21	8448.0
22	12242.0
23	17203.0
24	23850.0
25	32253.0
26	41045.0
27	49320.0
28	57237.0
29	67800.0
30	80586.0
31	96689.0
32	117122.0
33	142289.0
34	195437.0
35	218076.0
36	182632.0
37	210709.0
38	246027.0
39	237548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.68409538501055	24.24443080396928	28.268257035384337	13.803216775635832
2	30.729744260623576	26.197085984314107	29.377188600404235	13.695981154658085
3	29.575447535363093	26.02636843834953	30.478355837675743	13.919828188611636
4	25.588636149440358	28.65730357105361	30.413897267689116	15.340163011816916
5	22.294217236123952	33.64927799076777	29.90174462979539	14.154760143312886
6	20.49553013999718	42.06473496052156	26.763198258446636	10.67653664103462
7	88.7802534100781	3.90081779369058	5.952016454514957	1.3669123417163656
8	87.2563453846443	5.6306025850816495	5.503882896357944	1.6091691339160976
9	84.30546135000661	5.323008242395476	7.486667574946517	2.884862832651391
10	43.39121419924633	29.051038002721906	14.792899986180474	12.764847811851293
11	37.07803442380631	24.973935786567907	22.715786050286475	15.23224373933931
12	33.28215713534395	22.819798742764892	26.5715805094864	17.32646361240476
13	23.090866562458643	32.416752001023525	28.029613829683854	16.462767606833975
14	19.76172791939944	34.304069630881905	28.865866110760336	17.06833633895832
15	18.664320765377155	24.909965539081185	39.79432832949268	16.63138536604899
16	21.115797379368473	21.217075465847458	38.463307691518864	19.203819463265205
17	20.836721070676383	21.70129605674698	29.274592043175524	28.187390829401117
18	23.39831440839485	23.69247988233381	32.28124250730164	20.6279632019697
19	27.999142505690177	25.236750956013378	26.07407754658963	20.690028991706818
20	28.96543506848967	24.99967038231257	25.94227930386698	20.092615245330784
21	25.405905869978273	26.944170576909084	28.303367423127053	19.34655612998559
22	25.38495683473262	24.202288572228024	26.721446684705302	23.691307908334053
23	21.549671920528443	28.76859226878052	26.470009429507474	23.211726381183567
24	21.57311140052358	25.462551279966522	33.330110404834	19.6342269146759
25	21.163750648858525	25.575695603193044	32.299408104297875	20.96114564365056
26	20.338485624029765	30.285907940465673	27.92496631795557	21.450640117548993
27	20.900886647163066	29.324938092915076	28.60114648356526	21.1730287763566
28	20.095545180330173	27.13764395136894	33.19635887211175	19.57045199618913
29	20.565506754232658	25.76003734690479	32.624679721480376	21.04977617738217
30	22.009329889683062	28.021214682685596	29.862532333053526	20.106923094577812
31	27.020641880393214	26.779215236443317	26.236835435805855	19.963307447357614
32	28.21561586989526	26.214177271810556	26.764272567946414	18.80593429034777
33	26.636478569722932	26.22081845780918	26.346170843533166	20.796532128934718
34	21.839051833968398	27.339369976077084	28.626783414809942	22.19479477514458
35	22.249193901633294	25.965669951612124	29.726241523331804	22.058894623422777
36	27.588707444525344	25.90482496812475	26.538765237493205	19.967702349856705
37	22.709242528787833	29.541606786120116	27.82515319897628	19.92399748611577
38	22.870291289254414	31.061705896054697	24.917046215329712	21.150956599361177
39	22.615826434557214	29.435396642392153	26.09439176258541	21.854385160465213
40	24.41295089868431	25.553769922947595	26.748402086699706	23.28487709166839
41	21.0539757508813	24.602322364145017	27.444066320055356	26.899635564918327
42	22.635701160303093	26.61811764372674	25.93417315036866	24.81200804560151
43	22.200703477393354	26.51908584074729	27.446361435804878	23.833849246054477
44	21.886321451958587	27.97594718694499	28.41753722360336	21.72019413749306
45	20.666540679461693	30.625926897145124	25.98154043285883	22.725991990534354
46	22.841187268260455	29.704852997836245	26.726476406454257	20.727483327449047
47	22.623248936555676	27.286777642837993	27.155663051615196	22.93431036899113
48	23.27960320866948	26.372930916527615	29.198023075191408	21.14944279961149
49	22.108068699162576	25.22346858401613	30.18228590598718	22.48617681083412
50	21.792319370728094	29.31858990041639	27.165917824113073	21.72317290474244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1337.0
1	1907.5
2	2478.0
3	8654.0
4	14830.0
5	11196.5
6	7563.0
7	7717.0
8	7871.0
9	8330.0
10	8789.0
11	8989.5
12	9190.0
13	9174.5
14	9159.0
15	9446.5
16	9734.0
17	8985.0
18	8236.0
19	8616.5
20	8997.0
21	9204.5
22	9412.0
23	10557.0
24	11702.0
25	13430.0
26	15158.0
27	17589.5
28	20021.0
29	25533.0
30	31045.0
31	34552.0
32	38059.0
33	43630.5
34	49202.0
35	55858.5
36	62515.0
37	66903.0
38	71291.0
39	83456.0
40	95621.0
41	116136.5
42	136652.0
43	154544.0
44	172436.0
45	175929.5
46	179423.0
47	174014.5
48	168606.0
49	161698.0
50	154790.0
51	145605.0
52	136420.0
53	126547.5
54	116675.0
55	107248.0
56	97821.0
57	92399.5
58	86978.0
59	81793.5
60	76609.0
61	70886.0
62	65163.0
63	57636.0
64	50109.0
65	42285.0
66	34461.0
67	30435.0
68	26409.0
69	22764.5
70	19120.0
71	16459.5
72	13799.0
73	11238.0
74	8677.0
75	6893.0
76	5109.0
77	4235.0
78	3361.0
79	2480.5
80	1600.0
81	1246.0
82	892.0
83	596.5
84	301.0
85	212.5
86	124.0
87	82.5
88	41.0
89	34.0
90	27.0
91	20.0
92	13.0
93	7.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2047827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.550073649184178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.37140521883167	18.69202438542727
2	7.153525559640052	3.3693210756168934
3	2.7634691098561963	1.9523970319307662
4	1.5227885226833295	1.4344712744529915
5	0.9809669261308819	1.1550921678898043
6	0.6385282873248327	0.9022432916152348
7	0.4956831962560816	0.8171363044945615
8	0.36917805924708497	0.6955336387945386
9	0.3252059985659647	0.6892762695646458
>10	4.119342104758945	23.810633230203845
>50	1.7121259896288852	28.802251652189785
>100	0.5463448975860239	16.94082506795882
>500	8.206454229097165E-4	0.11764860865940993
>1k	4.1032271145485826E-4	0.23036902098072545
>5k	2.0516135572742913E-4	0.39077698022066015
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8088	0.39495523791804676	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3760	0.18360925996190108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018263261496210374	0.0
2	4.883224998986731E-5	0.0	0.0	0.07818043223377756	0.0
3	4.883224998986731E-5	0.0	0.0	0.12969845597308757	0.0
4	4.883224998986731E-5	0.0	0.0	0.21452007420548708	0.0
5	4.883224998986731E-5	0.0	0.0	0.34543933642832136	0.0
6	4.883224998986731E-5	0.0	0.0	0.6161164981221559	0.0
7	4.883224998986731E-5	0.0	0.0	0.7479147408448077	0.0
8	4.883224998986731E-5	0.0	0.0	1.116988886268225	0.0
9	4.883224998986731E-5	0.0	0.0	1.3047488874792645	0.0
10	4.883224998986731E-5	0.0	0.0	1.5748888944232105	0.0
11	4.883224998986731E-5	0.0	0.0	1.7497083493869354	0.0
12	4.883224998986731E-5	0.0	0.0	1.9102199551036294	0.0
13	4.883224998986731E-5	0.0	0.0	1.988742213087336	0.0
14	4.883224998986731E-5	0.0	0.0	2.023608439580101	0.0
15	4.883224998986731E-5	0.0	0.0	2.0650670198214987	0.0
16	4.883224998986731E-5	0.0	0.0	2.1501327993038473	0.0
17	4.883224998986731E-5	0.0	0.0	2.240570126285082	0.0
18	4.883224998986731E-5	0.0	0.0	2.3841369412552917	0.0
19	4.883224998986731E-5	0.0	0.0	2.444102944242849	0.0
20	4.883224998986731E-5	0.0	0.0	2.5181326352274875	0.0
21	4.883224998986731E-5	0.0	0.0	2.6039797307096744	0.0
22	4.883224998986731E-5	0.0	0.0	2.700911746939561	0.0
23	4.883224998986731E-5	0.0	0.0	2.805656923167826	0.0
24	4.883224998986731E-5	0.0	0.0	2.884911664901381	0.0
25	4.883224998986731E-5	0.0	0.0	2.959478510635908	0.0
26	4.883224998986731E-5	0.0	0.0	3.0301876086212363	0.0
27	4.883224998986731E-5	0.0	0.0	3.1043149641058547	0.0
28	4.883224998986731E-5	0.0	0.0	3.1790283065903515	0.0
29	4.883224998986731E-5	0.0	0.0	3.2717607493211096	0.0
30	4.883224998986731E-5	0.0	0.0	3.3887628202968316	0.0
31	4.883224998986731E-5	0.0	0.0	3.4884782747761407	0.0
32	4.883224998986731E-5	0.0	0.0	3.5849708007561185	0.0
33	9.766449997973462E-5	0.0	0.0	3.6803401849863295	0.0
34	9.766449997973462E-5	0.0	0.0	3.79060340546345	0.0
35	9.766449997973462E-5	0.0	0.0	3.910584243688554	0.0
36	9.766449997973462E-5	0.0	0.0	4.0163548971666065	0.0
37	9.766449997973462E-5	0.0	0.0	4.125885633893879	0.0
38	9.766449997973462E-5	0.0	0.0	4.24679428486879	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCTA	20	7.8579265E-4	44.0	14
ATCACGC	35	1.4468242E-7	44.0	12
GCTCGAT	25	4.4440625E-5	44.0	27
CGTTTTT	6535	0.0	41.71079	1
TAATGCG	75	0.0	41.06667	1
AAATGCG	160	0.0	39.875	1
TACGGGA	545	0.0	39.155964	4
CGGTCTA	160	0.0	38.5	31
TAAGGGA	2180	0.0	37.74312	4
AAGCGTA	35	7.2929943E-6	37.714287	18
GCGTATG	100	0.0	37.4	1
CGTAAGG	400	0.0	36.850002	2
CGCGTAA	60	1.9826984E-10	36.666664	31
GGGCGAT	4370	0.0	36.498856	7
AATGCGG	365	0.0	36.164383	2
TCGACAC	55	3.426976E-9	36.0	38
GACCGAT	1310	0.0	35.603054	9
AGGGCGA	2290	0.0	35.545853	6
TTAGGGA	2355	0.0	35.498936	4
ATTACGA	25	0.0023531727	35.2	43
>>END_MODULE