##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547177_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	273091
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.569150942359872	31.0	30.0	31.0	28.0	33.0
2	30.906525663606637	31.0	31.0	33.0	28.0	34.0
3	31.079046911102893	31.0	31.0	34.0	28.0	34.0
4	34.922231051188064	35.0	35.0	37.0	32.0	37.0
5	34.73404103394107	35.0	35.0	37.0	32.0	37.0
6	34.762262396051135	35.0	35.0	37.0	32.0	37.0
7	35.33293297838449	35.0	35.0	37.0	33.0	37.0
8	34.277325140704015	35.0	35.0	37.0	32.0	37.0
9	36.39572523444566	39.0	37.0	39.0	32.0	39.0
10	35.81904200431358	37.0	35.0	39.0	30.0	39.0
11	35.621291071474346	37.0	35.0	39.0	30.0	39.0
12	35.23583713853624	37.0	34.0	39.0	30.0	39.0
13	35.08912413810781	37.0	34.0	39.0	29.0	39.0
14	35.99303528860343	38.0	34.0	40.0	29.0	41.0
15	36.16750460469221	38.0	34.0	40.0	30.0	41.0
16	36.229220296531196	38.0	34.0	40.0	30.0	41.0
17	36.14517138975653	38.0	34.0	40.0	30.0	41.0
18	36.0720968468386	38.0	34.0	40.0	30.0	41.0
19	36.030876154834836	38.0	34.0	40.0	30.0	41.0
20	35.867304305158356	38.0	34.0	40.0	29.0	41.0
21	35.71841254380408	38.0	34.0	40.0	29.0	41.0
22	35.69528106015943	37.0	34.0	40.0	29.0	41.0
23	35.55626146595824	37.0	34.0	40.0	29.0	41.0
24	35.45818793003065	37.0	34.0	40.0	28.0	41.0
25	35.25131915735048	37.0	33.0	40.0	27.0	41.0
26	35.070401441277816	37.0	33.0	40.0	27.0	41.0
27	34.852389862719754	37.0	33.0	40.0	26.0	41.0
28	34.8415692937519	37.0	33.0	40.0	26.0	41.0
29	34.64290657692857	36.0	33.0	40.0	25.0	41.0
30	34.68732766733433	37.0	33.0	40.0	25.0	41.0
31	34.64849811967439	37.0	33.0	40.0	25.0	41.0
32	34.41170159397417	37.0	33.0	40.0	24.0	41.0
33	34.23371696613949	36.0	33.0	40.0	24.0	41.0
34	34.05915976725707	36.0	33.0	40.0	23.0	41.0
35	33.85240084806896	36.0	32.0	40.0	23.0	41.0
36	33.79510492839383	36.0	32.0	40.0	23.0	41.0
37	33.80809327293832	36.0	32.0	40.0	23.0	41.0
38	33.785049672087325	36.0	32.0	40.0	23.0	41.0
39	33.66710363944619	36.0	32.0	40.0	22.0	41.0
40	33.562504806090274	36.0	32.0	40.0	22.0	41.0
41	33.56588463186264	36.0	32.0	40.0	22.0	41.0
42	33.40548022454054	36.0	32.0	40.0	21.0	41.0
43	33.42910604889945	35.0	32.0	40.0	22.0	41.0
44	33.126060543921255	35.0	31.0	39.0	21.0	41.0
45	33.046361835432144	35.0	31.0	39.0	20.0	41.0
46	32.98445206909052	35.0	31.0	39.0	20.0	41.0
47	32.90707493106694	35.0	31.0	39.0	20.0	41.0
48	32.88201734952818	35.0	31.0	39.0	20.0	41.0
49	32.86853832605249	35.0	31.0	39.0	20.0	40.0
50	32.303126064205706	35.0	30.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	3.0
14	9.0
15	22.0
16	43.0
17	106.0
18	237.0
19	464.0
20	762.0
21	1169.0
22	1753.0
23	2389.0
24	3365.0
25	4434.0
26	5714.0
27	7011.0
28	8191.0
29	9136.0
30	10716.0
31	12599.0
32	15447.0
33	18535.0
34	24648.0
35	27444.0
36	25661.0
37	29481.0
38	33403.0
39	30347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.00145372787826	24.632082346177647	26.875290654031076	15.491173271913025
2	32.09955655807038	25.656283070478338	28.921861211098133	13.322299160353143
3	29.2005229026222	25.681915551958873	31.47449018825227	13.643071357166658
4	25.85108992973038	28.694464482535125	30.106814212112447	15.347631375622045
5	22.28378086425404	33.027086209358785	29.920795632225154	14.76833729416202
6	20.893401833088603	41.41586504132322	27.38977117517604	10.300961950412134
7	87.89963784965451	3.47137034907778	7.111548897620207	1.5174429036475021
8	85.89957193755927	5.702128594497805	6.754158870120216	1.6441405978227037
9	83.15469934930115	4.859186132095163	8.966974378503869	3.0191401400998203
10	40.83510624663574	28.4963620185213	16.233050521621	14.435481213221966
11	34.27136009608518	24.4123753620588	24.314239575819048	17.002024966036963
12	31.418098728995098	21.564240491264815	28.279218282550506	18.73844249718958
13	24.044366163659735	26.636176219648394	28.93431127353153	20.385146343160336
14	20.83810890875203	28.95481725871596	29.950822253388065	20.25625157914395
15	20.25625157914395	23.09266874411826	36.5552141960006	20.095865480737192
16	23.49363399013516	20.735212804523034	34.68514158284235	21.08601162249946
17	22.762742089633125	21.691304363746884	29.614304389379363	25.931649157240628
18	24.388207593805728	22.815837944128514	31.28480982529633	21.511144636769426
19	28.122493967212392	24.439838735073657	26.482015152458338	20.95565214525561
20	29.14742704812682	24.09123698693842	26.214704988447075	20.54663097648769
21	25.614538743495757	25.65957867524012	28.649790729097628	20.076091852166495
22	25.332581447209908	23.065937727717134	27.855549981507995	23.745930843564967
23	23.89057127477654	25.94043743660538	27.341435638669893	22.827555649948188
24	23.753620588009124	23.934146493293447	31.855681805698467	20.456551112998962
25	23.70235562504806	24.441669626607982	29.534843696789714	22.321131051554243
26	21.919067270616754	27.007847201116114	27.685643247122755	23.38744228114438
27	21.813607918239708	26.320896697437853	28.683112955022317	23.182382429300123
28	21.332449623019436	26.18504454559103	31.197659388262522	21.284846443127016
29	22.544865997048603	24.398826764704808	30.89482992848538	22.161477309761217
30	23.16663676210494	25.44426583080365	30.27305916342904	21.11603824366237
31	26.772760728108945	24.96713549695889	26.96939847889531	21.29070529603685
32	27.933545960870187	24.33108377793483	26.819631551387634	20.915738709807354
33	27.259777876239056	24.908180789553665	25.818133882112555	22.01390745209472
34	23.00515212877759	26.105950031308247	28.158379441285142	22.73051839862903
35	23.513773797012718	25.69985828899524	28.27885210424364	22.507515809748398
36	26.587474504835384	27.145164066190393	24.998626831349256	21.268734597624967
37	24.093434056779607	28.68787327301156	26.277687657227812	20.94100501298102
38	24.157149082174072	28.996561585698537	25.2318824128221	21.61440691930529
39	23.667934864202774	28.108213013244672	25.219432350388697	23.00441977216386
40	24.6668692853298	25.208447001182755	27.090969676774407	23.033714036713036
41	22.01280891717413	24.936742697489116	27.33008411115709	25.72036427417967
42	23.079852503377996	26.52156240959973	25.034878483728868	25.363706603293405
43	22.746264065824214	25.464039459374348	26.98843975085228	24.801256723949162
44	22.780684826669496	25.746362933967067	28.159844154512598	23.313108084850835
45	21.68581168914391	28.434477884661156	26.179918049294926	23.699792376900007
46	23.105118806551662	27.596295740247754	26.973426440270824	22.325159012929756
47	22.716237444661303	26.441735538703213	27.29310010216375	23.548926914471732
48	23.10804823300658	25.53214862445119	28.768432500521808	22.591370642020426
49	23.159313195967645	24.99496504828061	29.090669410562782	22.755052345188968
50	22.229220296531192	26.960976377837426	27.67978439421292	23.130018931418466
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	155.0
1	247.0
2	339.0
3	1676.5
4	3014.0
5	2221.0
6	1428.0
7	1453.5
8	1479.0
9	1452.0
10	1425.0
11	1430.0
12	1435.0
13	1433.5
14	1432.0
15	1394.5
16	1357.0
17	1257.5
18	1158.0
19	1147.0
20	1136.0
21	1175.5
22	1215.0
23	1320.0
24	1425.0
25	1763.0
26	2101.0
27	2260.0
28	2419.0
29	2958.5
30	3498.0
31	4080.0
32	4662.0
33	5283.0
34	5904.0
35	6740.0
36	7576.0
37	8217.5
38	8859.0
39	9780.0
40	10701.0
41	12542.0
42	14383.0
43	15621.0
44	16859.0
45	17180.5
46	17502.0
47	17991.0
48	18480.0
49	18828.0
50	19176.0
51	18770.5
52	18365.0
53	17409.5
54	16454.0
55	15851.5
56	15249.0
57	14994.0
58	14739.0
59	14348.0
60	13957.0
61	13219.5
62	12482.0
63	11192.5
64	9903.0
65	8422.5
66	6942.0
67	6135.5
68	5329.0
69	4615.5
70	3902.0
71	3319.0
72	2736.0
73	2248.0
74	1760.0
75	1388.0
76	1016.0
77	814.5
78	613.0
79	456.0
80	299.0
81	209.5
82	120.0
83	89.0
84	58.0
85	46.0
86	34.0
87	22.5
88	11.0
89	6.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	273091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.91593695957498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.53013246584042	29.217331032820205
2	9.475847334761747	8.322814283839069
3	6.0753150864611385	8.004094630397464
4	4.505501364353832	7.914532555529677
5	3.471424701533906	7.622543417623719
6	2.6431192506027066	6.9645035031664495
7	1.9811829380138455	6.090385350784111
8	1.5138381407252801	5.318529628407327
9	1.1262379318717208	4.451381461580453
>10	2.6608019015160207	14.879079699495252
>50	0.01244916323954076	0.37468315648792655
>100	0.0033197768638775363	0.22233144499769958
>500	0.0	0.0
>1k	8.299442159693841E-4	0.6177898348706571
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1695	0.6206722301357424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011351527512807087	0.0
2	0.0	0.0	0.0	0.04540611005122835	0.0
3	0.0	0.0	0.0	0.07177094814548997	0.0
4	0.0	0.0	0.0	0.10985349205942342	0.0
5	0.0	0.0	0.0	0.17613176560194221	0.0
6	0.0	0.0	0.0	0.3573900274999908	0.0
7	0.0	0.0	0.0	0.45149785236423023	0.0
8	0.0	0.0	0.0	0.7100197370107401	0.0
9	0.0	0.0	0.0	0.837815966106536	0.0
10	0.0	0.0	0.0	1.0161448015496666	0.0
11	0.0	0.0	0.0	1.1413777824974094	0.0
12	0.0	0.0	0.0	1.2494003830225089	0.0
13	0.0	0.0	0.0	1.3156786565650278	0.0
14	0.0	0.0	0.0	1.3475361692622605	0.0
15	0.0	0.0	0.0	1.3790275036526287	0.0
16	0.0	0.0	0.0	1.425898326931316	0.0
17	0.0	0.0	0.0	1.471670615289409	0.0
18	0.0	0.0	0.0	1.5580886957094888	0.0
19	0.0	0.0	0.0	1.5914109216341805	0.0
20	0.0	0.0	0.0	1.6335214269236262	0.0
21	0.0	0.0	0.0	1.6862511031121494	0.0
22	0.0	0.0	0.0	1.7349528179251605	0.0
23	0.0	0.0	0.0	1.7983016650127612	0.0
24	0.0	0.0	0.0	1.851763697815014	0.0
25	0.0	0.0	0.0	1.8909447766495417	0.0
26	0.0	0.0	0.0	1.9323229253252578	0.0
27	0.0	0.0	0.0	1.9857849581275107	0.0
28	0.0	0.0	0.0	2.0337543163267924	0.0
29	0.0	0.0	0.0	2.0853854575947213	0.0
30	0.0	0.0	0.0	2.1593534755813995	0.0
31	0.0	0.0	0.0	2.2179420046797587	0.0
32	0.0	0.0	0.0	2.2900791311321136	0.0
33	0.0	0.0	0.0	2.351230908378526	0.0
34	0.0	0.0	0.0	2.417509181921045	0.0
35	0.0	0.0	0.0	2.4951389829763704	0.0
36	0.0	0.0	0.0	2.562881969746348	0.0
37	0.0	0.0	0.0	2.6372161660398916	0.0
38	0.0	0.0	0.0	2.7272960295286186	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	40	8.272764E-9	44.000004	20
TTAGACG	20	7.8459264E-4	44.000004	1
CGTTGAT	40	8.272764E-9	44.000004	25
TAAGTAG	20	7.8459264E-4	44.000004	1
ATTGCGT	20	7.8459264E-4	44.000004	11
CTATACG	25	4.4338994E-5	44.0	1
ACGGGTA	25	4.4338994E-5	44.0	5
CGTTTTT	1230	0.0	41.674797	1
TTACGGG	75	0.0	41.066666	3
GCCCTAC	170	0.0	40.117645	26
AAGGGTG	110	0.0	40.0	5
GTCTCAA	160	0.0	39.875004	42
ACTAATC	185	0.0	39.24324	10
TCGTTGA	45	2.339766E-8	39.11111	24
TATGGCC	45	2.339766E-8	39.11111	10
ACGGGAT	90	0.0	39.11111	5
ATCGTTG	45	2.339766E-8	39.11111	23
GTTACGG	45	2.339766E-8	39.11111	2
TAATCGT	45	2.339766E-8	39.11111	21
TACGGGA	80	0.0	38.500004	4
>>END_MODULE