FastQCFastQC Report
Thu 26 May 2016
SRR1547175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547175_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1100266
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126501.1497219763220894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTCG207.856334E-444.00000438
CGACGGT302.5276167E-644.00000428
GTAATCG207.856334E-444.0000041
AATCGCG302.5276167E-644.0000041
ACGGGTC454.802132E-1044.05
CGTTTTT113900.043.0535551
TCGTTGA603.6379788E-1240.33333624
CGCCGGT603.6379788E-1240.33333628
TACGGGA4100.039.170734
ACGGGCG1350.039.111115
CGTTAGG900.039.111112
GCTAACG452.3508619E-839.111111
AGGGCGA11100.038.6486476
GCGTTAG404.124322E-738.5000041
CAACGGG1850.038.0540543
ATAGGGA19200.037.9270864
ACGGGAA4300.037.8604665
GTTTTTT128200.037.6333852
AATGCGG1700.037.529412
GGGCGAT21700.037.3087587
Produced by FastQC (version 0.11.5)