##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547176_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1004103
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.52062885978829	31.0	30.0	31.0	27.0	33.0
2	30.85766400458917	31.0	31.0	33.0	28.0	34.0
3	30.979826770759573	31.0	31.0	34.0	28.0	34.0
4	34.83237675816127	35.0	35.0	37.0	32.0	37.0
5	34.65353554366435	35.0	35.0	37.0	31.0	37.0
6	34.67857779530586	35.0	35.0	37.0	31.0	37.0
7	35.32011158217832	35.0	35.0	37.0	33.0	37.0
8	34.27794558924732	35.0	35.0	37.0	32.0	37.0
9	36.36125477167183	39.0	37.0	39.0	32.0	39.0
10	35.775211308003264	37.0	35.0	39.0	30.0	39.0
11	35.58931304856175	37.0	35.0	39.0	30.0	39.0
12	35.28141933646249	37.0	34.0	39.0	30.0	39.0
13	35.19222529959576	37.0	34.0	39.0	29.0	39.0
14	36.06742634968723	38.0	34.0	40.0	29.0	41.0
15	36.186227906898	38.0	34.0	40.0	30.0	41.0
16	36.22443016304104	38.0	34.0	40.0	30.0	41.0
17	36.10606680788724	38.0	34.0	40.0	29.0	41.0
18	35.97175887334267	38.0	34.0	40.0	29.0	41.0
19	35.873484094759206	37.0	34.0	40.0	29.0	41.0
20	35.67951793790079	37.0	34.0	40.0	29.0	41.0
21	35.50470419867285	37.0	34.0	40.0	29.0	41.0
22	35.47384083107012	37.0	34.0	40.0	28.0	41.0
23	35.34199180761336	37.0	33.0	40.0	28.0	41.0
24	35.19829638991219	37.0	33.0	40.0	27.0	41.0
25	34.976051261673355	36.0	33.0	40.0	27.0	41.0
26	34.783293148212884	36.0	33.0	40.0	26.0	41.0
27	34.58008192386637	36.0	33.0	40.0	25.0	41.0
28	34.5534651325611	36.0	33.0	40.0	25.0	41.0
29	34.31505034842043	36.0	33.0	40.0	24.0	41.0
30	34.25847647103932	36.0	33.0	39.0	24.0	41.0
31	34.15667416589732	36.0	33.0	40.0	24.0	41.0
32	33.814847679969084	36.0	32.0	40.0	23.0	41.0
33	33.52662127291722	36.0	32.0	40.0	21.0	41.0
34	33.22832518177916	36.0	31.0	40.0	20.0	41.0
35	32.942748901258135	36.0	31.0	40.0	18.0	41.0
36	32.85079618326009	36.0	31.0	40.0	18.0	41.0
37	32.845011916108206	36.0	31.0	40.0	18.0	41.0
38	32.814862618675576	36.0	31.0	40.0	18.0	41.0
39	32.68525440119191	35.0	31.0	40.0	17.0	41.0
40	32.58250896571368	35.0	31.0	39.0	17.0	41.0
41	32.56192741182927	35.0	31.0	39.0	17.0	41.0
42	32.40120386056012	35.0	30.0	39.0	15.0	41.0
43	32.417087689211165	35.0	30.0	39.0	16.0	41.0
44	32.096063850023356	35.0	30.0	39.0	15.0	41.0
45	32.01000196195012	35.0	30.0	39.0	15.0	41.0
46	31.94011670117508	35.0	30.0	39.0	15.0	40.0
47	31.854623479862127	35.0	30.0	39.0	15.0	40.0
48	31.82597701630211	35.0	30.0	39.0	15.0	40.0
49	31.81722890978316	35.0	30.0	39.0	15.0	40.0
50	31.29035467476942	35.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	6.0
13	16.0
14	21.0
15	89.0
16	229.0
17	624.0
18	1264.0
19	2392.0
20	3992.0
21	5912.0
22	8548.0
23	11763.0
24	15888.0
25	21296.0
26	27381.0
27	32420.0
28	34332.0
29	37096.0
30	41187.0
31	47464.0
32	55086.0
33	65889.0
34	85026.0
35	92921.0
36	92130.0
37	103925.0
38	115730.0
39	101473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.06124969251162	22.8023419908117	24.98239722418915	19.154011092487526
2	36.24817374313193	24.16216264666075	26.51968971310712	13.069973897100198
3	28.423777241976172	24.788691996737384	33.460411929851816	13.327118831434623
4	25.054999337717344	27.262243016901657	32.957176703983556	14.725580941397446
5	21.886101326258363	30.776623513723194	32.949806942116496	14.387468217901947
6	20.078019884414246	39.46208705680593	30.608513270052974	9.851379788726854
7	83.76551011201042	3.3357135672336407	11.391261653435953	1.5075146673199862
8	81.58346305110133	5.708677297050203	10.94668574837442	1.761173903474046
9	78.92676348940299	4.638069998794944	13.258998329852615	3.176168181949461
10	37.62233555720877	27.894349484066872	20.168747628480347	14.31456733024401
11	31.20616112092086	25.099417091672866	27.406351738815637	16.288070048590633
12	28.163843749097456	21.73014123053113	31.784388653355283	18.321626367016133
13	24.501868832181557	23.726749148244753	32.932776816720995	18.838605202852694
14	21.234674132036254	26.112759348393546	33.77741128151195	18.875155238058248
15	20.73502419572494	22.663013654973643	37.319776955153	19.28218519414841
16	24.065758194129486	20.84497307547134	35.503429429052595	19.585839301346574
17	23.343322348404495	21.092358054900743	32.901604715850866	22.662714880843897
18	24.698462209554197	21.620789899044222	33.473557991560625	20.20718989984095
19	27.011372339291885	24.125413428702036	29.564994826227988	19.29821940577809
20	28.171412693717677	23.78261991050719	28.936872014126042	19.109095381649094
21	26.346101943724896	24.61779319452287	30.758497883185292	18.27760697856694
22	24.877328321895263	22.02971209128944	30.888763403754393	22.2041961830609
23	23.47199440694829	24.754233380440056	30.525254879230516	21.248517333381137
24	23.333263619369728	23.459047527992645	33.58928317114878	19.618405681488852
25	24.076215288670586	23.51750766604621	31.255658035082057	21.150619010201144
26	21.89327190537226	24.90830124001223	30.57773953468917	22.62068731992634
27	21.51711527602248	23.843171467468974	31.947320145443246	22.692393111065297
28	21.606149966686687	25.07471843028056	32.5704633887161	20.74866821431666
29	23.081695802123885	24.190944554492916	32.169707689350595	20.557651954032604
30	23.844266972611376	23.780428900222386	32.48720499789364	19.888099129272593
31	25.77773395757208	24.048528885980822	29.905298560008287	20.268438596438813
32	26.614102338106747	23.80642224951026	29.324182877652987	20.255292534730003
33	26.038762955593203	24.23874841525222	28.309346750283588	21.41314187887099
34	23.148919981316656	26.294314427902314	29.92710907147972	20.629656519301307
35	22.942965014545322	26.142437578614942	29.79027052005621	21.124326886783525
36	25.46601294887078	27.615394038261016	26.39450335274369	20.52408966012451
37	23.928222503069904	28.441205732878	27.09562664388016	20.534945120171937
38	24.403970509001567	28.343207818321424	26.341221966272382	20.911599706404623
39	23.43703783376805	27.11524614506679	26.66738372457806	22.7803322965871
40	24.571582795788878	24.791082189775352	28.443894700045714	22.19344031439006
41	22.200610893503953	25.2333674931755	28.44538857069444	24.120633042626103
42	23.309361688990073	26.775938325052312	26.367713272443165	23.54698671351445
43	22.271021996747344	25.535129364218612	28.275386090869166	23.91846254816488
44	22.482852854737015	25.545486867383126	28.807204041816426	23.164456236063433
45	22.386249219452587	26.660910285100233	28.039354528370097	22.913485967077083
46	23.213853558848047	26.57984290456258	28.08915021666104	22.117153319928335
47	22.542508089309564	26.630734097995923	28.492893657323997	22.333864155370513
48	22.347010217079326	26.070134239216497	29.682811424724353	21.900044118979825
49	23.656537227754523	25.21245330409331	29.184754950438354	21.94625451771382
50	22.2822758223011	26.024620980118573	29.23106494054893	22.4620382570314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	624.0
1	1027.5
2	1431.0
3	10950.0
4	20469.0
5	14847.0
6	9225.0
7	9388.0
8	9551.0
9	9467.5
10	9384.0
11	9458.0
12	9532.0
13	9213.0
14	8894.0
15	8366.0
16	7838.0
17	7431.0
18	7024.0
19	6638.5
20	6253.0
21	6204.0
22	6155.0
23	6164.0
24	6173.0
25	6719.0
26	7265.0
27	8211.5
28	9158.0
29	10808.5
30	12459.0
31	14242.5
32	16026.0
33	18009.5
34	19993.0
35	22944.5
36	25896.0
37	28161.5
38	30427.0
39	34176.0
40	37925.0
41	42523.5
42	47122.0
43	51574.5
44	56027.0
45	58920.0
46	61813.0
47	63399.5
48	64986.0
49	65389.5
50	65793.0
51	63426.5
52	61060.0
53	58980.0
54	56900.0
55	55998.0
56	55096.0
57	53992.0
58	52888.0
59	51968.5
60	51049.0
61	48487.0
62	45925.0
63	41404.5
64	36884.0
65	31717.5
66	26551.0
67	23231.0
68	19911.0
69	17150.5
70	14390.0
71	12405.0
72	10420.0
73	8690.0
74	6960.0
75	5547.5
76	4135.0
77	3237.0
78	2339.0
79	1765.0
80	1191.0
81	849.0
82	507.0
83	372.0
84	237.0
85	187.5
86	138.0
87	94.5
88	51.0
89	35.0
90	19.0
91	13.0
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1004103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.05813980374735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.44707706715995	28.68706204265739
2	6.85991735034613	5.3587122182394245
3	3.4648622387134496	4.059932211611848
4	2.4991708302875373	3.9045185473127186
5	2.05825979615871	4.0195899435399705
6	1.765445372394052	4.137300730250741
7	1.5199247659152268	4.155580379880643
8	1.3113553842251664	4.09752815435707
9	1.1350377509138108	3.9899216841950325
>10	5.89046857760545	34.33620219720387
>50	0.031564109332515196	0.8192796265185192
>100	0.016411779133224728	1.2411994893355738
>500	2.52488907406049E-4	0.05739536779818034
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.52488907406049E-4	1.1357774070990427
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11517	1.146993884093564	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013942792721463834	0.0
2	0.0	0.0	0.0	0.052285472705489375	0.0
3	0.0	0.0	0.0	0.10287789200908672	0.0
4	0.0	0.0	0.0	0.1563584612335587	0.0
5	0.0	0.0	0.0	0.2542567844135512	0.0
6	0.0	0.0	0.0	0.499351162181569	0.0
7	0.0	0.0	0.0	0.6521243338581799	0.0
8	0.0	0.0	0.0	1.022803437495954	0.0
9	0.0	0.0	0.0	1.2378212195362428	0.0
10	0.0	0.0	0.0	1.5055228397883484	0.0
11	0.0	0.0	0.0	1.643855261860586	0.0
12	0.0	0.0	0.0	1.7677469343284504	0.0
13	0.0	0.0	0.0	1.8487147234895225	0.0
14	0.0	0.0	0.0	1.8974149066380641	0.0
15	0.0	0.0	0.0	1.9314751574290685	0.0
16	0.0	0.0	0.0	1.9881426507041609	0.0
17	0.0	0.0	0.0	2.043515456083689	0.0
18	0.0	0.0	0.0	2.140517456874444	0.0
19	0.0	0.0	0.0	2.179557276494543	0.0
20	0.0	0.0	0.0	2.2325398888361057	0.0
21	0.0	0.0	0.0	2.2858212753074136	0.0
22	0.0	0.0	0.0	2.3434846823483246	0.0
23	0.0	0.0	0.0	2.4119039580600794	0.0
24	0.0	0.0	0.0	2.4713600098794646	0.0
25	0.0	0.0	0.0	2.5212552895469886	0.0
26	0.0	0.0	0.0	2.56995547269553	0.0
27	0.0	0.0	0.0	2.6254278694516398	0.0
28	0.0	0.0	0.0	2.680701083454586	0.0
29	0.0	0.0	0.0	2.7454354782328108	0.0
30	0.0	0.0	0.0	2.8307852879634856	0.0
31	0.0	0.0	0.0	2.9028894446087703	0.0
32	0.0021910102848014595	0.0	0.0	2.983259685510351	0.0
33	0.0021910102848014595	0.0	0.0	3.0627336040226947	0.0
34	0.0021910102848014595	0.0	0.0	3.1432034363008574	0.0
35	0.0021910102848014595	0.0	0.0	3.2375164699239023	0.0
36	0.0021910102848014595	0.0	0.0	3.320774860746358	0.0
37	0.0021910102848014595	0.0	0.0	3.409112411774489	0.0
38	0.0021910102848014595	0.0	0.0	3.5165715071063426	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	40	8.310963E-9	44.0	1
CGTTTTT	8605	0.0	42.491573	1
CGGTCTA	80	0.0	41.25	31
TTTAGCG	55	7.8216544E-11	40.000004	1
TACGTAG	55	7.8216544E-11	40.000004	1
CGTTCGA	50	1.3478711E-9	39.600002	14
CGATTCG	45	2.3504981E-8	39.11111	10
CGCCGTT	40	4.123849E-7	38.5	26
ATAGGGA	925	0.0	37.816216	4
GTTTTTT	9765	0.0	37.714287	2
GGGCGAT	2310	0.0	37.142857	7
GTAGGGC	545	0.0	37.137615	4
GTACGGG	315	0.0	37.015873	3
GACCGAT	590	0.0	36.542374	9
TAGGGCG	625	0.0	36.256	5
TCGTTGA	110	0.0	36.000004	24
TAATCGT	110	0.0	36.000004	21
TATGCGG	165	0.0	36.0	2
TACGGGT	80	0.0	35.75	4
TATAGCG	80	0.0	35.75	1
>>END_MODULE