FastQCFastQC Report
Thu 26 May 2016
SRR1547176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547176_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1004103
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115171.146993884093564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG408.310963E-944.01
CGTTTTT86050.042.4915731
CGGTCTA800.041.2531
TTTAGCG557.8216544E-1140.0000041
TACGTAG557.8216544E-1140.0000041
CGTTCGA501.3478711E-939.60000214
CGATTCG452.3504981E-839.1111110
CGCCGTT404.123849E-738.526
ATAGGGA9250.037.8162164
GTTTTTT97650.037.7142872
GGGCGAT23100.037.1428577
GTAGGGC5450.037.1376154
GTACGGG3150.037.0158733
GACCGAT5900.036.5423749
TAGGGCG6250.036.2565
TCGTTGA1100.036.00000424
TAATCGT1100.036.00000421
TATGCGG1650.036.02
TACGGGT800.035.754
TATAGCG800.035.751
Produced by FastQC (version 0.11.5)