FastQCFastQC Report
Thu 26 May 2016
SRR1547177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547177_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences273091
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.6206722301357424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG408.272764E-944.00000420
TTAGACG207.8459264E-444.0000041
CGTTGAT408.272764E-944.00000425
TAAGTAG207.8459264E-444.0000041
ATTGCGT207.8459264E-444.00000411
CTATACG254.4338994E-544.01
ACGGGTA254.4338994E-544.05
CGTTTTT12300.041.6747971
TTACGGG750.041.0666663
GCCCTAC1700.040.11764526
AAGGGTG1100.040.05
GTCTCAA1600.039.87500442
ACTAATC1850.039.2432410
TCGTTGA452.339766E-839.1111124
TATGGCC452.339766E-839.1111110
ACGGGAT900.039.111115
ATCGTTG452.339766E-839.1111123
GTTACGG452.339766E-839.111112
TAATCGT452.339766E-839.1111121
TACGGGA800.038.5000044
Produced by FastQC (version 0.11.5)